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[189651_s_at] Methyltransferase

Description C27F2.4 /REP_DB=WormBase Gene ID /WP=CE01171 /TR=Q18257 /GB=AAA81422.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Methyltransferase
Cluster 6grp_highest_10_10 > 65      6grp_high1_10_10 > 56      4grp_highest_8_8 > 23      4grp_high_8_8 > 05      6grp_highest_nonorm > 83      6grp_total > 57      4grp_total > 03      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 950
1 2 3 4 range
A 555.8
(-2.3)
P 		511.3
(-2.6)
P 		461.9
(-3)
P 		1271.9
(3)
P 		810
B 531.5
(-2)
P 		386.2
(-3)
P 		379.2
(-3)
P 		1273.4
(3)
P 		894
C 502.4
(-2.6)
P 		450.8
(-3)
P 		463.7
(-2.9)
P 		1328.9
(3)
P 		878
avr 529.9
(-2.3) 		449.4
(-2.9) 		434.9
(-3) 		1291.4
(3) 		857

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 282
1 2 3 4 5 6 range
A 475.1
(-0.1)
P 		409.6
(-3)
P 		543.6
(3)
P 		490.8
(0.6)
P 		485.7
(0.4)
P 		455.6
(-0.9)
P 		134
B 522
(-0.8)
P 		533.2
(-0.5)
P 		459.6
(-2.6)
P 		482
(-1.9)
P 		444.3
(-3)
P 		654.8
(3)
P 		211
C 433.7
(-1.6)
P 		553.4
(1.1)
P 		401
(-2.4)
P 		532.7
(0.6)
P 		373.3
(-3)
P 		636.7
(3)
P 		263
avr 476.9
(-1.3) 		498.7
(-0.4) 		468.1
(-1.6) 		501.8
(-0.3) 		434.4
(-3) 		582.4
(3) 		148
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 202.4
(3)
P 		111
(-3)
P 		120.8
(-2.4)
P 		186.2
(1.9)
P 		91
B 169.5
(-0.3)
P 		95.4
(-2.8)
P 		87.9
(-3)
P 		271.4
(3)
P 		184
C 166.3
(-0.7)
P 		129.6
(-3)
P 		161.6
(-1)
P 		224.1
(3)
P 		95
avr 179.4
(0.5) 		112
(-3) 		123.4
(-2.4) 		227.2
(3) 		115

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 157
1 2 3 4 5 6 range
A 87.6
(-3)
P 		145.7
(2.9)
P 		118.4
(0.1)
P 		124.5
(0.8)
P 		146.6
(3)
P 		110.9
(-0.6)
P 		59
B 181.6
(0.2)
P 		177.5
(-0)
P 		111.6
(-3)
P 		127.7
(-2.3)
P 		180.5
(0.1)
P 		244.2
(3)
P 		133
C 160.8
(0.2)
P 		168.8
(0.6)
P 		102.1
(-3)
P 		107.1
(-2.7)
P 		120.8
(-2)
P 		213.1
(3)
P 		111
avr 143.3
(-0.5) 		164
(1.1) 		110.7
(-3) 		119.8
(-2.3) 		149.3
(-0.1) 		189.4
(3) 		79
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189651_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6508
Sequence Type Exemplar sequence
Representative Public ID C27F2.4 NCBI
Target Description C27F2.4 /REP_DB=WormBase Gene ID /WP=CE01171 /TR=Q18257 /GB=AAA81422.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Methyltransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065650(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065650 NCBI Caenorhabditis elegans C27F2.4 (Methyltransferase) mRNA, complete cds. 11/11 None
SNAP00000001012 ENSEMBL cdna:SNAP chromosome:CEL140:III:4958740:4960255:-1 11/11 None
GENEFINDER00000001025 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4958740:4960255:-1 11/11 None
C27F2.4 ENSEMBL cdna:known chromosome:CEL140:III:4958497:4960261:-1 gene:C27F2.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4958739-4960255(-) 100.0 100.0
Public Domain and Genome References
Gene Title Methyltransferase
Ensembl C27F2.4
Entrez Gene 175671 Entrez gene
SwissProt Q18257 EMBL-EBI
WormBase CE01171 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:247926_AT expressed protein at
CANINE:1585140_AT similar to Putative methyltransferase HUSSY-03 (Williams-Beuren syndrome chromosome region 22 protein homolog) cfa
CANINE_2:CFA.10777.1.S1_AT similar to Putative methyltransferase HUSSY-03 (Williams-Beuren syndrome chromosome region 22 protein homolog) cfa
CANINE_2:CFAAFFX.20042.1.S1_AT similar to Putative methyltransferase HUSSY-03 (Williams-Beuren syndrome chromosome region 22 protein homolog) cfa
CANINE_2:CFAAFFX.20043.1.S1_S_AT similar to Putative methyltransferase HUSSY-03 (Williams-Beuren syndrome chromosome region 22 protein homolog) cfa
DROSOPHILA_2:1633751_AT dm
DROSGENOME1:149471_AT dm
CHICKEN:GGA.15895.1.S1_AT similar to Williams Beuren syndrome chromosome region 22 protein; Williams-Beuren candidate region putative methyltransferase gga
HG-U95AV2:40089_AT Williams Beuren syndrome chromosome region 22 hs
HG-U133_PLUS_2:207628_S_AT Williams Beuren syndrome chromosome region 22 hs
HG-U133A:207628_S_AT Williams Beuren syndrome chromosome region 22 hs
HG-FOCUS:207628_S_AT Williams Beuren syndrome chromosome region 22 hs
HG-U133A_2:207628_S_AT Williams Beuren syndrome chromosome region 22 hs
HU35KSUBA:AA115397_AT Williams Beuren syndrome chromosome region 22 hs
HU35KSUBC:RC_AA455304_AT Williams Beuren syndrome chromosome region 22 hs
HU35KSUBD:RC_AA521478_AT Williams Beuren syndrome chromosome region 22 hs
U133_X3P:G8923713_3P_A_AT Williams Beuren syndrome chromosome region 22 hs
U133_X3P:HS.89143.0.S1_3P_AT Williams Beuren syndrome chromosome region 22 hs
HG-U133_PLUS_2:231415_AT Williams Beuren syndrome chromosome region 22 hs
HG-U133B:231415_AT Williams Beuren syndrome chromosome region 22 hs
HG-U95AV2:40090_AT Williams Beuren syndrome chromosome region 22 hs
HG-U95C:62984_R_AT Williams Beuren syndrome chromosome region 22 hs
MG-U74BV2:111712_AT Williams Beuren syndrome chromosome region 22 mm
MG-U74BV2:109160_AT Williams Beuren syndrome chromosome region 22 mm
MOE430A:1423575_A_AT Williams Beuren syndrome chromosome region 22 mm
MOUSE430A_2:1423575_A_AT Williams Beuren syndrome chromosome region 22 mm
MOUSE430_2:1423575_A_AT Williams Beuren syndrome chromosome region 22 mm
MU19KSUBA:TC15899_AT Williams-Beuren syndrome critical region protein 22 (Wbscr22) mm
RG-U34B:RC_AI071935_AT similar to Putative methyltransferase HUSSY-03 (Williams-Beuren syndrome chromosome region 22 protein homolog) rn
RAE230A:1389450_AT similar to Putative methyltransferase HUSSY-03 (Williams-Beuren syndrome chromosome region 22 protein homolog) rn
RAT230_2:1389450_AT similar to Putative methyltransferase HUSSY-03 (Williams-Beuren syndrome chromosome region 22 protein homolog) rn
RAE230B:1382641_AT Similar to Putative methyltransferase HUSSY-03 (Williams-Beuren syndrome chromosome region 22 protein homolog) rn
RAT230_2:1382641_AT Similar to Putative methyltransferase HUSSY-03 (Williams-Beuren syndrome chromosome region 22 protein homolog) rn
RG-U34B:RC_AA850982_AT Similar to Putative methyltransferase HUSSY-03 (Williams-Beuren syndrome chromosome region 22 protein homolog) rn
YEAST_2:1779254_AT Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Sc
Protein Similarities
Method ID Description E-Value
blast NP_498051 C27F2.4 [Caenorhabditis elegans] gb|AAA81422.1| Hypothetical protein C27F2.4 [Caenorhabditis elegans] 1.0E-154
blast CAE71311 Hypothetical protein CBG18202 [Caenorhabditis briggsae] 1.0E-151
blast AAF54183 CG10903-PA [Drosophila melanogaster] gb|AAL48794.1| RE22146p [Drosophila melanogaster] ref|NP_649762.1| CG10903-PA [Drosophila melanogaster] 3.0E-70
Sequence
Target Sequence 
>C. ELEGANS:189651_S_AT
tgttggcgttgacgtttccagaccaatgttagaaattgctcgtcaagatgaagaccttga
atctggagatttcattcatcaagatatgggactaggaatgccattcaggcctggaagttt
tgatggtgcaattagtatctcagccattcaatggttatgccatgcaaatgcttcagatga
gaatccacgtaaacgtcttctcttcttttttcaatcactctatggatgccttgggcgtgg
atcacgagcagttttccaattctacccggaaaacgatgagcaatgcgatctgattatggg
tcaagctcataaagcaggattcaatggaggacttgttgttgattttcctgaagccgccaa
acggaagaaagtttatttggttttgatgactggaggagtggttcaacttccacaagcatt
gactgaagatggagaagaaagcagaacacagattgataatgctggaagaagatttgtgtg
gaactctcgtaaaaatgagaaagtcgcgaaaggatcgaaagcatggattgaggcgaaacg
tcaacgacagattaagcagggacgcgacgtcagacacgagtcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTTGGCGTTGACGTTTCCAGACCA 505 561 246 Antisense
GGAATGCCATTCAGGCCTGGAAGTT 494 529 340 Antisense
GGTGCAATTAGTATCTCAGCCATTC 515 501 370 Antisense
TCCACGTAAACGTCTTCTCTTCTTT 248 587 429 Antisense
TCTTCTTTTTTCAATCACTCTATGG 405 617 446 Antisense
ATCACTCTATGGATGCCTTGGGCGT 332 27 459 Antisense
GATGCCTTGGGCGTGGATCACGAGC 379 415 470 Antisense
TCACGAGCAGTTTTCCAATTCTACC 375 625 487 Antisense
TTGTTGATTTTCCTGAAGCCGCCAA 6 707 581 Antisense
GTGGTTCAACTTCCACAAGCATTGA 511 489 643 Antisense
GGGACGCGACGTCAGACACGAGTCG 8 495 804 Antisense
Sequence Source Affymetrix Proprietary Database