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[189653_s_at]

Description R08E5.2 /REP_DB=WormBase Gene ID /WP=CE12574 /TR=O01592 /GB=AAB52276.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cysteine synthase
Cluster 6grp_highest_10_10 > 15      6grp_high1_10_10 > 41      4grp_highest_8_8 > 05      4grp_high_8_8 > 30      6grp_highest_nonorm > 52      6grp_total > 41      4grp_total > 24      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 732
1 2 3 4 range
A 694.4
(-3)
P 		1184.4
(2.5)
P 		1030.9
(0.8)
P 		1229.5
(3)
P 		535
B 857.7
(-3)
P 		966.9
(-1.8)
P 		1155.4
(0.1)
P 		1426.5
(3)
P 		569
C 726.2
(-3)
P 		953.8
(1)
P 		1048.3
(2.6)
P 		1068.7
(3)
P 		343
avr 759.4
(-3) 		1035
(0.4) 		1078.2
(1) 		1241.6
(3) 		482

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 775
1 2 3 4 5 6 range
A 635.4
(0.1)
P 		776.6
(1.9)
P 		859.5
(3)
P 		700.1
(0.9)
P 		488.3
(-1.8)
P 		393.2
(-3)
P 		466
B 717.8
(-0.2)
P 		860.4
(0.9)
P 		1133.2
(3)
P 		816.1
(0.5)
P 		576.4
(-1.3)
P 		358.7
(-3)
P 		775
C 653.2
(-1)
P 		769.3
(0.5)
P 		971.1
(3)
P 		826.2
(1.2)
P 		506.3
(-2.8)
P 		492.7
(-3)
P 		478
avr 668.8
(-0.3) 		802.1
(1.1) 		987.9
(3) 		780.8
(0.8) 		523.7
(-1.9) 		414.9
(-3) 		573
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 185
1 2 3 4 range
A 252.8
(1.9)
P 		257.1
(2.2)
P 		269.5
(3)
P 		180
(-3)
P 		90
B 273.5
(0.2)
P 		238.8
(-3)
P 		267.7
(-0.3)
P 		304
(3)
P 		65
C 240.4
(-1)
P 		274.2
(0)
P 		365.4
(3)
P 		180.2
(-3)
P 		185
avr 255.6
(-0.4) 		256.7
(-0.3) 		300.9
(3) 		221.4
(-3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 191
1 2 3 4 5 6 range
A 117.1
(-2.3)
P 		276.2
(3)
P 		187.2
(0)
P 		177.6
(-0.3)
P 		147.3
(-1.3)
P 		95.7
(-3)
P 		181
B 249.8
(1.6)
P 		286.4
(3)
P 		275.2
(2.6)
P 		216.2
(0.2)
P 		234.2
(0.9)
P 		133.8
(-3)
P 		153
C 242.1
(2.6)
P 		234.7
(2.1)
P 		247.2
(3)
P 		166.2
(-2.8)
P 		163.8
(-3)
P 		164.9
(-2.9)
P 		83
avr 203
(0.2) 		265.8
(3) 		236.5
(1.7) 		186.7
(-0.5) 		181.8
(-0.8) 		131.5
(-3) 		134
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189653_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15021
Sequence Type Exemplar sequence
Representative Public ID R08E5.2 NCBI
Target Description R08E5.2 /REP_DB=WormBase Gene ID /WP=CE12574 /TR=O01592 /GB=AAB52276.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cysteine synthase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071645(11), NM_182331(11), NM_182332(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071645 NCBI Caenorhabditis elegans R08E5.2a (R08E5.2) mRNA, complete cds. 11/11 None
NM_182331 NCBI Caenorhabditis elegans R08E5.2b (R08E5.2) mRNA, complete cds. 11/11 None
NM_182332 NCBI Caenorhabditis elegans R08E5.2c (R08E5.2) mRNA, complete cds. 11/11 None
SNAP00000044453 ENSEMBL cdna:SNAP chromosome:CEL140:V:3774411:3775947:-1 11/11 None
GENEFINDER00000044454 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3774411:3775947:-1 11/11 None
R08E5.2a ENSEMBL cdna:known chromosome:CEL140:V:3774361:3775947:-1 gene:R08E5.2 11/11 None
R08E5.2b.1 ENSEMBL cdna:known chromosome:CEL140:V:3774411:3775950:-1 gene:R08E5.2 11/11 None
R08E5.2b.2 ENSEMBL cdna:known chromosome:CEL140:V:3774411:3775923:-1 gene:R08E5.2 11/11 None
R08E5.2b.3 ENSEMBL cdna:known chromosome:CEL140:V:3774411:3775934:-1 gene:R08E5.2 11/11 None
R08E5.2b.4 ENSEMBL cdna:known chromosome:CEL140:V:3774411:3775927:-1 gene:R08E5.2 11/11 None
R08E5.2c ENSEMBL cdna:known chromosome:CEL140:V:3774415:3775949:-1 gene:R08E5.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3774410-3775947(-) 100.0 100.0
Public Domain and Genome References
Ensembl R08E5.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB52276 Hypothetical protein R08E5.2a [Caenorhabditis elegans] ref|NP_504046.1| R08E5.2a [Caenorhabditis elegans] 1.0E-180
blast CAE65468 Hypothetical protein CBG10434 [Caenorhabditis briggsae] 1.0E-162
blast AAO26010 Hypothetical protein R08E5.2c [Caenorhabditis elegans] ref|NP_872132.1| R08E5.2c [Caenorhabditis elegans] 1.0E-161
Protein Domains
Database ID Description E-Value
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 1.2E-124
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 7.9E-79
Sequence
Target Sequence 
>C. ELEGANS:189653_S_AT
aagcttccaaatgctcattgccttgatcaattctcgaatcccgccaatccagccgctcat
tatcgtaccactgggccagaaatttggaggcaaaccgaaggaaaagttgatattgtgtgc
tttggtgtaggatcgagtggaactgtgacaggagtcggaagatacctccgcgagcagaat
ccgaatattgagatctacccggttgagccatacgagagttcagtcttgagtgggctgcca
agagggccacacaaaattcaaggaatcggtgccggcattgtgccaggaaacgtcgatcgc
tcgttgttcacggaaattttgcgcatcaagtctgacgatgcgatgcaaatggcgcggcga
c

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGCTTCCAAATGCTCATTGCCTTG 172 151 427 Antisense
GCCTTGATCAATTCTCGAATCCCGC 269 281 446 Antisense
CTCATTATCGTACCACTGGGCCAGA 569 227 482 Antisense
TACCTCCGCGAGCAGAATCCGAATA 633 649 589 Antisense
AGATCTACCCGGTTGAGCCATACGA 199 65 617 Antisense
GAGTTCAGTCTTGAGTGGGCTGCCA 639 397 642 Antisense
AAGGAATCGGTGCCGGCATTGTGCC 295 161 686 Antisense
TTGTGCCAGGAAACGTCGATCGCTC 342 709 704 Antisense
ATCGCTCGTTGTTCACGGAAATTTT 284 37 722 Antisense
AATTTTGCGCATCAAGTCTGACGAT 320 175 741 Antisense
GATGCGATGCAAATGGCGCGGCGAC 620 413 763 Antisense
Sequence Source Affymetrix Proprietary Database