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[189640_at] phosphate permease

Description W05H5.3 /REP_DB=WormBase Gene ID /WP=CE21261 /TR=P91840 /GB=CAB03482.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=phosphate permease
Cluster 6grp_total > 14      4grp_total > 11      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 143
1 2 3 4 range
A 69.3
(-2.5)
A 		57.8
(-3)
A 		79.5
(-2)
A 		191.1
(3)
A 		133
B 87.4
(3)
A 		67.9
(-0)
A 		48.6
(-3)
A 		55.3
(-2)
A 		39
C 55
(-3)
A 		88.9
(0)
A 		54.9
(-3)
A 		122.6
(3)
M 		68
avr 70.6
(-2.1) 		71.5
(-2) 		61
(-3) 		123
(3) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 88.1
(3)
A 		69.8
(1.6)
A 		32.8
(-1.3)
A 		29.3
(-1.6)
A 		17.3
(-2.5)
A 		11.5
(-3)
A 		77
B 49
(2.3)
A 		52.4
(2.8)
A 		12.4
(-2.6)
A 		54.1
(3)
A 		10.2
(-2.9)
A 		9.3
(-3)
A 		45
C 23.2
(2)
A 		15.8
(-3)
A 		18.6
(-1.1)
A 		24.7
(3)
A 		22.3
(1.4)
A 		22.3
(1.4)
A 		9
avr 53.4
(3) 		46
(1.9) 		21.3
(-1.9) 		36
(0.3) 		16.6
(-2.7) 		14.4
(-3) 		39
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 25.2
(1.9)
A 		12.6
(-3)
A 		20.8
(0.2)
A 		28
(3)
A 		15
B 27.9
(3)
A 		16.8
(-1)
A 		11.3
(-3)
A 		11.8
(-2.8)
A 		17
C 18.2
(-3)
A 		25.6
(3)
A 		19.1
(-2.3)
A 		20.7
(-1)
M 		7
avr 23.8
(3) 		18.3
(-1.9) 		17.1
(-3) 		20.2
(-0.2) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 16.2
(0.7)
A 		24.8
(3)
A 		7.1
(-1.8)
A 		7.4
(-1.7)
A 		5.2
(-2.3)
A 		2.8
(-3)
A 		22
B 17.1
(2.9)
A 		17.4
(3)
A 		3
(-3)
A 		14.3
(1.7)
A 		4.1
(-2.5)
A 		3.5
(-2.8)
A 		14
C 8.6
(3)
A 		4.8
(-2.8)
A 		4.7
(-3)
A 		5
(-2.5)
A 		7.2
(0.8)
A 		7.5
(1.3)
A 		4
avr 14
(2.1) 		15.7
(3) 		4.9
(-2.8) 		8.9
(-0.7) 		5.5
(-2.5) 		4.6
(-3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189640_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3316
Sequence Type Exemplar sequence
Representative Public ID W05H5.3 NCBI
Target Description W05H5.3 /REP_DB=WormBase Gene ID /WP=CE21261 /TR=P91840 /GB=CAB03482.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=phosphate permease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064245(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064245 NCBI Caenorhabditis elegans W05H5.3 (W05H5.3) mRNA, complete cds. 11/11 None
SNAP00000019745 ENSEMBL cdna:SNAP chromosome:CEL140:II:12445235:12447672:1 11/11 None
GENEFINDER00000019751 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:12445235:12451310:1 11/11 None
W05H5.3 ENSEMBL cdna:known chromosome:CEL140:II:12445235:12447672:1 gene:W05H5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12445231-12447669(+) 100.0 100.0
Public Domain and Genome References
Gene Title phosphate permease
Ensembl W05H5.3
Entrez Gene 174883 Entrez gene
SwissProt P91840 EMBL-EBI
WormBase CE21261 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6817 phosphate transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5315 inorganic phosphate transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03482 Hypothetical protein W05H5.3 [Caenorhabditis elegans] ref|NP_496646.1| W05H5.3 [Caenorhabditis elegans] 0.0
blast CAE73510 Hypothetical protein CBG20968 [Caenorhabditis briggsae] 0.0
blast CAE73510 Hypothetical protein CBG20968 [Caenorhabditis briggsae] 1.0E-167
blast CAH60791 Hypothetical protein W05H5.8 [Caenorhabditis elegans] ref|NP_001022395.1| Serpentine Receptor, class E (epsilon) family member (sre-34) [Caenorhabditis elegans] 1.0E-167
blast AAA92320 Hypothetical protein B0222.3 [Caenorhabditis elegans] ref|NP_505371.2| B0222.3 [Caenorhabditis elegans] 1.0E-100
Protein Domains
Database ID Description E-Value
Pfam IPR001204 EMBL-EBI Phosphate transporter 1.0E-126
Pfam IPR001204 EMBL-EBI Phosphate transporter 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.5E-57
Pfam IPR001204 EMBL-EBI Phosphate transporter 1.0E-126
Pfam IPR004151 EMBL-EBI C. elegans Sre G protein-coupled chemoreceptor 1.9E-114
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496646.1 10 39-58,65-87,118-140,145-167,177-199,211-230,245-267,356-374,406-428,493-515
W05H5.3 10 39-58,65-87,118-140,145-167,177-199,211-230,245-267,356-374,406-428,493-515
SNAP00000019745 8 39-58,65-87,118-140,174-196,211-230,281-299,331-353,418-440
GENEFINDER00000019751 21 39-58,65-87,118-140,145-167,177-199,211-230,245-267,321-339,371-393,503-525,535-557,577-599,643-665,686-708,718-740,789-811,864-886,899-921,931-950,985-1007,1022-1041
Sequence
Target Sequence 
>C. ELEGANS:189640_AT
cctctcctacacatttccaattgttccacgtggatggttgagacgggcggtctactgggc
gttcccgccaatgcgaaatgacgatcaaaaagccgttagactgttcagcttcctgcaaat
tttgaccgcctgctttgctggatttgctcacggagccaatgatatttcaaattgtgttgg
accggttagagatttggtgcatatgtataatgaaggttatcgggaggataataccaagtt
gaatataactgtatatatcttattacttaccactttggcggttgtcatgggaatttggac
ccttggagtccgggtcattcggactgtggggaagttttcgaagatgaacccagctacagg
cttctccgttcaattcggagctgccattactgcgctagtcaacaatatgtataacatccc
agagagcggcacccattgtctagtcggctcaatctttgggcttggattagttcggtccgg
gccgatattggaatggcatactgttaaatatgtattcgtatcttggatattgactattcc
cggttcaggtctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCTCTCCTACACATTTCCAATTGTT 334 267 978 Antisense
GGCGTTCCCGCCAATGCGAAATGAC 466 541 1035 Antisense
GTTAGACTGTTCAGCTTCCTGCAAA 26 447 1072 Antisense
TTCCTGCAAATTTTGACCGCCTGCT 509 695 1087 Antisense
TACTTACCACTTTGGCGGTTGTCAT 203 643 1241 Antisense
GGACCCTTGGAGTCCGGGTCATTCG 404 511 1274 Antisense
AGATGAACCCAGCTACAGGCTTCTC 571 67 1319 Antisense
CTGCCATTACTGCGCTAGTCAACAA 593 235 1358 Antisense
CTAGTCGGCTCAATCTTTGGGCTTG 11 225 1417 Antisense
GGATTAGTTCGGTCCGGGCCGATAT 547 505 1441 Antisense
ATTGACTATTCCCGGTTCAGGTCTC 403 3 1506 Antisense
Sequence Source Affymetrix Proprietary Database