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[189643_at] heat shock protein

Description F08H9.3 /REP_DB=WormBase Gene ID /WP=CE09267 /TR=Q19227 /GB=CAB01146.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=heat shock protein
Cluster 6grp_highest_10_10 > 18      6grp_high1_10_10 > 80      6grp_highest_nonorm > 90      6grp_total > 82      4grp_total > 02      6grp_highest_sub > 04_05      6grp_high1_sub > 02_03_04_05      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 852
1 2 3 4 range
A 38.9
(-2.8)
A 		39.6
(-2.8)
A 		5.3
(-3)
A 		855
(3)
P 		850
B 15.6
(-2.9)
A 		87.6
(-2.3)
A 		3
(-3)
A 		729
(3)
P 		726
C 43.3
(-3)
A 		40.9
(-3)
A 		37.7
(-3)
A 		711.4
(3)
P 		674
avr 32.6
(-2.9) 		56
(-2.7) 		15.3
(-3) 		765.1
(3) 		750

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 61.1
(-3)
P 		83.2
(-1.6)
P 		156.8
(3)
P 		133.3
(1.5)
P 		115.2
(0.4)
P 		74.9
(-2.1)
P 		96
B 88.8
(-2)
P 		79.7
(-2.7)
P 		86.9
(-2.1)
P 		106.3
(-0.7)
P 		155.7
(3)
P 		75.3
(-3)
P 		80
C 107.9
(0.6)
P 		140.7
(3)
P 		121.2
(1.6)
P 		93
(-0.5)
P 		132.4
(2.4)
P 		57.8
(-3)
P 		83
avr 85.9
(-1.5) 		101.2
(-0.1) 		121.6
(1.8) 		110.9
(0.8) 		134.4
(3) 		69.3
(-3) 		65
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 155
1 2 3 4 range
A 14.2
(-2.4)
A 		8.6
(-2.7)
A 		1.4
(-3)
A 		125.1
(3)
P 		124
B 5
(-2.8)
A 		21.6
(-2.2)
A 		0.7
(-3)
A 		155.3
(3)
P 		155
C 14.3
(-2.9)
A 		11.8
(-3)
A 		13.1
(-2.9)
A 		120
(3)
P 		108
avr 11.2
(-2.7) 		14
(-2.6) 		5.1
(-3) 		133.5
(3) 		128

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 11.3
(-3)
P 		29.6
(1.7)
P 		34.1
(2.8)
P 		33.8
(2.7)
P 		34.8
(3)
P 		18.2
(-1.2)
P 		24
B 30.9
(-1.6)
P 		26.5
(-2.2)
P 		21.1
(-3)
P 		28.2
(-2)
P 		63.3
(3)
P 		28.1
(-2)
P 		42
C 40
(2.3)
P 		42.9
(3)
P 		30.9
(0)
P 		18.7
(-3)
P 		42.8
(3)
P 		19.3
(-2.9)
P 		24
avr 27.4
(-1.7) 		33
(-0.3) 		28.7
(-1.4) 		26.9
(-1.8) 		47
(3) 		21.9
(-3) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189643_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13346
Sequence Type Exemplar sequence
Representative Public ID F08H9.3 NCBI
Target Description F08H9.3 /REP_DB=WormBase Gene ID /WP=CE09267 /TR=Q19227 /GB=CAB01146.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=heat shock protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074184(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074184 NCBI Caenorhabditis elegans F08H9.3 (F08H9.3) mRNA, complete cds. 11/11 None
SNAP00000029728 ENSEMBL cdna:SNAP chromosome:CEL140:V:14464633:14465357:-1 11/11 None
GENEFINDER00000029739 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14464337:14465357:-1 11/11 None
F08H9.3 ENSEMBL cdna:known chromosome:CEL140:V:14464633:14465357:-1 gene:F08H9.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14464632-14465357(-) 100.0 100.0
Public Domain and Genome References
Gene Title heat shock protein
Ensembl F08H9.3
Entrez Gene 184214 Entrez gene
SwissProt Q19227 EMBL-EBI
WormBase CE09267 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01146 Hypothetical protein F08H9.3 [Caenorhabditis elegans] ref|NP_506585.1| F08H9.3 [Caenorhabditis elegans] 3.0E-80
blast CAB01146 Hypothetical protein F08H9.3 [Caenorhabditis elegans] ref|NP_506585.1| F08H9.3 [Caenorhabditis elegans] 4.0E-70
blast CAB01147 Hypothetical protein F08H9.4 [Caenorhabditis elegans] ref|NP_506586.1| F08H9.4 [Caenorhabditis elegans] 2.0E-28
blast CAB01147 Hypothetical protein F08H9.4 [Caenorhabditis elegans] ref|NP_506586.1| F08H9.4 [Caenorhabditis elegans] 6.0E-28
blast CAE60891 Hypothetical protein CBG04606 [Caenorhabditis briggsae] 8.0E-27
Protein Domains
Database ID Description E-Value
Pfam IPR002068 EMBL-EBI Heat shock protein Hsp20 2.7E-22
Sequence
Target Sequence 
>C. ELEGANS:189643_AT
ttcgacccgcgaactcttggtgaaatggtatttggagataatgggagattggatcgagaa
tatgtaccaatttcagagaataatgatgatttgtcagattgtcggaatgagattgtcgat
actcatgagaaattttctgtgaatctcaacgtccccgatgtaaaaccagaagaactgaaa
attaatttagaaggaagaaaattgtcgataaaagctgaacatcaagaaattgaaaacgat
aatatttctacaacccaaacatattcaaaatcaatagtcttgccagaagatgttgacgtc
acacacctttcatcaaatttat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGACCCGCGAACTCTTGGTGAAA 432 693 31 Antisense
CCCGCGAACTCTTGGTGAAATGGTA 425 259 36 Antisense
GATGATTTGTCAGATTGTCGGAATG 533 409 115 Antisense
GAGATTGTCGATACTCATGAGAAAT 198 397 139 Antisense
ATTTTCTGTGAATCTCAACGTCCCC 125 15 162 Antisense
TCAACGTCCCCGATGTAAAACCAGA 168 597 176 Antisense
GATAATATTTCTACAACCCAAACAT 121 421 268 Antisense
TACAACCCAAACATATTCAAAATCA 598 641 279 Antisense
AATCAATAGTCTTGCCAGAAGATGT 560 169 299 Antisense
AAGATGTTGACGTCACACACCTTTC 76 155 317 Antisense
GTCACACACCTTTCATCAAATTTAT 303 465 328 Antisense
Sequence Source Affymetrix Proprietary Database