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[189690_s_at]

Description T14G8.3 /REP_DB=WormBase Gene ID /WP=CE03659 /TR=Q22515 /GB=CAA91809.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=heat shock protein
Cluster 6grp_highest_10_10 > 12      6grp_high1_10_10 > 02      4grp_highest_8_8 > 18      4grp_high_8_8 > 63      6grp_highest_nonorm > 51      6grp_total > 45      4grp_total > 56      6grp_high1_sub > De      6grp_total_sub > Cup      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 929
1 2 3 4 range
A 556.5
(0.6)
P 		525
(0)
P 		692.2
(3)
P 		356
(-3)
P 		336
B 506.5
(-1.2)
P 		589.2
(-0.1)
P 		809.6
(3)
P 		376.2
(-3)
P 		433
C 629.6
(-1.1)
P 		584.5
(-1.4)
P 		1267.7
(3)
P 		338.6
(-3)
P 		929
avr 564.2
(-0.8) 		566.2
(-0.8) 		923.2
(3) 		356.9
(-3) 		566

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 655
1 2 3 4 5 6 range
A 574.4
(-1.5)
P 		668.4
(-0.3)
P 		935
(3)
P 		484.6
(-2.6)
P 		455.8
(-3)
P 		570.2
(-1.6)
P 		479
B 979.2
(3)
P 		588.4
(-0.7)
P 		677.4
(0.2)
P 		712.9
(0.5)
P 		340.5
(-3)
P 		683.5
(0.2)
P 		639
C 750
(3)
P 		575.2
(0.5)
P 		544.5
(0.1)
P 		544.5
(0.1)
P 		324.1
(-3)
P 		557.1
(0.3)
P 		426
avr 767.9
(3) 		610.7
(0.6) 		719
(2.3) 		580.7
(0.2) 		373.5
(-3) 		603.6
(0.5) 		394
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 390
1 2 3 4 range
A 202.6
(3)
P 		114
(-0.5)
P 		181
(2.1)
P 		52.1
(-3)
P 		151
B 161.5
(1.5)
P 		145.5
(0.6)
P 		187.6
(3)
P 		80.2
(-3)
P 		107
C 208.4
(-0.6)
P 		168.1
(-1.3)
P 		441.8
(3)
P 		57.1
(-3)
P 		385
avr 190.8
(0.7) 		142.5
(-0.7) 		270.1
(3) 		63.1
(-3) 		207

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 236
1 2 3 4 5 6 range
A 105.9
(-3)
P 		237.7
(3)
P 		203.6
(1.4)
P 		122.9
(-2.2)
P 		137.5
(-1.6)
P 		138.8
(-1.5)
P 		132
B 340.7
(3)
P 		195.9
(-1.3)
P 		164.5
(-2.2)
P 		188.8
(-1.5)
P 		138.3
(-3)
P 		254.9
(0.5)
P 		202
C 278
(3)
P 		175.5
(-0.6)
P 		138.6
(-1.8)
P 		109.5
(-2.8)
P 		104.9
(-3)
P 		186.5
(-0.2)
P 		173
avr 241.5
(3) 		203
(1) 		168.9
(-0.8) 		140.4
(-2.3) 		126.9
(-3) 		193.4
(0.5) 		115
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189690_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17817
Sequence Type Exemplar sequence
Representative Public ID T14G8.3 NCBI
Target Description T14G8.3 /REP_DB=WormBase Gene ID /WP=CE03659 /TR=Q22515 /GB=CAA91809.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=heat shock protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029742(11), NM_001029743(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029742 NCBI Caenorhabditis elegans T14G8.3a (T14G8.3) mRNA, complete cds. 11/11 None
NM_001029743 NCBI Caenorhabditis elegans T14G8.3b (T14G8.3) mRNA, complete cds. 11/11 None
SNAP00000007537 ENSEMBL cdna:SNAP chromosome:CEL140:X:12865365:12869392:1 11/11 None
GENEFINDER00000007541 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:12865383:12869392:1 11/11 None
T14G8.3a ENSEMBL cdna:known chromosome:CEL140:X:12861156:12869914:1 gene:T14G8.3 11/11 None
T14G8.3b ENSEMBL cdna:known chromosome:CEL140:X:12861156:12869392:1 gene:T14G8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:12861156-12869392(+) 94.28 100.0
Public Domain and Genome References
Ensembl T14G8.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91809 Hypothetical protein T14G8.3a [Caenorhabditis elegans] ref|NP_001024913.1| T14G8.3a [Caenorhabditis elegans] 0.0
blast CAH60753 Hypothetical protein T14G8.3b [Caenorhabditis elegans] ref|NP_001024914.1| T14G8.3b [Caenorhabditis elegans] 0.0
blast CAE57201 Hypothetical protein CBG00051 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 2.5E-19
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 3.1E-27
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 2.5E-19
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 3.1E-27
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 9.5E-13
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 3.1E-27
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 9.5E-13
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 4.2E-25
Sequence
Target Sequence 
>C. ELEGANS:189690_S_AT
tcgaggatgacgtttcgctcgaaacgaagaaagaagaatttgatgagaagagagcaaagt
tggaagagttgacagctaaaccaaatgccagaaagcaagaaagacttgatattccaaaag
ttgctgaaattatcgaggatcacttcaatcgttctatgaccttccacgctatggctctta
acttgacacagtttgaggagggtaacaagacattcacagacactgaacttgaggttctca
ctaaattgattgagtcgacgaccgagtggtggaatgagaagaaggatcttttcgagaagc
aagctaagaacgaggatcccgttgtgaaagcttctgagattgcagaaaaggctcgtgact
tggaacgagaagtgcgttatctggtgaataagttgaagattgcaagctccaagaagagca
aagaatctgaaaagaaaaagaagacaaagaaggaaaaagacactgtaaaagaaagaaagg
aggaagaaaccacagaaacaactacagaggatcaagctaatgagcagcagactcctccgc
cgtttgagaacgataacacaaaggatgatgaatcagcatcgactgaagagccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGAGGATGACGTTTCGCTCGAAAC 201 603 2165 Antisense
GGATCACTTCAATCGTTCTATGACC 182 511 2301 Antisense
TTCAATCGTTCTATGACCTTCCACG 478 685 2308 Antisense
CACGCTATGGCTCTTAACTTGACAC 622 195 2329 Antisense
GGGTAACAAGACATTCACAGACACT 252 497 2364 Antisense
GACACTGAACTTGAGGTTCTCACTA 496 347 2383 Antisense
GAGTCGACGACCGAGTGGTGGAATG 223 401 2416 Antisense
GAGGATCCCGTTGTGAAAGCTTCTG 592 403 2476 Antisense
GCAGAAAAGGCTCGTGACTTGGAAC 280 289 2506 Antisense
CTCCTCCGCCGTTTGAGAACGATAA 583 233 2696 Antisense
GAATCAGCATCGACTGAAGAGCCGA 655 325 2734 Antisense
Sequence Source Affymetrix Proprietary Database