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[189728_s_at]

Description F53B6.2 /REP_DB=WormBase Gene ID /WP=CE20845 /TR=P90884 /GB=CAB03121.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=thrombospondin like
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 33      4grp_highest_8_8 > 0      4grp_high_8_8 > 44      6grp_highest_nonorm > 22      6grp_total > 21      4grp_total > 51      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1490
1 2 3 4 range
A 1650.8
(-0.4)
P 		2363.1
(3)
P 		1493.5
(-1.2)
P 		1123.8
(-3)
P 		1239
B 1757.2
(1.4)
P 		2090.2
(3)
P 		1679.4
(1)
P 		873.1
(-3)
P 		1217
C 1639.9
(0.6)
P 		2162.6
(3)
P 		1759
(1.1)
P 		873.9
(-3)
P 		1289
avr 1682.6
(0.5) 		2205.3
(3) 		1644
(0.3) 		956.9
(-3) 		1248

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1824
1 2 3 4 5 6 range
A 2060.8
(1.3)
P 		2589.7
(3)
P 		1805.8
(0.4)
P 		1513.5
(-0.5)
P 		1204.6
(-1.6)
P 		765.3
(-3)
P 		1824
B 1712.6
(-0.1)
P 		2549.2
(3)
P 		2034.9
(1.1)
P 		1630.1
(-0.4)
P 		1203.5
(-1.9)
P 		912.7
(-3)
P 		1637
C 1477.9
(-0.8)
P 		2486.4
(3)
P 		2107.9
(1.6)
P 		1633.5
(-0.2)
P 		1052.4
(-2.4)
P 		894.8
(-3)
P 		1592
avr 1750.4
(0.2) 		2541.8
(3) 		1982.9
(1) 		1592.4
(-0.4) 		1153.5
(-1.9) 		857.6
(-3) 		1684
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 474
1 2 3 4 range
A 601.1
(3)
P 		513
(1.8)
P 		390.4
(0.1)
P 		164.5
(-3)
P 		437
B 560.3
(3)
P 		516.2
(2.3)
P 		389.1
(0.3)
P 		186.1
(-3)
P 		374
C 542.9
(2)
P 		621.8
(3)
P 		613.1
(2.9)
P 		147.4
(-3)
P 		474
avr 568.1
(3) 		550.3
(2.7) 		464.2
(1.4) 		166
(-3) 		402

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 735
1 2 3 4 5 6 range
A 379.9
(-1.4)
P 		921
(3)
P 		393.3
(-1.3)
P 		383.9
(-1.4)
P 		363.5
(-1.6)
P 		186.3
(-3)
P 		735
B 595.9
(0)
P 		848.5
(3)
P 		494.1
(-1.2)
P 		431.8
(-1.9)
P 		489
(-1.2)
P 		340.3
(-3)
P 		508
C 547.8
(0.2)
P 		758.7
(3)
P 		536.5
(0.1)
P 		328.5
(-2.6)
P 		340.5
(-2.5)
P 		299.6
(-3)
P 		459
avr 507.9
(-0.5) 		842.7
(3) 		474.6
(-0.9) 		381.4
(-1.9) 		397.7
(-1.7) 		275.4
(-3) 		567
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189728_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1252
Sequence Type Exemplar sequence
Representative Public ID F53B6.2 NCBI
Target Description F53B6.2 /REP_DB=WormBase Gene ID /WP=CE20845 /TR=P90884 /GB=CAB03121.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=thrombospondin like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182084(11), NM_060004(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182084 NCBI Caenorhabditis elegans F53B6.2b (F53B6.2) mRNA, complete cds. 11/11 A 
NM_060004 NCBI Caenorhabditis elegans F53B6.2a (F53B6.2) mRNA, complete cds. 11/11 A 
SNAP00000012064 ENSEMBL cdna:SNAP chromosome:CEL140:I:8939506:8943278:-1 11/11 None
GENEFINDER00000012077 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8939506:8948397:-1 11/11 None
F53B6.2b ENSEMBL cdna:known chromosome:CEL140:I:8939164:8945775:-1 gene:F53B6.2 11/11 A 
F53B6.2a ENSEMBL cdna:known chromosome:CEL140:I:8939287:8950382:-1 gene:F53B6.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8939217-8950259(-) 100.0 100.0
Public Domain and Genome References
Ensembl F53B6.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD56586 Hypothetical protein F53B6.2b [Caenorhabditis elegans] ref|NP_871884.1| F53B6.2b [Caenorhabditis elegans] 0.0
blast CAB03121 Hypothetical protein F53B6.2a [Caenorhabditis elegans] ref|NP_492405.3| F53B6.2a [Caenorhabditis elegans] 0.0
blast CAE66996 Hypothetical protein CBG12394 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.0E-5
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 7.6E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.0E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.0E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.0E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492405.3 1 7-29
F53B6.2a 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:189728_S_AT
gtgaatcacttgcatcagttcgtccaccagaaactcgtccatgtcatcgagaagattgtc
caagatgggaagcatctcaatggtcagaatgttcgtctcaacgttgtgtaagcagtatgt
tggcacaaaaacgacgaaatgttacatgccgattcacaaacggaacatctgtggacattc
aacattgtgatattacaaatagaccagctaccactatggattgtccaaatcaaaactgta
aagcggaatggagaacctcagactggggttcatgtagttcagaatgtggtacaggaggtg
ttcaacttcgtcttctctcctgtgtatggatatcttctggacgtccagcaggcagaaatt
gtgaacaaatgcgtcgaccgcattctgcaagagcatgtgttgccgatgaaccacttccac
cgtgtatgccaactgcaagtgctctttaccaaagagacgcctcatgccaagatcagtccc
gattctgtgacatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGAATCACTTGCATCAGTTCGTCC 357 481 2612 Antisense
CACCAGAAACTCGTCCATGTCATCG 171 203 2636 Antisense
GACCAGCTACCACTATGGATTGTCC 97 385 2813 Antisense
AGGAGGTGTTCAACTTCGTCTTCTC 160 57 2904 Antisense
TCGTCTTCTCTCCTGTGTATGGATA 338 605 2919 Antisense
GTCGACCGCATTCTGCAAGAGCATG 163 475 2984 Antisense
GCATGTGTTGCCGATGAACCACTTC 66 311 3004 Antisense
TTCCACCGTGTATGCCAACTGCAAG 310 699 3026 Antisense
GCCAACTGCAAGTGCTCTTTACCAA 149 279 3039 Antisense
AAAGAGACGCCTCATGCCAAGATCA 200 123 3062 Antisense
AGATCAGTCCCGATTCTGTGACATT 548 65 3081 Antisense
Sequence Source Affymetrix Proprietary Database