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[189633_at] cytochrome P-450

Description B0213.16 /REP_DB=WormBase Gene ID /WP=CE16786 /TR=O61204 /GB=AAC47940.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P-450
Cluster 6grp_total > 95      4grp_total > 11      6grp_cgc > 19      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 241
1 2 3 4 range
A 68.2
(-1.6)
P 		40.6
(-3)
A 		46.3
(-2.7)
A 		155.6
(3)
P 		115
B 87.7
(-0.4)
P 		114.2
(0.9)
A 		32.3
(-3)
A 		158.8
(3)
P 		127
C 72.2
(-2.2)
P 		47.4
(-2.8)
A 		39.8
(-3)
A 		273.7
(3)
P 		234
avr 76
(-1.6) 		67.4
(-1.9) 		39.5
(-3) 		196
(3) 		157

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 99.5
(3)
A 		77.4
(-1.9)
A 		72.4
(-3)
A 		74.5
(-2.5)
P 		84.8
(-0.3)
P 		95.5
(2.1)
P 		27
B 62.9
(-1.8)
P 		78.2
(-0.1)
A 		52.6
(-3)
A 		105.2
(3)
M 		88.4
(1.1)
P 		70.5
(-1)
P 		53
C 55
(-3)
A 		100
(3)
P 		59.3
(-2.4)
A 		61.7
(-2.1)
A 		62.3
(-2)
A 		92.2
(2)
P 		45
avr 72.5
(-0.3) 		85.2
(2.8) 		61.4
(-3) 		80.5
(1.6) 		78.5
(1.2) 		86.1
(3) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 24.8
(3)
P 		8.8
(-3)
A 		12.1
(-1.8)
A 		22.8
(2.3)
P 		16
B 28
(1.7)
P 		28.2
(1.7)
A 		7.5
(-3)
A 		33.9
(3)
P 		26
C 23.9
(-1.1)
P 		13.6
(-3)
A 		13.9
(-2.9)
A 		46.2
(3)
P 		33
avr 25.6
(0.7) 		16.9
(-1.5) 		11.2
(-3) 		34.3
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 18.3
(-1.7)
A 		27.5
(3)
A 		15.8
(-3)
A 		18.9
(-1.4)
P 		25.6
(2)
P 		23.3
(0.8)
P 		12
B 21.9
(-0.6)
P 		26
(0.4)
A 		12.8
(-3)
A 		27.9
(0.9)
M 		35.9
(3)
P 		26.3
(0.5)
P 		23
C 20.4
(-0.4)
A 		30.5
(2.9)
P 		15.1
(-2.1)
A 		12.4
(-3)
A 		20.1
(-0.5)
A 		30.9
(3)
P 		19
avr 20.2
(-0.5) 		28
(3) 		14.6
(-3) 		19.7
(-0.7) 		27.2
(2.6) 		26.8
(2.5) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189633_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14926
Sequence Type Exemplar sequence
Representative Public ID B0213.16 NCBI
Target Description B0213.16 /REP_DB=WormBase Gene ID /WP=CE16786 /TR=O61204 /GB=AAC47940.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P-450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071703(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071703 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-34A10) (cyp-34A10) mRNA, complete cds. 11/11 None
SNAP00000025855 ENSEMBL cdna:SNAP chromosome:CEL140:V:3970585:3972803:-1 11/11 None
GENEFINDER00000025871 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3970585:3972803:-1 11/11 None
B0213.16 ENSEMBL cdna:known chromosome:CEL140:V:3970585:3972803:-1 gene:B0213.16 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3970584-3972803(-) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P-450
Gene Symbol cyp-34A10
Ensembl B0213.16
Entrez Gene 181852 Entrez gene
SwissProt O61204 EMBL-EBI
WormBase CE16786 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:267505_AT cytochrome P450 family protein at
CANINE:1585921_AT similar to cytochrome P450, family 2, subfamily A, polypeptide 13 cfa
CANINE_2:CFA.14938.1.A1_AT similar to cytochrome P450, family 2, subfamily A, polypeptide 13 cfa
CANINE_2:CFAAFFX.8510.1.S1_S_AT similar to cytochrome P450, family 2, subfamily A, polypeptide 13 cfa
CANINE_2:CFAAFFX.8540.1.S1_AT similar to cytochrome P450, family 2, subfamily A, polypeptide 13 cfa
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC47940 Cytochrome p450 family protein 34A10 [Caenorhabditis elegans] ref|NP_504104.1| CYtochrome P450 family member (cyp-34A10) [Caenorhabditis elegans] 0.0
blast CAE58180 Hypothetical protein CBG01272 [Caenorhabditis briggsae] 0.0
blast AAC47939 Cytochrome p450 family protein 34A9 [Caenorhabditis elegans] ref|NP_504103.2| CYtochrome P450 family member (cyp-34A9) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-152
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-156
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.5E-94
Pfam IPR001128 EMBL-EBI Cytochrome P450 3.8E-86
Sequence
Target Sequence 
>C. ELEGANS:189633_AT
gtattcgatctatggcaggctggccaagaaacaacatcaacgacactgacgtgggcattc
tgttgcctactgaaccatccaaatgtggtgaagaagttgagagctgaactgatgaagttg
actggaggcaatagacatgttggattgaatgatagagcagataccccatacttaaatgca
gtttgtaatgaagtgcaacgcattgcttcaattctcaacatcaatcttttccgaaaaatc
gaacaggatacagaaatcgctggccagccacttgcttctggttgcgttgtcacaactcaa
atgtcaatgcttcatacggatgtagaagtttacaaaaatccaactgaattcagaccggaa
agattcctcgaaaataacactttggagaagaagttgattccgttcggaattggcaaacga
tcctgtcccggagagtcattggctagagctgagctgtatttgatcctgtcaaatgtgatc
ctggacgttgaaatcgaggcagtcggatcgattccaaaaattttaacgagcaatccattt
gggctactgagaagaccgccgagttac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTATTCGATCTATGGCAGGCTGGCC 240 453 916 Antisense
GACACTGACGTGGGCATTCTGTTGC 538 367 957 Antisense
TGTTGCCTACTGAACCATCCAAATG 707 559 976 Antisense
GATAGAGCAGATACCCCATACTTAA 123 425 1066 Antisense
AGTGCAACGCATTGCTTCAATTCTC 97 61 1107 Antisense
CAATTCTCAACATCAATCTTTTCCG 571 179 1124 Antisense
TACAGAAATCGCTGGCCAGCCACTT 76 643 1164 Antisense
GCTTCTGGTTGCGTTGTCACAACTC 148 307 1189 Antisense
GGAATTGGCAAACGATCCTGTCCCG 444 533 1321 Antisense
TCCTGTCCCGGAGAGTCATTGGCTA 347 593 1336 Antisense
GCTACTGAGAAGACCGCCGAGTTAC 44 301 1458 Antisense
Sequence Source Affymetrix Proprietary Database