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[189688_at] phosphodiesterase

Description T12B3.3 /REP_DB=WormBase Gene ID /WP=CE21178 /TR=Q9UAW8 /GB=AAD14735.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=phosphodiesterase
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 73      4grp_highest_8_8 > 31      4grp_high_8_8 > 05      6grp_highest_nonorm > 37      6grp_total > 73      4grp_total > 03      6grp_highest_sub > 03_04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6022
1 2 3 4 range
A 4506.8
(-1.8)
P 		3757.2
(-2.7)
P 		3468.2
(-3)
P 		8818.1
(3)
P 		5350
B 4606.8
(-1.9)
P 		3951.1
(-2.6)
P 		3539.5
(-3)
P 		9489.7
(3)
P 		5950
C 4595.4
(-2.1)
P 		3786.1
(-3)
P 		3789.2
(-3)
P 		9480.7
(3)
P 		5695
avr 4569.7
(-2) 		3831.5
(-2.8) 		3599
(-3) 		9262.8
(3) 		5664

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3113
1 2 3 4 5 6 range
A 4503.8
(-3)
P 		4894.9
(-2.2)
P 		7278.1
(3)
P 		5979.6
(0.2)
P 		5052.3
(-1.8)
P 		4757.5
(-2.5)
P 		2774
B 4736.4
(-3)
P 		5418.7
(0)
P 		5926.5
(2.3)
P 		6093.4
(3)
P 		5250.9
(-0.7)
P 		5986.3
(2.5)
P 		1357
C 4165.5
(-3)
P 		5166.1
(-0.3)
P 		5582.7
(0.9)
P 		6363.9
(3)
P 		5052.8
(-0.6)
P 		5316.2
(0.1)
P 		2198
avr 4468.6
(-3) 		5159.9
(-0.7) 		6262.4
(3) 		6145.6
(2.6) 		5118.7
(-0.8) 		5353.3
(-0) 		1794
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1207
1 2 3 4 range
A 1640.9
(3)
P 		815.6
(-3)
P 		906.7
(-2.3)
P 		1290.8
(0.5)
P 		825
B 1469.1
(0.2)
P 		975.8
(-2.2)
P 		820.2
(-3)
P 		2022.3
(3)
P 		1202
C 1521.2
(2.1)
P 		1088.5
(-3)
P 		1320.7
(-0.3)
P 		1598.6
(3)
P 		510
avr 1543.7
(2.2) 		960
(-3) 		1015.9
(-2.5) 		1637.2
(3) 		677

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1402
1 2 3 4 5 6 range
A 830.2
(-3)
P 		1740.9
(3)
P 		1585
(2)
P 		1516.6
(1.5)
P 		1524.6
(1.6)
P 		1158.3
(-0.8)
P 		911
B 1648
(-1.4)
P 		1803.7
(-0.2)
P 		1439.1
(-3)
P 		1613.9
(-1.7)
P 		2133.4
(2.3)
P 		2232.3
(3)
P 		793
C 1544
(0.2)
P 		1576.3
(0.6)
P 		1420.9
(-1.3)
P 		1279.8
(-3)
P 		1635
(1.3)
P 		1779.6
(3)
P 		500
avr 1340.7
(-3) 		1707
(2.2) 		1481.7
(-1) 		1470.1
(-1.2) 		1764.3
(3) 		1723.4
(2.4) 		424
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189688_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8886
Sequence Type Exemplar sequence
Representative Public ID T12B3.3 NCBI
Target Description T12B3.3 /REP_DB=WormBase Gene ID /WP=CE21178 /TR=Q9UAW8 /GB=AAD14735.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=phosphodiesterase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068775(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068775 NCBI Caenorhabditis elegans T12B3.3 (phosphodiesterase) mRNA, complete cds. 11/11 A 
SNAP00000023938 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7375156:7376523:1 11/11 None
GENEFINDER00000023944 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7375156:7376523:1 10/11 None
T12B3.3 ENSEMBL cdna:known chromosome:CEL140:IV:7375156:7376611:1 gene:T12B3.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7375161-7376529(+) 100.0 100.0
chrIV:7363969-7365076(-) 80.86 80.86
Public Domain and Genome References
Gene Title phosphodiesterase
Ensembl T12B3.3
Entrez Gene 177509 Entrez gene
SwissProt Q9UAW8 EMBL-EBI
WormBase CE21178 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604206_AT similar to membrane interacting protein of RGS16 cfa
CANINE:1585310_AT similar to membrane interacting protein of RGS16 cfa
CANINE_2:CFA.10052.1.S1_AT similar to membrane interacting protein of RGS16 cfa
CANINE_2:CFA.13044.1.A1_AT similar to membrane interacting protein of RGS16 cfa
CANINE_2:CFA.13044.1.A1_S_AT similar to membrane interacting protein of RGS16 cfa
CANINE_2:CFAAFFX.27702.1.S1_S_AT similar to membrane interacting protein of RGS16 cfa
CHICKEN:GGA.8868.1.S1_AT similar to membrane interacting protein of RGS16 gga
HU35KSUBA:RC_AA055048_AT membrane interacting protein of RGS16 hs
HU35KSUBA:N56415_S_AT membrane interacting protein of RGS16 hs
HU35KSUBC:RC_AA598405_AT membrane interacting protein of RGS16 hs
HU35KSUBD:RC_AA243501_AT membrane interacting protein of RGS16 hs
HG-U133A:202593_S_AT membrane interacting protein of RGS16 hs
HG-U133_PLUS_2:202593_S_AT membrane interacting protein of RGS16 hs
HG-FOCUS:202593_S_AT membrane interacting protein of RGS16 hs
HG-U133A_2:202593_S_AT membrane interacting protein of RGS16 hs
U133_X3P:G7706616_3P_A_AT membrane interacting protein of RGS16 hs
U133_X3P:HS.183684.1.A1_3P_AT membrane interacting protein of RGS16 hs
HG-U133_PLUS_2:226214_AT membrane interacting protein of RGS16 hs
HG-U133B:226214_AT membrane interacting protein of RGS16 hs
HG-U95AV2:41417_AT membrane interacting protein of RGS16 hs
HG-U95B:45490_S_AT membrane interacting protein of RGS16 hs
HG-U95C:65507_AT membrane interacting protein of RGS16 hs
MG-U74AV2:96905_AT membrane interacting protein of RGS16 mm
MOE430A:1418444_A_AT membrane interacting protein of RGS16 mm
MOUSE430A_2:1418444_A_AT membrane interacting protein of RGS16 mm
MOUSE430_2:1418444_A_AT membrane interacting protein of RGS16 mm
MU19KSUBA:TC20742_AT Membrane interacting protein of RGS16, mRNA (cDNA clone MGC:6768 IMAGE:3601420) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6071 glycerol metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8889 glycerophosphodiester phosphodiesterase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAD14735 Hypothetical protein T12B3.3 [Caenorhabditis elegans] ref|NP_501176.1| T12B3.3 [Caenorhabditis elegans] 0.0
blast CAE61869 Hypothetical protein CBG05849 [Caenorhabditis briggsae] 1.0E-176
blast CAE61869 Hypothetical protein CBG05849 [Caenorhabditis briggsae] 1.0E-173
blast CAB01567 Hypothetical protein F55C5.2 [Caenorhabditis elegans] ref|NP_506077.1| F55C5.2 [Caenorhabditis elegans] 3.0E-37
Protein Domains
Database ID Description E-Value
Pfam IPR004129 EMBL-EBI Glycerophosphoryl diester phosphodiesterase 3.7E-88
Pfam IPR004129 EMBL-EBI Glycerophosphoryl diester phosphodiesterase 8.4E-89
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501176.1 1 13-35
T12B3.3 1 13-35
SNAP00000023938 1 13-35
GENEFINDER00000023944 1 13-35
Sequence
Target Sequence 
>C. ELEGANS:189688_AT
ggatatttctcaagccttgattatggatcaaagaagacgtggaatgcgtgttgccgtgtg
gactgtaaatgatatggctgaaatgcattggatgctcaaaactctcaatattccaattct
cactgattatccagagctcacgactcaagctgctcatttggaagaattgcagaagagaga
ctacatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATATTTCTCAAGCCTTGATTATG 197 511 867 Antisense
GACGTGGAATGCGTGTTGCCGTGTG 306 375 902 Antisense
TGCGTGTTGCCGTGTGGACTGTAAA 476 579 911 Antisense
GTGGACTGTAAATGATATGGCTGAA 630 489 924 Antisense
GCTCAAAACTCTCAATATTCCAATT 273 301 960 Antisense
AACTCTCAATATTCCAATTCTCACT 367 141 966 Antisense
ATTCCAATTCTCACTGATTATCCAG 441 7 976 Antisense
TCTCACTGATTATCCAGAGCTCACG 247 613 984 Antisense
GATTATCCAGAGCTCACGACTCAAG 99 431 991 Antisense
GAGCTCACGACTCAAGCTGCTCATT 461 387 1000 Antisense
AGAATTGCAGAAGAGAGACTACATG 395 75 1029 Antisense
Sequence Source Affymetrix Proprietary Database