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[189725_at]

Description K08E5.2 /REP_DB=WormBase Gene ID /WP=CE01602 /TR=SW:Q21339 /GB=CAA83225.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Yeast ORF YCR37C
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 16      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 60      6grp_total > 25      4grp_total > 38      6grp_highest_sub > De      6grp_cgc > 23      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 685
1 2 3 4 range
A 990.3
(3)
P 		376.2
(-3)
P 		410.7
(-2.7)
P 		470.5
(-2.1)
P 		614
B 902.1
(3)
P 		527
(-0.8)
P 		305.2
(-3)
P 		521.8
(-0.8)
P 		597
C 780.7
(3)
P 		656.3
(1)
P 		398.1
(-3)
P 		482.6
(-1.7)
P 		383
avr 891
(3) 		519.8
(-1.3) 		371.3
(-3) 		491.6
(-1.6) 		520

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 366
1 2 3 4 5 6 range
A 470.7
(3)
P 		395.3
(1.5)
P 		254.9
(-1.4)
P 		234
(-1.8)
P 		257.2
(-1.3)
P 		174.2
(-3)
P 		297
B 539.7
(3)
P 		419.4
(0.4)
P 		338.3
(-1.3)
P 		274.2
(-2.7)
P 		283.8
(-2.5)
P 		259.4
(-3)
P 		280
C 459.9
(2.7)
P 		475.6
(3)
P 		300.3
(-0.5)
P 		287.7
(-0.7)
P 		173.6
(-3)
P 		343.4
(0.4)
P 		302
avr 490.1
(3) 		430.1
(1.6) 		297.8
(-1.6) 		265.3
(-2.4) 		238.2
(-3) 		259
(-2.5) 		252
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 292
1 2 3 4 range
A 360.6
(3)
P 		81.7
(-2.7)
P 		107.4
(-2.2)
P 		68.9
(-3)
P 		292
B 287.7
(3)
P 		130.2
(-1.4)
P 		70.7
(-3)
P 		111.2
(-1.9)
P 		217
C 258.4
(3)
P 		188.7
(0.6)
P 		138.7
(-1.1)
P 		81.4
(-3)
P 		177
avr 302.2
(3) 		133.5
(-1.7) 		105.6
(-2.5) 		87.2
(-3) 		215

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 145
1 2 3 4 5 6 range
A 86.8
(-0.3)
P 		140.6
(3)
P 		55.5
(-2.2)
P 		59.3
(-2)
P 		77.6
(-0.8)
P 		42.4
(-3)
P 		98
B 187.8
(3)
P 		139.6
(0.5)
P 		82.1
(-2.5)
P 		72.6
(-3)
P 		115.3
(-0.8)
P 		96.7
(-1.7)
P 		115
C 170.4
(3)
P 		145.1
(1.7)
P 		76.4
(-1.9)
P 		57.8
(-2.9)
P 		56.2
(-3)
P 		115
(0.1)
P 		114
avr 148.3
(3) 		141.8
(2.5) 		71.3
(-2.4) 		63.2
(-3) 		83
(-1.6) 		84.7
(-1.5) 		85
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189725_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7324
Sequence Type Exemplar sequence
Representative Public ID K08E5.2 NCBI
Target Description K08E5.2 /REP_DB=WormBase Gene ID /WP=CE01602 /TR=SW:Q21339 /GB=CAA83225.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Yeast ORF YCR37C
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027502(11), NM_181984(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027502 NCBI Caenorhabditis elegans NADC (Na+-coupled dicarboxylate transporter) family member (nac-3) (nac-3) mRNA, complete cds. 11/11 None
NM_181984 NCBI Caenorhabditis elegans NADC (Na+-coupled dicarboxylate transporter) family member (nac-3) (nac-3) mRNA, complete cds. 11/11 None
GENEFINDER00000037016 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10152737:10157338:1 11/11 None
SNAP00000037010 ENSEMBL cdna:SNAP chromosome:CEL140:III:10155298:10157338:1 11/11 None
K08E5.2a ENSEMBL cdna:known chromosome:CEL140:III:10152682:10157812:1 gene:K08E5.2 11/11 A 
K08E5.2b.1 ENSEMBL cdna:known chromosome:CEL140:III:10152684:10157338:1 gene:K08E5.2 11/11 None
K08E5.2b.2 ENSEMBL cdna:known chromosome:CEL140:III:10154009:10157338:1 gene:K08E5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10153983-10157334(+) 100.0 100.0
Public Domain and Genome References
Ensembl K08E5.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA83225 Hypothetical protein K08E5.2a [Caenorhabditis elegans] ref|NP_001022673.1| NADC (Na+-coupled dicarboxylate transporter) family member (nac-3) [Caenorhabditis elegans] gb|AAM11894.1| high-affinity plasma membrane transporter INDY2 [Caenorhabditis elegans] sp|Q21339|NAD3_CAEEL Sodium-dependent high-affinity dicarboxylate transporter 3 (Na(+)/dicarboxylate cotransporter 3) (NaDC-3) (ceNaDC3) 0.0
blast CAE64963 Hypothetical protein CBG09797 [Caenorhabditis briggsae] 0.0
blast G88575 protein K08E5.2 [imported] - Caenorhabditis elegans 0.0
blast CAD82916 Hypothetical protein K08E5.2b [Caenorhabditis elegans] ref|NP_871713.1| NADC (Na+-coupled dicarboxylate transporter) family member (nac-3) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001898 EMBL-EBI Sodium/sulphate symporter 2.9E-9
Pfam IPR001898 EMBL-EBI Sodium/sulphate symporter 2.3E-18
Pfam IPR001898 EMBL-EBI Sodium/sulphate symporter 5.1E-13
Pfam IPR001898 EMBL-EBI Sodium/sulphate symporter 6.2E-18
Pfam IPR001898 EMBL-EBI Sodium/sulphate symporter 5.1E-13
Pfam IPR001898 EMBL-EBI Sodium/sulphate symporter 1.5E-39
Pfam IPR001898 EMBL-EBI Sodium/sulphate symporter 5.1E-13
Pfam IPR001898 EMBL-EBI Sodium/sulphate symporter 6.1E-38
Pfam IPR001898 EMBL-EBI Sodium/sulphate symporter 5.1E-13
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022673.1 12 51-70,90-112,125-139,162-179,220-242,266-288,318-337,352-371,401-420,450-472,484-506,521-543
NP_871713.1 11 5-24,37-51,74-91,132-154,178-200,230-249,264-283,313-332,362-384,396-418,433-455
K08E5.2a 12 51-70,90-112,125-139,162-179,220-242,266-288,318-337,352-371,401-420,450-472,484-506,521-543
K08E5.2b.1 11 5-24,37-51,74-91,132-154,178-200,230-249,264-283,313-332,362-384,396-418,433-455
K08E5.2b.2 11 5-24,37-51,74-91,132-154,178-200,230-249,264-283,313-332,362-384,396-418,433-455
GENEFINDER00000037016 10 51-70,90-112,230-252,276-298,328-347,362-381,411-430,460-482,494-516,531-553
SNAP00000037010 8 5-24,48-70,100-119,134-153,183-202,232-254,266-288,303-325
Sequence
Target Sequence 
>C. ELEGANS:189725_AT
tcctgtttgttcttccatctgaaagacccgacttggcaacttatatcaagaaagaagatt
tgaaaaagagaggatgtctgatggattggaaaacaatgcaggaaacattcccatggagtg
ttgtacttcttttgggtggaggttttgcattggctgctggagttaaagaatctggattgt
cacttttaattggaaatagtctatcttctattgaacatcttccactttggattcttcaat
tattaactatgctcattgcaatggtcatcacaaatatctgctcaaacactgtcactgctt
caatttttgttccgattgttgcaacacttgctcaaagagctggacatcacccattcaccc
tcatgcttccaaccacattggcttcatctttcgcattcattttcccagttggaacaccac
caaatgcaattgtgtttggaagtggaatggtgaaagtttctgatatggcattcgttggcg
gtatcatttcattggaacttttggtattgacagttctctacatgaatagtattgcatact
tgactcttccattac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCTGTTTGTTCTTCCATCTGAAAG 408 591 1013 Antisense
GAAAGACCCGACTTGGCAACTTATA 565 355 1033 Antisense
GAGTGTTGTACTTCTTTTGGGTGGA 279 401 1128 Antisense
GGGTGGAGGTTTTGCATTGGCTGCT 676 497 1146 Antisense
ATATCTGCTCAAACACTGTCACTGC 440 19 1286 Antisense
GTCACTGCTTCAATTTTTGTTCCGA 576 465 1303 Antisense
GTTCCGATTGTTGCAACACTTGCTC 612 441 1321 Antisense
CAAAGAGCTGGACATCACCCATTCA 220 191 1345 Antisense
CAACCACATTGGCTTCATCTTTCGC 193 187 1382 Antisense
GGCATTCGTTGGCGGTATCATTTCA 390 537 1479 Antisense
ATTGCATACTTGACTCTTCCATTAC 339 5 1543 Antisense
Sequence Source Affymetrix Proprietary Database