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[189709_at] Carbonic anhydrase

Description D1022.8 /REP_DB=WormBase Gene ID /WP=CE02582 /TR=Q18932 /GB=AAB93341.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Carbonic anhydrase
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 12      4grp_highest_8_8 > 59      4grp_high_8_8 > 26      6grp_highest_nonorm > 60      6grp_total > 23      4grp_total > 38      6grp_highest_sub > De      6grp_cgc > 30      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 443
1 2 3 4 range
A 648.2
(3)
P 		364.9
(-1.1)
P 		230.3
(-3)
P 		387.2
(-0.7)
P 		418
B 609
(3)
P 		442.3
(0.3)
P 		234.1
(-3)
P 		364.1
(-0.9)
P 		375
C 673.3
(3)
P 		365.2
(-2.2)
P 		316.3
(-3)
P 		386.9
(-1.8)
P 		357
avr 643.5
(3) 		390.8
(-1) 		260.2
(-3) 		379.4
(-1.1) 		383

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 439
1 2 3 4 5 6 range
A 581
(3)
P 		524.3
(2.2)
P 		470.1
(1.5)
P 		355.1
(-0.1)
P 		177.6
(-2.6)
P 		146.4
(-3)
P 		435
B 552.6
(3)
P 		545.1
(2.9)
P 		500.3
(2.1)
P 		315.9
(-1.2)
P 		284.3
(-1.7)
P 		210.5
(-3)
P 		342
C 584.9
(3)
P 		502.3
(1.7)
P 		405.7
(0.2)
P 		389.3
(-0.1)
P 		201.3
(-3)
P 		220.7
(-2.7)
P 		384
avr 572.8
(3) 		523.9
(2.2) 		458.7
(1.2) 		353.4
(-0.5) 		221.1
(-2.5) 		192.5
(-3) 		380
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 182
1 2 3 4 range
A 236
(3)
P 		79.2
(-2.2)
P 		60.2
(-2.9)
P 		56.7
(-3)
P 		179
B 194.2
(3)
P 		109.2
(-0.6)
P 		54.3
(-3)
P 		77.6
(-2)
P 		140
C 222.9
(3)
P 		105
(-1.5)
P 		110.2
(-1.3)
P 		65.2
(-3)
P 		158
avr 217.7
(3) 		97.8
(-1.8) 		74.9
(-2.7) 		66.5
(-3) 		151

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 181
1 2 3 4 5 6 range
A 107.1
(-0.2)
P 		186.5
(3)
P 		102.4
(-0.3)
P 		90.1
(-0.8)
P 		53.6
(-2.3)
P 		35.6
(-3)
P 		151
B 192.3
(3)
P 		181.4
(2.4)
P 		121.5
(-0.7)
P 		83.7
(-2.7)
P 		115.5
(-1)
P 		78.5
(-3)
P 		114
C 216.8
(3)
P 		153.3
(0.5)
P 		103.3
(-1.5)
P 		78.3
(-2.5)
P 		65.1
(-3)
P 		73.9
(-2.7)
P 		152
avr 172.1
(2.9) 		173.7
(3) 		109.1
(-0.5) 		84
(-1.8) 		78.1
(-2.2) 		62.7
(-3) 		111
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189709_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4219
Sequence Type Exemplar sequence
Representative Public ID D1022.8 NCBI
Target Description D1022.8 /REP_DB=WormBase Gene ID /WP=CE02582 /TR=Q18932 /GB=AAB93341.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Carbonic anhydrase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063166(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063166 NCBI Caenorhabditis elegans Carbonic AnHydrase family member (cah-2) (cah-2) mRNA, complete cds. 10/11 A 
SNAP00000004650 ENSEMBL cdna:SNAP chromosome:CEL140:II:7480843:7483017:-1 10/11 None
GENEFINDER00000004655 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7480843:7485324:-1 11/11 None
D1022.8 ENSEMBL cdna:known chromosome:CEL140:II:7480739:7483035:-1 gene:D1022.8 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7480839-7482426(-) 97.85 100.0
Public Domain and Genome References
Gene Title Carbonic anhydrase
Gene Symbol cah-2
Ensembl D1022.8
Entrez Gene 174218 Entrez gene
SwissProt Q18932 EMBL-EBI
WormBase CE31777 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1628885_AT dm
DROSGENOME1:153025_AT dm
DROSGENOME1:144767_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6730 one-carbon compound metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4089 carbonate dehydratase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_495567 Carbonic AnHydrase family member (cah-2) [Caenorhabditis elegans] gb|AAB93341.3| Carbonic anhydrase protein 2 [Caenorhabditis elegans] sp|Q18932|CAH2_CAEEL Putative carbonic anhydrase-like protein 2 precursor 0.0
blast CAE67520 Hypothetical protein CBG13040 [Caenorhabditis briggsae] 0.0
blast T34196 hypothetical protein D1022.8 - Caenorhabditis elegans 0.0
blast T34196 hypothetical protein D1022.8 - Caenorhabditis elegans 1.0E-175
Protein Domains
Database ID Description E-Value
Pfam IPR001148 EMBL-EBI Carbonic anhydrase, eukaryotic 6.4E-38
Pfam IPR001148 EMBL-EBI Carbonic anhydrase, eukaryotic 6.4E-38
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000004655 1 5-27
Sequence
Target Sequence 
>C. ELEGANS:189709_AT
aagagggcttcttgcagtttctgtaattgttgatattggaaagactacatctgtggagtt
acgaagattaacggttgcgtcacagagcattaactataaaggccagactacaaatctcac
tgattttcaaccatctgcacttttaccaaaaacctctcactatgtgacatacgaaggatc
tttaacttttcccggatgtcatgaaactgtcacttgggttattttaaataatccaattta
tataaccaatgatgatcttcaaatttggaatgaaatgcaaaaaacagaaacaaaacaacc
agaaccatcatatatgactcctgcatatcgtccattgaaatcgttaaatggccgtctcgt
tagaacaaatataaatgttggatcaaaacagtctactgtatcttcaagttgtccatcgaa
tgtttacgtggaaatggggtaccaagcgaatcctggtagaaataagcggaatgattcggt
aagatacacgtcgcaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGAGGGCTTCTTGCAGTTTCTGTA 421 153 378 Antisense
AACGGTTGCGTCACAGAGCATTAAC 606 143 447 Antisense
TAAAGGCCAGACTACAAATCTCACT 328 639 474 Antisense
ATCTCACTGATTTTCAACCATCTGC 16 33 491 Antisense
TTCAACCATCTGCACTTTTACCAAA 83 685 503 Antisense
TACCAAAAACCTCTCACTATGTGAC 72 637 521 Antisense
GATCTTTAACTTTTCCCGGATGTCA 154 419 554 Antisense
GAACCATCATATATGACTCCTGCAT 682 345 679 Antisense
ACTCCTGCATATCGTCCATTGAAAT 521 99 694 Antisense
AATCGTTAAATGGCCGTCTCGTTAG 414 165 716 Antisense
GATTCGGTAAGATACACGTCGCAGA 627 425 850 Antisense
Sequence Source Affymetrix Proprietary Database