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[189735_at]

Description F52E1.7 /REP_DB=WormBase Gene ID /WP=CE04635 /TR=Q20660 /GB=AAB37035.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=heat shock protein
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 21      4grp_highest_8_8 > 41      4grp_high_8_8 > 50      6grp_highest_nonorm > 22      6grp_total > 22      4grp_total > 53      6grp_highest_sub > 01_02_03      6grp_cgc > 71      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2684
1 2 3 4 range
A 3089.7
(3)
P 		2600.8
(1.7)
P 		2250.3
(0.8)
P 		778.2
(-3)
P 		2312
B 3418.4
(3)
P 		2062.7
(-0.2)
P 		2305.5
(0.4)
P 		870.8
(-3)
P 		2548
C 3461.8
(3)
P 		2130.9
(0)
P 		2329.9
(0.5)
P 		793.5
(-3)
P 		2668
avr 3323.3
(3) 		2264.8
(0.5) 		2295.2
(0.5) 		814.2
(-3) 		2509

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1229
1 2 3 4 5 6 range
A 1724.7
(0.8)
P 		2154.5
(3)
P 		2042.2
(2.4)
P 		1752.1
(1)
P 		1122.8
(-2.2)
P 		962
(-3)
P 		1193
B 1927.2
(1.7)
P 		2022
(2.1)
P 		2191.1
(3)
P 		1448.8
(-0.8)
P 		1257.1
(-1.8)
P 		1011.4
(-3)
P 		1180
C 2092.4
(2.8)
P 		2126.7
(3)
P 		2129.7
(3)
P 		1814.1
(1.2)
P 		1145.6
(-2.8)
P 		1105.4
(-3)
P 		1024
avr 1914.8
(1.9) 		2101.1
(2.9) 		2121
(3) 		1671.7
(0.5) 		1175.2
(-2.2) 		1026.3
(-3) 		1095
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1032
1 2 3 4 range
A 1125
(3)
P 		564.6
(-0.3)
P 		588.3
(-0.2)
P 		113.9
(-3)
P 		1011
B 1090.1
(3)
P 		509.4
(-0.9)
P 		534.2
(-0.7)
P 		185.6
(-3)
P 		905
C 1146
(3)
P 		612.6
(-0.2)
P 		812
(1)
P 		133.8
(-3)
P 		1012
avr 1120.4
(3) 		562.2
(-0.4) 		644.8
(0.1) 		144.4
(-3) 		976

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 541
1 2 3 4 5 6 range
A 317.9
(-2.1)
P 		766.2
(3)
P 		444.8
(-0.6)
P 		444.4
(-0.6)
P 		338.8
(-1.8)
P 		234.2
(-3)
P 		532
B 670.6
(3)
P 		673
(3)
P 		532.1
(0.1)
P 		383.7
(-2.9)
P 		510.8
(-0.3)
P 		377.1
(-3)
P 		296
C 775.6
(3)
P 		648.9
(1.1)
P 		542.1
(-0.4)
P 		364.8
(-3)
P 		370.7
(-2.9)
P 		370
(-2.9)
P 		411
avr 588
(1.2) 		696
(3) 		506.3
(-0.1) 		397.6
(-1.9) 		406.8
(-1.7) 		327.1
(-3) 		369
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189735_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11618
Sequence Type Exemplar sequence
Representative Public ID F52E1.7 NCBI
Target Description F52E1.7 /REP_DB=WormBase Gene ID /WP=CE04635 /TR=Q20660 /GB=AAB37035.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=heat shock protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028786(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028786 NCBI Caenorhabditis elegans Heat Shock Protein family member (hsp-17) (hsp-17) mRNA, complete cds. 11/11 None
SNAP00000034421 ENSEMBL cdna:SNAP chromosome:CEL140:V:8387484:8388504:-1 11/11 None
GENEFINDER00000034433 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8387484:8388504:-1 11/11 None
F52E1.7a ENSEMBL cdna:known chromosome:CEL140:V:8387029:8388517:-1 gene:F52E1.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001028787 8/11 Cross Hyb Matching Probes None
F52E1.7b 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
186113 2 1 NM_001028786 189735_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8387484-8388505(-) 100.0 100.0
Public Domain and Genome References
Ensembl F52E1.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37035 Heat shock protein protein 17, isoform a [Caenorhabditis elegans] ref|NP_001023957.1| Heat Shock Protein family member (hsp-17) [Caenorhabditis elegans] 4.0E-76
blast AAQ62453 Heat shock protein protein 17, isoform b [Caenorhabditis elegans] ref|NP_001023958.1| Heat Shock Protein family member (hsp-17) [Caenorhabditis elegans] 8.0E-75
blast CAE66059 Hypothetical protein CBG11272 [Caenorhabditis briggsae] 1.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR002068 EMBL-EBI Heat shock protein Hsp20 2.7E-33
Sequence
Target Sequence 
>C. ELEGANS:189735_AT
tccccattctttaaccatggccgcagattcttcgatgacgtcgactttgatcgccacatg
atccgaccatactgggccgatcaaacaatgctcactggacaccgagtaggagatgctatt
gatgttgtgaacaacgatcaagaatacaatgtatctgtagatgtttcacagtttgaacca
gaagagttgaaagtgaacattgttgataatcagctaatcatcgaaggaaagcataacgag
aagactgataaatatggacaggtagagcgtcactttgtccggaagtataaccttccaaca
ggggtccgtccagaacaaatcaagtccgaattgagcaacaatggagtgctcactgtcaaa
t

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCCCATTCTTTAACCATGGCCGCA 692 593 37 Antisense
TAACCATGGCCGCAGATTCTTCGAT 195 637 48 Antisense
GCCGCAGATTCTTCGATGACGTCGA 533 285 56 Antisense
CGATGACGTCGACTTTGATCGCCAC 659 249 69 Antisense
TTGATCGCCACATGATCCGACCATA 299 707 83 Antisense
TGATCCGACCATACTGGGCCGATCA 80 565 95 Antisense
GGCCGATCAAACAATGCTCACTGGA 116 543 111 Antisense
TGCTCACTGGACACCGAGTAGGAGA 267 579 125 Antisense
AGAGCGTCACTTTGTCCGGAAGTAT 420 69 300 Antisense
CAACAGGGGTCCGTCCAGAACAAAT 46 189 332 Antisense
AACAATGGAGTGCTCACTGTCAAAT 330 135 373 Antisense
Sequence Source Affymetrix Proprietary Database