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[189636_s_at] myosin

Description F29D10.4 /REP_DB=WormBase Gene ID /WP=CE05763 /GEN=hum-1 /TR=Q19901 /GB=CAB00095.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=myosin
Cluster 6grp_highest_10_10 > 85      6grp_high1_10_10 > 57      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 84      6grp_total > 57      4grp_total > 54      6grp_highest_sub > Cu      6grp_cgc > 27      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 457
1 2 3 4 range
A 687.6
(3)
P 		664.2
(2.5)
P 		428.7
(-3)
P 		459.9
(-2.3)
P 		259
B 654.5
(3)
P 		455.4
(-0.3)
P 		426
(-0.8)
P 		295.9
(-3)
P 		359
C 752.7
(3)
P 		455.8
(-1.5)
P 		508.5
(-0.7)
P 		360.5
(-3)
P 		392
avr 698.3
(3) 		525.1
(-0.2) 		454.4
(-1.5) 		372.1
(-3) 		326

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 558
1 2 3 4 5 6 range
A 741.8
(0.1)
P 		680.7
(-0.6)
P 		471.1
(-3)
P 		511.3
(-2.5)
P 		526.6
(-2.4)
P 		987.5
(3)
P 		516
B 670.7
(1.1)
P 		556.3
(-0.8)
P 		474.8
(-2.2)
P 		429.4
(-3)
P 		468.9
(-2.3)
P 		779.9
(3)
P 		351
C 708.7
(0.1)
P 		631.2
(-1.1)
P 		677.4
(-0.4)
P 		520
(-3)
P 		517.1
(-3)
P 		884.7
(3)
P 		368
avr 707.1
(0.3) 		622.7
(-0.9) 		541.1
(-2.2) 		486.9
(-3) 		504.2
(-2.7) 		884
(3) 		397
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 190
1 2 3 4 range
A 250.4
(3)
P 		144.2
(-0.5)
P 		112.1
(-1.5)
P 		67.3
(-3)
P 		183
B 208.7
(3)
P 		112.5
(-1)
P 		98.7
(-1.5)
P 		63
(-3)
P 		146
C 249.2
(3)
P 		131
(-0.8)
P 		177.2
(0.7)
P 		60.8
(-3)
P 		188
avr 236.1
(3) 		129.2
(-0.7) 		129.3
(-0.7) 		63.7
(-3) 		172

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 194
1 2 3 4 5 6 range
A 136.7
(-1.5)
P 		242.1
(3)
P 		102.6
(-3)
P 		129.7
(-1.8)
P 		158.9
(-0.6)
P 		240.4
(2.9)
P 		140
B 233.4
(1.1)
P 		185.2
(-0.6)
P 		115.3
(-2.9)
P 		113.7
(-3)
P 		190.5
(-0.4)
P 		290.8
(3)
P 		177
C 262.7
(2)
P 		192.6
(-0.2)
P 		172.4
(-0.9)
P 		104.6
(-3)
P 		167.3
(-1)
P 		296.2
(3)
P 		192
avr 210.9
(0.6) 		206.6
(0.4) 		130.1
(-2.5) 		116
(-3) 		172.2
(-0.9) 		275.8
(3) 		160
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189636_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1251
Sequence Type Exemplar sequence
Representative Public ID F29D10.4 NCBI
Target Description F29D10.4 /REP_DB=WormBase Gene ID /WP=CE05763 /GEN=hum-1 /TR=Q19901 /GB=CAB00095.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=myosin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059992(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059992 NCBI Caenorhabditis elegans F29D10.4 (myosin) mRNA, complete cds. 11/11 None
SNAP00000040558 ENSEMBL cdna:SNAP chromosome:CEL140:I:8854146:8859219:-1 11/11 None
GENEFINDER00000040560 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8854146:8859219:-1 11/11 None
F29D10.4 ENSEMBL cdna:known chromosome:CEL140:I:8853931:8859219:-1 gene:F29D10.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8854076-8859150(-) 100.0 100.0
Public Domain and Genome References
Gene Title myosin
Gene Symbol hum-1
Ensembl F29D10.4
Entrez Gene 172698 Entrez gene
SwissProt Q19901 EMBL-EBI
WormBase CE05763 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1605741_AT similar to myosin IE cfa
CANINE_2:CFA.19780.1.S1_S_AT similar to myosin IE cfa
CANINE_2:CFAAFFX.25468.1.S1_AT similar to myosin IE cfa
CANINE_2:CFAAFFX.25468.1.S1_S_AT similar to myosin IE cfa
HUGENEFL:U14391_AT myosin IE hs
HG-U133A:203072_AT myosin IE hs
HG-U133_PLUS_2:203072_AT myosin IE hs
HG-FOCUS:203072_AT myosin IE hs
HG-U133A_2:203072_AT myosin IE hs
U133_X3P:G4826843_3P_AT myosin IE hs
HG-U95AV2:37631_AT myosin IE hs
HG-U95D:74646_AT myosin IE hs
HG-U133_PLUS_2:232471_AT Myosin IE hs
HG-U133B:232471_AT Myosin IE hs
HG-U133_PLUS_2:215408_AT Myosin IE hs
HG-U133A_2:215408_AT Myosin IE hs
HG-U133A:215408_AT Myosin IE hs
U133_X3P:HS2.373427.1.S1_3P_AT Myosin IE hs
U133_X3P:HS.306759.0.S1_3P_AT Myosin IE hs
U133_X3P:HS.287468.0.S1_3P_AT Myosin IE hs
HG-U133_PLUS_2:1556623_AT Myosin IE hs
HG-U133_PLUS_2:215405_AT Myosin IE hs
HG-U133A_2:215405_AT Myosin IE hs
HG-U133A:215405_AT Myosin IE hs
HG-U133_PLUS_2:237136_AT Myosin IE hs
HG-U133B:237136_AT Myosin IE hs
HG-U133_PLUS_2:241493_AT Myosin IE hs
HG-U133B:241493_AT Myosin IE hs
HG-U95E:72249_AT Myosin IE hs
HG-U95D:73942_AT Myosin IE hs
HG-U95D:77032_AT Myosin IE hs
U133_X3P:HS.224142.0.A1_3P_AT Myosin IE hs
U133_X3P:HS.252813.0.A1_3P_AT Myosin IE hs
RG-U34A:RC_AA955302_AT myosin IE rn
RG-U34A:RC_AI175935_AT myosin IE rn
RG-U34A:RC_AI059137_AT myosin IE rn
RAE230A:1370933_AT myosin IE rn
RAT230_2:1370933_AT myosin IE rn
YEAST_2:1774876_AT One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16459 myosin inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3774 motor activity inferred from electronic annotation QuickGO AmiGO
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB00095 Hypothetical protein F29D10.4 [Caenorhabditis elegans] ref|NP_492393.1| F29D10.4 [Caenorhabditis elegans] 0.0
blast CAE66984 Hypothetical protein CBG12380 [Caenorhabditis briggsae] 0.0
blast AAA97925 hum-1 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001452 EMBL-EBI SH3 3.2E-17
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 1.0E-126
Pfam IPR010926 EMBL-EBI Myosin tail 2 2.4E-72
Sequence
Target Sequence 
>C. ELEGANS:189636_S_AT
gaaacgatggaacttcgtctgcagagaagacattgaaaccaaatggaaaagttttgaatg
ttagcattggtactggattaccgaatactacaaggccatcaactgaacgtccacaaggtg
ggtacaccccgcgaagagatcaattgagaacatcaacacgacgaacgaaacagaataatc
aaagttatggacaaaatggccaatctcaggcaatgagagctccggttcccgctcatggaa
tgaataacaattacaatcagacaccagctccagtatcgactaatcatcaatatagccaag
aacctgcgcgaattccagtgatgggaaacgtcataaatcagttgaataacatgaatctat
ctggaaacggaaactctccagctggacgaggacctccgccagcgcgtggaccaaaacctc
ctccaccagcaaagccaaagcttaatcctgtcgttattgctgtctatccatatgaagctc
aagatgttgatgagctcagctttgaagctggagctgaaattgaactcatgaacaaagatg
cttctggatggtggcaaggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAACGATGGAACTTCGTCTGCAGA 417 357 2705 Antisense
GTACACCCCGCGAAGAGATCAATTG 163 461 2826 Antisense
TCCGGTTCCCGCTCATGGAATGAAT 95 595 2925 Antisense
CAATTACAATCAGACACCAGCTCCA 381 179 2952 Antisense
ACCAGCTCCAGTATCGACTAATCAT 161 87 2967 Antisense
GAACCTGCGCGAATTCCAGTGATGG 56 347 3004 Antisense
GAAACGGAAACTCTCCAGCTGGACG 48 355 3068 Antisense
CCTCCACCAGCAAAGCCAAAGCTTA 284 267 3124 Antisense
GCCAAAGCTTAATCCTGTCGTTATT 440 277 3138 Antisense
GTCGTTATTGCTGTCTATCCATATG 620 473 3154 Antisense
GATGCTTCTGGATGGTGGCAAGGAA 235 413 3241 Antisense
Sequence Source Affymetrix Proprietary Database