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[189724_at]

Description K04G7.2 /REP_DB=WormBase Gene ID /WP=CE25041 /TR=Q21231 /GB=AAA62534.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Glutamate receptor
Cluster 6grp_total > 14      4grp_total > 61      6grp_cgc > 23      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 23.9
(-1.3)
A 		45.1
(1)
A 		63
(3)
A 		8
(-3)
A 		55
B 77
(3)
A 		54.2
(-0.2)
A 		34.7
(-3)
A 		53.6
(-0.3)
A 		42
C 72.8
(3)
A 		44.3
(0.2)
A 		42.6
(0)
A 		11.8
(-3)
A 		61
avr 57.9
(3) 		47.9
(1.2) 		46.8
(1) 		24.5
(-3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 67.5
(3)
A 		35
(0)
A 		2.1
(-3)
A 		6.2
(-2.6)
A 		10.3
(-2.2)
A 		3.1
(-2.9)
A 		65
B 35
(2.5)
A 		15.9
(-1.8)
A 		29.1
(1.2)
A 		37.3
(3)
A 		23.4
(-0.1)
A 		10.5
(-3)
A 		27
C 72.2
(0.9)
A 		102.6
(3)
A 		49.8
(-0.7)
A 		60
(0)
A 		16.9
(-3)
A 		37.4
(-1.6)
A 		86
avr 58.2
(3) 		51.2
(2) 		27
(-1.5) 		34.5
(-0.4) 		16.9
(-3) 		17
(-3) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 8.7
(-0.1)
A 		9.8
(0.4)
A 		16.5
(3)
A 		1.2
(-3)
A 		15
B 24.6
(3)
A 		13.4
(-1)
A 		8
(-3)
A 		11.4
(-1.8)
A 		17
C 24.1
(3)
A 		12.7
(-0.1)
A 		14.8
(0.5)
A 		2
(-3)
A 		22
avr 19.1
(3) 		12
(0) 		13.1
(0.5) 		4.9
(-3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 12.4
(3)
A 		12.4
(3)
A 		0.5
(-3)
A 		1.6
(-2.4)
A 		3.1
(-1.7)
A 		0.8
(-2.8)
A 		12
B 12.2
(3)
A 		5.3
(-2)
A 		7.1
(-0.7)
A 		9.9
(1.3)
A 		9.5
(1)
A 		3.9
(-3)
A 		8
C 26.8
(2)
A 		31.3
(3)
A 		12.7
(-1.3)
A 		12.1
(-1.5)
A 		5.5
(-3)
A 		12.5
(-1.4)
A 		26
avr 17.1
(3) 		16.3
(2.6) 		6.8
(-2.4) 		7.9
(-1.8) 		6
(-2.8) 		5.7
(-3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189724_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7663
Sequence Type Exemplar sequence
Representative Public ID K04G7.2 NCBI
Target Description K04G7.2 /REP_DB=WormBase Gene ID /WP=CE25041 /TR=Q21231 /GB=AAA62534.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Glutamate receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001025942(11), NM_001025943(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001025942 NCBI Caenorhabditis elegans GLutamate Receptor family (AMPA) family member (glr-2) (glr-2) mRNA, complete cds. 11/11 None
NM_001025943 NCBI Caenorhabditis elegans GLutamate Receptor family (AMPA) family member (glr-2) (glr-2) mRNA, complete cds. 11/11 None
SNAP00000036052 ENSEMBL cdna:SNAP chromosome:CEL140:III:7142862:7144089:-1 10/11 None
GENEFINDER00000036059 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7143152:7144343:-1 11/11 None
B0280.12b ENSEMBL cdna:known chromosome:CEL140:III:7138607:7144756:-1 gene:B0280.12 11/11 A 
B0280.12a ENSEMBL cdna:known chromosome:CEL140:III:7138607:7144756:-1 gene:B0280.12 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7143149-7144754(-) 100.0 100.0
Public Domain and Genome References
Ensembl B0280.12
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA19090 Glutamate receptor family (ampa) protein 2, isoform a [Caenorhabditis elegans] ref|NP_001021113.1| GLutamate Receptor family (AMPA) family member (glr-2) [Caenorhabditis elegans] 0.0
blast AAO61447 Glutamate receptor family (ampa) protein 2, isoform b [Caenorhabditis elegans] ref|NP_001021114.1| GLutamate Receptor family (AMPA) family member (glr-2) [Caenorhabditis elegans] gb|AAK01094.2| non-NMDA ionotropic glutamate receptor subunit GLR-2 [Caenorhabditis elegans] sp|Q10914|GLR2_CAEEL Glutamate receptor 2 precursor 0.0
blast CAE70142 Hypothetical protein CBG16604 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001320 EMBL-EBI Ionotropic glutamate receptor 1.0E-126
Pfam IPR001320 EMBL-EBI Ionotropic glutamate receptor 1.0E-126
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 1.0E-6
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021113.1 3 553-575,627-649,832-854
NP_001021114.1 3 622-644,696-718,901-923
B0280.12b 3 622-644,696-718,901-923
B0280.12a 3 553-575,627-649,832-854
Sequence
Target Sequence 
>C. ELEGANS:189724_AT
atgatacagcccatgccattattgaaatgctcagattggcggaaatgacattcaacgcgt
tgtccgatgtcgattttgacgtacttttgggtacgagagatcttccgccaatggaaatgg
caacaatgatgtggaatttgaatcgaataatctgtgatgaaatgaaactgggatatatgc
tcatgttggctggtactaatttcaaaaattatggtatttatgaagatattgctaatcata
tgaagatgcctcttatcgactgggagccttcaaaatctgaaaatataggtaaaacgactg
aaaataatccgatgatattttcggttgctccatcagctgagcagcttttaattgattata
ttcaatacaaaggatggagagatgttgtatatattcatgacggaaaaaatgctgatcgca
ctttacgtacaatgttctcatatctgcacgagaaatctccgaaatatcagttattcgttg
ataattatgtggcaccatcagacgaagagatgttcaaagaatttttaaacgaatttcatc
ggagaatatcgacacaacacacat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGATACAGCCCATGCCATTATTGA 95 47 119 Antisense
TTCAACGCGTTGTCCGATGTCGATT 602 685 169 Antisense
TGGGTACGAGAGATCTTCCGCCAAT 478 553 206 Antisense
ATATGCTCATGTTGGCTGGTACTAA 521 21 293 Antisense
GAAGATGCCTCTTATCGACTGGGAG 170 339 360 Antisense
TCGACTGGGAGCCTTCAAAATCTGA 44 601 374 Antisense
GGTTGCTCCATCAGCTGAGCAGCTT 38 509 441 Antisense
ATGCTGATCGCACTTTACGTACAAT 347 41 527 Antisense
CTCATATCTGCACGAGAAATCTCCG 171 229 555 Antisense
TATGTGGCACCATCAGACGAAGAGA 52 655 604 Antisense
CGGAGAATATCGACACAACACACAT 585 243 658 Antisense
Sequence Source Affymetrix Proprietary Database