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[189674_at] glycosyl hydralase

Description F53F4.8 /REP_DB=WormBase Gene ID /WP=CE10968 /TR=Q20722 /GB=CAB01206.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=glycosyl hydralase
Cluster 6grp_total > 70      4grp_total > 36      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 102
(-1)
A 		165.7
(3)
A 		71
(-3)
A 		131.6
(0.8)
A 		95
B 123.7
(3)
A 		78.9
(0.2)
A 		27.8
(-3)
A 		81.3
(0.3)
A 		96
C 100.1
(-0.5)
A 		126.7
(3)
A 		81.3
(-3)
A 		89.4
(-1.9)
A 		45
avr 108.6
(1.6) 		123.8
(3) 		60
(-3) 		100.8
(0.8) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 100
1 2 3 4 5 6 range
A 12.5
(-3)
A 		23.4
(-2.3)
A 		90.7
(2)
A 		107
(3)
A 		27.7
(-2)
A 		59.7
(-0)
A 		95
B 32.5
(-3)
A 		61.8
(-0.5)
A 		84.7
(1.5)
A 		101.8
(3)
A 		63.2
(-0.3)
A 		52
(-1.3)
A 		69
C 49.8
(-0.4)
A 		107.4
(3)
A 		62.6
(0.3)
A 		84.1
(1.6)
A 		7.2
(-3)
A 		89.9
(2)
A 		100
avr 31.6
(-3) 		64.2
(-0) 		79.3
(1.3) 		97.6
(3) 		32.7
(-2.9) 		67.2
(0.2) 		66
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 37.1
(3)
A 		36
(2.6)
A 		18.6
(-3)
A 		19.3
(-2.8)
A 		19
B 39.4
(3)
A 		19.5
(-0.6)
A 		6.4
(-3)
A 		17.3
(-1)
A 		33
C 33.1
(2.1)
A 		36.4
(3)
A 		28.3
(0.7)
A 		15.1
(-3)
A 		21
avr 36.5
(3) 		30.6
(1.2) 		17.8
(-2.8) 		17.2
(-3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 2.3
(-3)
A 		8.3
(-1.5)
A 		19.8
(1.2)
A 		27.1
(3)
A 		8.4
(-1.5)
A 		14.5
(-0)
A 		25
B 11.3
(-3)
A 		20.6
(0.6)
A 		20.6
(0.6)
A 		27
(3)
A 		25.7
(2.5)
A 		19.4
(0.1)
A 		16
C 18.5
(0.2)
A 		32.8
(3)
A 		15.9
(-0.3)
A 		16.9
(-0.1)
A 		2.3
(-3)
A 		30.1
(2.5)
A 		31
avr 10.7
(-3) 		20.6
(1.6) 		18.8
(0.7) 		23.7
(3) 		12.1
(-2.4) 		21.3
(1.9) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189674_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14418
Sequence Type Exemplar sequence
Representative Public ID F53F4.8 NCBI
Target Description F53F4.8 /REP_DB=WormBase Gene ID /WP=CE10968 /TR=Q20722 /GB=CAB01206.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=glycosyl hydralase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073972(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073972 NCBI Caenorhabditis elegans F53F4.8 (F53F4.8) mRNA, complete cds. 11/11 None
SNAP00000012441 ENSEMBL cdna:SNAP chromosome:CEL140:V:13616828:13620315:-1 11/11 None
GENEFINDER00000012454 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13614009:13620315:-1 10/11 None
F53F4.8 ENSEMBL cdna:known chromosome:CEL140:V:13616828:13620315:-1 gene:F53F4.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13616827-13620315(-) 100.0 100.0
Public Domain and Genome References
Gene Title glycosyl hydralase
Ensembl F53F4.8
Entrez Gene 179848 Entrez gene
SwissProt Q20722 EMBL-EBI
WormBase CE10968 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CHICKEN:GGA.7598.1.S1_AT similar to maltase-glucoamylase gga
CHICKEN:GGAAFFX.124.1.S1_AT similar to maltase-glucoamylase gga
CHICKEN:GGAAFFX.124.1.S1_S_AT similar to maltase-glucoamylase gga
CHICKEN:GGAAFFX.124.2.S1_AT similar to maltase-glucoamylase gga
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4553 hydrolase activity, hydrolyzing O-glycosyl compounds inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01206 Hypothetical protein F53F4.8 [Caenorhabditis elegans] ref|NP_506373.1| F53F4.8 [Caenorhabditis elegans] 0.0
blast CAE66278 Hypothetical protein CBG11523 [Caenorhabditis briggsae] 0.0
blast CAE59001 Hypothetical protein CBG02276 [Caenorhabditis briggsae] 0.0
blast NP_494897 R05F9.12 [Caenorhabditis elegans] gb|AAA83174.3| Hypothetical protein R05F9.12 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000519 EMBL-EBI P-type trefoil domain 1.4E-6
Pfam IPR000322 EMBL-EBI Glycoside hydrolase, family 31 1.0E-126
Pfam IPR000322 EMBL-EBI Glycoside hydrolase, family 31 1.0E-126
Pfam IPR000322 EMBL-EBI Glycoside hydrolase, family 31 1.0E-126
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000012454 2 1133-1155,1176-1195
Sequence
Target Sequence 
>C. ELEGANS:189674_AT
gtattcttgtggctcctgtagtttatcaaggagcagtttccacaagcctttatttaccaa
atgatgtatggtactcacttttcaattatatgtatggaagtagaatcgacccaggattca
taactgttccatctccaaccacttctagaattccagtttttgttagaggaggctatgtca
ttccacgccaaactccgactacaacaacaacaatgagccgattgaaccccttcgagctcc
tgattgcaccttgccaacttggtagagctgcgggagttctgtactgggatgatggtcaga
ggattgtggactcatttgatacccatgactatcatcagttcgattttaagttcaactcta
cgattaacggtgcttcgttggccattacaagaactagaaagggaacaactgttttaccta
cattggatattttggaaatctttaactacacaacacctccgaacttccgatcgttccgtt
tgaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTATTCTTGTGGCTCCTGTAGTTTA 182 451 1967 Antisense
AGGAGCAGTTTCCACAAGCCTTTAT 73 57 1995 Antisense
GATGTATGGTACTCACTTTTCAATT 339 409 2029 Antisense
AGGATTCATAACTGTTCCATCTCCA 569 57 2079 Antisense
CCATCTCCAACCACTTCTAGAATTC 173 271 2095 Antisense
AGAGGAGGCTATGTCATTCCACGCC 342 67 2131 Antisense
GATTGCACCTTGCCAACTTGGTAGA 200 433 2208 Antisense
GTAGAGCTGCGGGAGTTCTGTACTG 184 457 2228 Antisense
GGTGCTTCGTTGGCCATTACAAGAA 35 501 2335 Antisense
GAAATCTTTAACTACACAACACCTC 356 361 2401 Antisense
GAACTTCCGATCGTTCCGTTTGAAT 202 349 2427 Antisense
Sequence Source Affymetrix Proprietary Database