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[189658_at] spe-12

Description T02E1.1 /REP_DB=WormBase Gene ID /WP=CE13046 /GEN=spe-12 /TR=Q17387 /GB=CAB04665.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=spe-12
Cluster 6grp_total > 86      4grp_total > 25      6grp_cgc > 68      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 4
(-3)
A 		45.7
(1.6)
A 		25.4
(-0.6)
A 		58.5
(3)
A 		55
B 4.8
(-3)
A 		6.3
(-2.7)
A 		34.4
(2.2)
A 		38.7
(3)
A 		34
C 10.1
(-2.9)
A 		43
(1.3)
A 		9.1
(-3)
A 		56.6
(3)
A 		48
avr 6.3
(-3) 		31.7
(0.4) 		23
(-0.8) 		51.3
(3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 5.1
(-3)
A 		11.2
(-2.5)
A 		12
(-2.5)
A 		29.2
(-1.2)
A 		22.1
(-1.7)
A 		86.3
(3)
A 		81
B 3.3
(-3)
A 		11.3
(-2.2)
A 		14.8
(-1.8)
A 		4.7
(-2.9)
A 		62.5
(3)
A 		53.2
(2.1)
A 		59
C 29.5
(-1.5)
A 		26.6
(-1.8)
A 		11.8
(-3)
A 		61.3
(1.1)
A 		48.2
(0)
A 		83.5
(3)
A 		72
avr 12.6
(-3) 		16.4
(-2.6) 		12.9
(-3) 		31.7
(-1.1) 		44.3
(0.1) 		74.3
(3) 		62
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 1.5
(-3)
A 		9.9
(3)
A 		6.6
(0.6)
A 		8.6
(2.1)
A 		8
B 1.5
(-3)
A 		1.6
(-2.9)
A 		8
(2.8)
A 		8.2
(3)
A 		7
C 3.3
(-2.9)
A 		12.3
(3)
A 		3.2
(-3)
A 		9.5
(1.2)
A 		9
avr 2.1
(-3) 		7.9
(2.2) 		5.9
(0.4) 		8.8
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 0.9
(-3)
A 		4
(-2.1)
A 		2.6
(-2.5)
A 		7.4
(-1.1)
A 		6.7
(-1.3)
A 		21
(3)
A 		20
B 1.2
(-3)
A 		3.7
(-2.4)
A 		3.6
(-2.4)
A 		1.3
(-3)
A 		25.4
(3)
A 		19.8
(1.6)
A 		24
C 10.9
(-1.1)
A 		8.1
(-1.8)
A 		3
(-3)
A 		12.3
(-0.8)
A 		15.6
(0)
A 		27.9
(3)
A 		25
avr 4.3
(-2.6) 		5.3
(-2.3) 		3.1
(-3) 		7
(-1.8) 		15.9
(0.9) 		22.9
(3) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189658_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2176
Sequence Type Exemplar sequence
Representative Public ID T02E1.1 NCBI
Target Description T02E1.1 /REP_DB=WormBase Gene ID /WP=CE13046 /GEN=spe-12 /TR=Q17387 /GB=CAB04665.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=spe-12
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059841(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059841 NCBI Caenorhabditis elegans defective SPErmatogenesis family member (spe-12) (spe-12) mRNA, complete cds. 11/11 None
SNAP00000000257 ENSEMBL cdna:SNAP chromosome:CEL140:I:8232733:8234270:-1 9/11 None
T02E1.1 ENSEMBL cdna:known chromosome:CEL140:I:8232733:8234384:-1 gene:T02E1.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000000263 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8232663-8234315(-) 100.0 100.0
Public Domain and Genome References
Gene Title spe-12
Gene Symbol spe-12
Ensembl T02E1.1
Entrez Gene 172606 Entrez gene
SwissProt Q17387 EMBL-EBI
WormBase CE13046 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04665 Hypothetical protein T02E1.1 [Caenorhabditis elegans] ref|NP_492242.1| defective SPErmatogenesis family member (spe-12) [Caenorhabditis elegans] gb|AAB02238.1| SPE-12 1.0E-150
blast CAB04665 Hypothetical protein T02E1.1 [Caenorhabditis elegans] ref|NP_492242.1| defective SPErmatogenesis family member (spe-12) [Caenorhabditis elegans] gb|AAB02238.1| SPE-12 1.0E-111
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492242.1 1 200-222
T02E1.1 1 200-222
SNAP00000000257 1 184-206
Sequence
Target Sequence 
>C. ELEGANS:189658_AT
acaccgaatgtcatactcgagccccaagttgcggttttcaaatgatgccgattatgatta
atggattgaatacgtccaacaaagttgtacgctttggatgctttccaactcgagatattt
tgggaaatcagttttttctaaacaagcgatattactcgaattcttttgatggaatataca
gtatgtttgactactttgcattcgagaatactggtattgctgattatttaatttattaca
aaccgacagcggaagaactaaaagcagttagatatgttcatgaaaacagatgggttgaaa
atccacaggaattcatagtccagaagaaaaatgagagtagcgcagaagctcattcggatg
gtctcaatgcatacttccgccataaaaagattaccccactcatgataatttacattctat
tcatttgcttttctgtcattttcaagcttatctggcttgtacatacagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACACCGAATGTCATACTCGAGCCCC 535 109 221 Antisense
GAGCCCCAAGTTGCGGTTTTCAAAT 626 381 239 Antisense
AGTTGTACGCTTTGGATGCTTTCCA 410 61 303 Antisense
GCGATATTACTCGAATTCTTTTGAT 436 291 366 Antisense
GTTTGACTACTTTGCATTCGAGAAT 602 449 405 Antisense
GCAGAAGCTCATTCGGATGGTCTCA 562 313 562 Antisense
GGATGGTCTCAATGCATACTTCCGC 389 515 576 Antisense
TAAAAAGATTACCCCACTCATGATA 614 639 603 Antisense
TTTACATTCTATTCATTTGCTTTTC 643 673 629 Antisense
CATTTGCTTTTCTGTCATTTTCAAG 411 215 642 Antisense
AGCTTATCTGGCTTGTACATACAGC 638 77 665 Antisense
Sequence Source Affymetrix Proprietary Database