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[189660_at] aspartyl protease

Description F59D6.3 /REP_DB=WormBase Gene ID /WP=CE11480 /TR=O16338 /GB=AAB65878.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=aspartyl protease
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 05      6grp_highest_nonorm > 60      6grp_total > 04      4grp_total > 54      6grp_highest_sub > De      6grp_high1_sub > De      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 920
1 2 3 4 range
A 931.2
(3)
P 		403.3
(-0.5)
P 		420.5
(-0.4)
P 		20.8
(-3)
A 		910
B 766.7
(3)
P 		339.3
(-0.4)
P 		310.9
(-0.6)
P 		11
(-3)
A 		756
C 642.5
(3)
P 		361.8
(-0.1)
P 		360.2
(-0.1)
P 		92.7
(-3)
A 		550
avr 780.1
(3) 		368.1
(-0.3) 		363.9
(-0.4) 		41.5
(-3) 		739

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 572
1 2 3 4 5 6 range
A 725.7
(3)
P 		555.7
(1.2)
P 		392.6
(-0.5)
P 		282
(-1.7)
P 		346
(-1)
P 		153.6
(-3)
P 		572
B 629.2
(3)
P 		471.8
(1)
P 		363.5
(-0.4)
P 		335
(-0.7)
P 		303.2
(-1.1)
P 		154.2
(-3)
P 		475
C 601.9
(3)
P 		393
(0.1)
P 		320.4
(-0.9)
P 		342
(-0.6)
P 		337
(-0.7)
P 		172.5
(-3)
P 		429
avr 652.3
(3) 		473.5
(0.8) 		358.8
(-0.6) 		319.7
(-1.1) 		328.7
(-0.9) 		160.1
(-3) 		492
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 337
1 2 3 4 range
A 339
(3)
P 		87.5
(-1.5)
P 		109.9
(-1.1)
P 		3
(-3)
A 		336
B 244.5
(3)
P 		83.8
(-1)
P 		72
(-1.3)
P 		2.4
(-3)
A 		242
C 212.7
(3)
P 		104
(-0.3)
P 		125.5
(0.3)
P 		15.6
(-3)
A 		197
avr 265.4
(3) 		91.8
(-1) 		102.5
(-0.8) 		7
(-3) 		258

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 186
1 2 3 4 5 6 range
A 133.8
(0.6)
P 		197.6
(3)
P 		85.5
(-1.2)
P 		71.5
(-1.7)
P 		104.4
(-0.5)
P 		37.4
(-3)
P 		160
B 218.9
(3)
P 		157.1
(0.7)
P 		88.3
(-1.9)
P 		88.7
(-1.8)
P 		123.2
(-0.6)
P 		57.5
(-3)
P 		161
C 223.1
(3)
P 		119.9
(-0.7)
P 		81.6
(-2.1)
P 		68.8
(-2.6)
P 		109.1
(-1.1)
P 		57.7
(-3)
P 		165
avr 191.9
(3) 		158.2
(1.6) 		85.1
(-1.5) 		76.3
(-1.9) 		112.2
(-0.4) 		50.9
(-3) 		141
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189660_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15717
Sequence Type Exemplar sequence
Representative Public ID F59D6.3 NCBI
Target Description F59D6.3 /REP_DB=WormBase Gene ID /WP=CE11480 /TR=O16338 /GB=AAB65878.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=aspartyl protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071424(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071424 NCBI Caenorhabditis elegans F59D6.3 (F59D6.3) mRNA, complete cds. 11/11 None
SNAP00000018888 ENSEMBL cdna:SNAP chromosome:CEL140:V:3024390:3027713:1 11/11 None
GENEFINDER00000018895 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3023357:3027877:1 11/11 None
F59D6.3 ENSEMBL cdna:known chromosome:CEL140:V:3024046:3027713:1 gene:F59D6.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3024045-3027713(+) 100.0 100.0
Public Domain and Genome References
Gene Title aspartyl protease
Ensembl F59D6.3
Entrez Gene 178750 Entrez gene
SwissProt O16338 EMBL-EBI
WormBase CE11480 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4194 pepsin A activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB65878 Hypothetical protein F59D6.3 [Caenorhabditis elegans] ref|NP_503825.1| F59D6.3 [Caenorhabditis elegans] 0.0
blast AAB65877 Hypothetical protein F59D6.2 [Caenorhabditis elegans] ref|NP_503826.2| F59D6.2 [Caenorhabditis elegans] 1.0E-178
blast AAB65877 Hypothetical protein F59D6.2 [Caenorhabditis elegans] ref|NP_503826.2| F59D6.2 [Caenorhabditis elegans] 1.0E-168
blast CAE72985 Hypothetical protein CBG20329 [Caenorhabditis briggsae] 1.0E-105
Protein Domains
Database ID Description E-Value
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 9.4E-27
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 6.3E-26
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 9.4E-27
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 6.3E-26
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 9.4E-27
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 3.8E-16
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503825.1 1 20-42
F59D6.3 1 20-42
Sequence
Target Sequence 
>C. ELEGANS:189660_AT
tggccggctactgcacttaatggaactagtacaacaatgcaaaacttactgccacaactt
gatcagaaactcttcacgacttatttcacaaaatccaacatgcataacggaacagcagga
ggtgacattatgtttggagcaattgacaccacacattgccaatcacaagtcaattacgtc
ccacttgcctacaactcgttctggagttactctgtggatggattttcaatcggcacttat
tcaagaactcaaacagaaacgaccatccccgacacttcatccggctggactggtgttcca
aacgttgtccttgccggaatcgtaaaagccactggagcaacatacgactggaatcatcaa
gcatacactctcccgtgctcttcaacagccacactacctgacatggttttcacaatcggt
ggaaactcgtacaacgtcagagcagtagaatatgtggtcaacttgaacttgccaaacgga
cagtgtgctctttctctgttcggaacagccgcctcacaatctggaccggcgtggatcctt
ggcgacaacttccttagaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGCCGGCTACTGCACTTAATGGAA 589 517 811 Antisense
AAAACTTACTGCCACAACTTGATCA 114 131 851 Antisense
AACTCTTCACGACTTATTTCACAAA 250 141 878 Antisense
CAATCACAAGTCAATTACGTCCCAC 679 179 970 Antisense
ACTTGCCTACAACTCGTTCTGGAGT 376 103 993 Antisense
CTCAAACAGAAACGACCATCCCCGA 345 227 1058 Antisense
CACTTCATCCGGCTGGACTGGTGTT 158 197 1083 Antisense
AACAGCCACACTACCTGACATGGTT 587 135 1194 Antisense
TTCTCTGTTCGGAACAGCCGCCTCA 587 689 1302 Antisense
TCACAATCTGGACCGGCGTGGATCC 287 627 1324 Antisense
TCCTTGGCGACAACTTCCTTAGAAG 703 591 1346 Antisense
Sequence Source Affymetrix Proprietary Database