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[189639_at] DNAJ protein like

Description B0035.14 /REP_DB=WormBase Gene ID /WP=CE05169 /TR=Q17438 /GB=CAA97416.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=DNAJ protein like
Cluster 6grp_highest_10_10 > 81      6grp_high1_10_10 > 18      4grp_highest_8_8 > 52      4grp_high_8_8 > 17      6grp_highest_nonorm > 82      6grp_total > 28      4grp_total > 31      6grp_highest_sub > Cu      6grp_cgc > 02      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 185
1 2 3 4 range
A 378.6
(2.7)
P 		319.4
(-0.1)
P 		258.4
(-3)
P 		385.2
(3)
P 		127
B 359.5
(3)
P 		239.3
(-2.4)
P 		227.1
(-3)
P 		265.5
(-1.3)
P 		132
C 412.2
(3)
P 		321.9
(-3)
P 		358.8
(-0.5)
P 		353.2
(-0.9)
P 		90
avr 383.4
(3) 		293.5
(-2.3) 		281.4
(-3) 		334.6
(0.1) 		102

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 254
1 2 3 4 5 6 range
A 403.7
(3)
P 		390.2
(2.2)
P 		304
(-3)
P 		305.6
(-2.9)
P 		361.7
(0.5)
P 		363
(0.6)
P 		100
B 448.6
(3)
P 		335.3
(-0.4)
P 		245.8
(-3)
P 		328.3
(-0.6)
P 		381.5
(1)
P 		333
(-0.4)
P 		203
C 500.2
(3)
P 		363.1
(-2)
P 		371.6
(-1.7)
P 		402.8
(-0.6)
P 		337.8
(-2.9)
P 		336.1
(-3)
P 		164
avr 450.8
(3) 		362.9
(-0.7) 		307.1
(-3) 		345.6
(-1.4) 		360.3
(-0.8) 		344
(-1.5) 		144
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 85
1 2 3 4 range
A 137.9
(3)
P 		69.3
(-2.1)
P 		67.6
(-2.2)
P 		56.4
(-3)
P 		82
B 114.6
(3)
P 		59.1
(-2.4)
P 		52.6
(-3)
P 		56.6
(-2.6)
P 		62
C 136.5
(3)
P 		92.6
(-0.4)
P 		125
(2.1)
P 		59.6
(-3)
P 		77
avr 129.7
(3) 		73.7
(-1.7) 		81.7
(-1) 		57.5
(-3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 74.4
(-2.3)
P 		138.8
(3)
P 		66.2
(-3)
P 		77.5
(-2.1)
P 		109.1
(0.5)
P 		88.4
(-1.2)
P 		73
B 156.1
(3)
P 		111.6
(0.2)
P 		59.7
(-3)
P 		87
(-1.3)
P 		155
(2.9)
P 		124.2
(1)
P 		96
C 185.4
(3)
P 		110.8
(-1.3)
P 		94.6
(-2.2)
P 		81
(-3)
P 		109.3
(-1.4)
P 		112.5
(-1.2)
P 		104
avr 138.6
(3) 		120.4
(1.3) 		73.5
(-3) 		81.8
(-2.2) 		124.5
(1.7) 		108.4
(0.2) 		65
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189639_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8455
Sequence Type Exemplar sequence
Representative Public ID B0035.14 NCBI
Target Description B0035.14 /REP_DB=WormBase Gene ID /WP=CE05169 /TR=Q17438 /GB=CAA97416.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=DNAJ protein like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069721(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069721 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-1) (dnj-1) mRNA, complete cds. 11/11 None
SNAP00000035828 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11299014:11304304:1 11/11 None
GENEFINDER00000035852 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11299014:11304304:1 11/11 None
B0035.14 ENSEMBL cdna:known chromosome:CEL140:IV:11299007:11304758:1 gene:B0035.14 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11299019-11304310(+) 100.0 100.0
Public Domain and Genome References
Gene Title DNAJ protein like
Gene Symbol dnj-1
Ensembl B0035.14
Entrez Gene 178040 Entrez gene
SwissProt Q17438 EMBL-EBI
WormBase CE05169 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251622_AT DNAJ heat shock N-terminal domain-containing protein at
CANINE_2:CFA.17078.1.S1_S_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 12 cfa
CANINE_2:CFA.17870.1.S1_S_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 12 cfa
CANINE_2:CFAAFFX.22311.1.S1_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 12 cfa
CANINE_2:CFAAFFX.22335.1.S1_S_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 12 cfa
CANINE_2:CFAAFFX.22335.1.S1_X_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 12 cfa
DROSOPHILA_2:1636708_AT dm
DROSGENOME1:149868_AT dm
CHICKEN:GGAAFFX.26199.1.S1_AT DnaJ (Hsp40) homolog, subfamily B, member 12 gga
CHICKEN:GGAAFFX.26199.2.S1_S_AT DnaJ (Hsp40) homolog, subfamily B, member 12 gga
HG-U133A_2:202867_S_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HG-U133A:202867_S_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HG-U133_PLUS_2:202867_S_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HU35KSUBA:RC_AA489063_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HU35KSUBD:RC_W73276_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
U133_X3P:G8923029_3P_A_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
U133_X3P:HS.7960.1.A1_3P_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
U133_X3P:HS.7960.0.A3_3P_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HG-U133A_2:202865_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HG-U133_PLUS_2:202865_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HG-U133A:202865_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HG-U133A_2:202866_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HG-U133_PLUS_2:202866_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HG-U133A:202866_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HG-U133A_2:214338_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HG-U133_PLUS_2:214338_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HG-U133A:214338_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HG-U95AV2:35466_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HG-U95AV2:35467_G_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HG-U95AV2:35468_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
HG-U95B:46288_AT DnaJ (Hsp40) homolog, subfamily B, member 12 hs
MOE430A:1421032_A_AT DnaJ (Hsp40) homolog, subfamily B, member 12 mm
MOUSE430A_2:1421032_A_AT DnaJ (Hsp40) homolog, subfamily B, member 12 mm
MOUSE430_2:1421032_A_AT DnaJ (Hsp40) homolog, subfamily B, member 12 mm
MU11KSUBB:MSA.31754.0_S_AT DnaJ (Hsp40) homolog, subfamily B, member 12 mm
MG-U74BV2:116090_AT DnaJ (Hsp40) homolog, subfamily B, member 12 mm
MG-U74CV2:135718_AT DnaJ (Hsp40) homolog, subfamily B, member 12 mm
MOE430A:1435886_AT DnaJ (Hsp40) homolog, subfamily B, member 12 mm
MOUSE430A_2:1435886_AT DnaJ (Hsp40) homolog, subfamily B, member 12 mm
MOUSE430_2:1435886_AT DnaJ (Hsp40) homolog, subfamily B, member 12 mm
MU19KSUBC:TC39425_AT DnaJ (Hsp40) homolog, subfamily B, member 12, mRNA (cDNA clone MGC:35897 IMAGE:5043868) mm
RAT230_2:1374727_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 12 rn
RAE230A:1374727_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 12 rn
RG-U34B:RC_AI070335_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 12 rn
RG-U34C:RC_AA818949_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 12 rn
RAE230A:1389487_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 12 rn
RAT230_2:1389487_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 12 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6457 protein folding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA97416 Hypothetical protein B0035.14 [Caenorhabditis elegans] ref|NP_502122.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-1) [Caenorhabditis elegans] 0.0
blast CAE62048 Hypothetical protein CBG06064 [Caenorhabditis briggsae] 0.0
blast NP_001015991 DnaJ (Hsp40) homolog, subfamily B, member 12 [Xenopus tropicalis] 2.0E-66
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 1.00000001954148E-24
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.6E-32
Sequence
Target Sequence 
>C. ELEGANS:189639_AT
tgaagcagcgaattcacacactccgacaacaagaagacgtggtggaggacatggtgcatt
ctttgaacatgattatgcacatggattcgaggccgaattcacaccagaagagattttcaa
catgttcttcggaggcggattccctaccgaacaagttcgccgacgtgctcgatatgctca
acagcaacagtatcatcattatgaacaacagcaaagtccatatggaccacttcttcaatt
attgccacttatcgccattatggtcattggattgttagcccagttgatggttggagaacc
tgcgtattcacttcatcaaacttcaaaattcacaataaaacgactgacagcagatttacg
agttccatattttgtacgaacggactttgagacctcgtatcgaggacgaatccgacaagt
tgagcaacaagtggaagatgattatattcagaatttacggatgaattgttataaagaaca
aaatctaaaagaaacaaaactttatcgagctcgttggatgcgtgacgaagcaatgatgcg
tgatgcagaacgaacacctcttcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAAGCAGCGAATTCACACACTCCG 90 575 618 Antisense
GGAGGACATGGTGCATTCTTTGAAC 627 515 661 Antisense
TTCGAGGCCGAATTCACACCAGAAG 111 695 703 Antisense
GATTCCCTACCGAACAAGTTCGCCG 141 429 755 Antisense
CGACGTGCTCGATATGCTCAACAGC 646 251 778 Antisense
AGTCCATATGGACCACTTCTTCAAT 238 61 832 Antisense
AATTATTGCCACTTATCGCCATTAT 541 175 854 Antisense
TGGAGAACCTGCGTATTCACTTCAT 557 549 909 Antisense
GAACGGACTTTGAGACCTCGTATCG 226 347 995 Antisense
TTATCGAGCTCGTTGGATGCGTGAC 164 679 1119 Antisense
TGATGCAGAACGAACACCTCTTCCA 246 567 1158 Antisense
Sequence Source Affymetrix Proprietary Database