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[189679_at] EGF-domain protein

Description F21A3.3 /REP_DB=WormBase Gene ID /WP=CE09517 /TR=O17829 /GB=CAB04157.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=EGF-domain protein
Cluster 6grp_high1_10_10 > 90      6grp_total > 92      4grp_total > 58      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 479
1 2 3 4 range
A 252
(-0.2)
M 		469.9
(3)
P 		453
(2.7)
P 		65
(-3)
A 		405
B 270.3
(-0.2)
P 		396.4
(1.4)
P 		526.6
(3)
P 		47.2
(-3)
A 		479
C 275.6
(-0.1)
P 		390.8
(1.8)
P 		469.3
(3)
P 		90.4
(-3)
A 		379
avr 266
(-0.1) 		419
(2.1) 		483
(3) 		67.5
(-3) 		416

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 363
1 2 3 4 5 6 range
A 136.5
(-3)
A 		320.8
(2.1)
P 		241.8
(-0.1)
P 		281.2
(1)
P 		354
(3)
P 		182.1
(-1.7)
A 		218
B 216.9
(-2.9)
P 		337.5
(-0.4)
P 		314.7
(-0.9)
M 		324.5
(-0.7)
P 		499.5
(3)
P 		214.2
(-3)
P 		285
C 211.8
(-3)
P 		277.1
(-1)
P 		299.6
(-0.3)
P 		363.1
(1.6)
P 		409.8
(3)
P 		211.4
(-3)
P 		198
avr 188.4
(-3) 		311.8
(0.2) 		285.4
(-0.5) 		322.9
(0.5) 		421.1
(3) 		202.6
(-2.6) 		233
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 91.8
(1.5)
M 		102
(2.1)
P 		118.4
(3)
P 		9.5
(-3)
A 		109
B 86.2
(1.1)
P 		97.9
(1.7)
P 		122
(3)
P 		10.1
(-3)
A 		112
C 91.2
(0.1)
P 		112.3
(0.9)
P 		163.6
(3)
P 		15.3
(-3)
A 		148
avr 89.7
(0.8) 		104.1
(1.5) 		134.7
(3) 		11.6
(-3) 		123

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 178
1 2 3 4 5 6 range
A 25.2
(-3)
A 		114.1
(3)
P 		52.7
(-1.1)
P 		71.3
(0.1)
P 		106.8
(2.5)
P 		44.3
(-1.7)
A 		89
B 75.5
(-3)
P 		112.3
(-1.3)
P 		76.4
(-3)
M 		86
(-2.5)
P 		203
(3)
P 		79.9
(-2.8)
P 		128
C 78.5
(-2.3)
P 		84.5
(-1.7)
P 		76.2
(-2.5)
P 		73
(-2.8)
P 		132.6
(3)
P 		70.8
(-3)
P 		62
avr 59.7
(-3) 		103.6
(-0) 		68.4
(-2.4) 		76.8
(-1.8) 		147.5
(3) 		65
(-2.6) 		88
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189679_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13038
Sequence Type Exemplar sequence
Representative Public ID F21A3.3 NCBI
Target Description F21A3.3 /REP_DB=WormBase Gene ID /WP=CE09517 /TR=O17829 /GB=CAB04157.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=EGF-domain protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074461(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074461 NCBI Caenorhabditis elegans F21A3.3 (F21A3.3) mRNA, complete cds. 11/11 None
SNAP00000007987 ENSEMBL cdna:SNAP chromosome:CEL140:V:15520476:15522131:1 11/11 None
GENEFINDER00000008002 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15520301:15521949:1 11/11 None
F21A3.3 ENSEMBL cdna:known chromosome:CEL140:V:15520301:15521949:1 gene:F21A3.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15520300-15521949(+) 100.0 100.0
Public Domain and Genome References
Gene Title EGF-domain protein
Ensembl F21A3.3
Entrez Gene 184748 Entrez gene
SwissProt O17829 EMBL-EBI
WormBase CE09517 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04157 Hypothetical protein F21A3.3 [Caenorhabditis elegans] ref|NP_506862.1| F21A3.3 [Caenorhabditis elegans] 1.0E-172
blast CAB04157 Hypothetical protein F21A3.3 [Caenorhabditis elegans] ref|NP_506862.1| F21A3.3 [Caenorhabditis elegans] 1.0E-170
blast CAE71619 Hypothetical protein CBG18583 [Caenorhabditis briggsae] 1.0E-149
blast CAE71619 Hypothetical protein CBG18583 [Caenorhabditis briggsae] 1.0E-147
blast CAE71619 Hypothetical protein CBG18583 [Caenorhabditis briggsae] 1.0E-145
blast CAB04157 Hypothetical protein F21A3.3 [Caenorhabditis elegans] ref|NP_506862.1| F21A3.3 [Caenorhabditis elegans] 1.0E-143
blast AAL13330 Male abnormal protein 7 [Caenorhabditis elegans] ref|NP_508174.1| Male ABnormal family member (mab-7) [Caenorhabditis elegans] 8.0E-27
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.0E-9
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.0E-9
Sequence
Target Sequence 
>C. ELEGANS:189679_AT
atttctccatttttcgctgacaactgtgctttgtcctgcggccaatgcaaaaataacgga
aagcagctggcgctcgctcttccaccaattctcgacaacattgagtggtttgtgggcaga
tgggagtcaaagacaagtgctcatcatagatttccggagccaatgtccggaccttacaag
gaaatcttggacgttcaaatttcggaagttccctcttttgacagaccgccggttaatatt
tcagtgcgagccgagacgcttgatgggaccgatgtccacgtggaattcggatttttgacg
tctaaacctttccacgaggatacgggttttgtggagttgaacaaaccggacgaaggggac
gatttggtgtcgattgagcttgtgactaatacaggtctaatgctcatcgaggaaggaact
gtgcgcggtacccaaatccgattggaaaccaaatataagaagggaatggctggagtgttc
cgagacgaaattgtcaagtcaaagagaatgttcaatttgataaatgccaactctttggaa
gaacgggttgtgatggttgatggccgtggagttaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTCTCCATTTTTCGCTGACAACT 165 17 280 Antisense
GCTGACAACTGTGCTTTGTCCTGCG 113 297 295 Antisense
TAACGGAAAGCAGCTGGCGCTCGCT 52 637 333 Antisense
GACAAGTGCTCATCATAGATTTCCG 185 365 411 Antisense
AGCCAATGTCCGGACCTTACAAGGA 450 83 437 Antisense
CAGACCGCCGGTTAATATTTCAGTG 177 197 501 Antisense
AGCCGAGACGCTTGATGGGACCGAT 418 81 528 Antisense
GACGTCTAAACCTTTCCACGAGGAT 409 377 576 Antisense
GAGGAAGGAACTGTGCGCGGTACCC 390 335 688 Antisense
GAATGGCTGGAGTGTTCCGAGACGA 283 333 743 Antisense
GATGGTTGATGGCCGTGGAGTTACA 93 407 831 Antisense
Sequence Source Affymetrix Proprietary Database