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[189626_s_at] glutamine synthase

Description C28D4.3 /REP_DB=WormBase Gene ID /WP=CE08432 /TR=O02225 /GB=CAB05127.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=glutamine synthase
Cluster 6grp_total > 98      4grp_total > 01      6grp_cgc > 69      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 284
1 2 3 4 range
A 6.6
(-3)
A 		7.1
(-3)
A 		6.2
(-3)
A 		245.8
(3)
P 		240
B 21.9
(-2.6)
A 		3.4
(-3)
A 		52.4
(-2)
A 		287.1
(3)
P 		284
C 3.2
(-3)
A 		17.5
(-2.6)
A 		38
(-2.1)
A 		237.9
(3)
P 		235
avr 10.6
(-3) 		9.3
(-3) 		32.2
(-2.4) 		256.9
(3) 		248

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2236
1 2 3 4 5 6 range
A 15.6
(-3)
A 		26.2
(-3)
A 		79
(-2.8)
P 		191.1
(-2.5)
P 		1393.5
(1.3)
P 		1942
(3)
P 		1926
B 29.4
(-2.9)
A 		12.9
(-3)
A 		70.5
(-2.8)
P 		189.7
(-2.5)
P 		1282.2
(0.9)
P 		1953.5
(3)
P 		1941
C 23.9
(-3)
A 		5.3
(-3)
A 		131.4
(-2.7)
P 		181.9
(-2.5)
P 		1309.7
(0.5)
P 		2241.4
(3)
P 		2236
avr 23
(-3) 		14.8
(-3) 		93.6
(-2.8) 		187.6
(-2.5) 		1328.5
(0.9) 		2045.6
(3) 		2031
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 2.4
(-2.9)
A 		1.6
(-3)
A 		1.6
(-3)
A 		36
(3)
P 		34
B 7
(-2.4)
A 		0.8
(-3)
A 		12.1
(-1.9)
A 		61.2
(3)
P 		60
C 1.1
(-3)
A 		5
(-2.4)
A 		13.2
(-1.1)
A 		40.1
(3)
P 		39
avr 3.5
(-2.9) 		2.5
(-3) 		9
(-2.1) 		45.8
(3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 749
1 2 3 4 5 6 range
A 2.9
(-3)
A 		9.3
(-2.9)
A 		17.2
(-2.8)
P 		48.5
(-2.4)
P 		420.5
(2.3)
P 		472.8
(3)
P 		470
B 10.2
(-3)
A 		4.3
(-3)
A 		17.1
(-2.9)
P 		50.2
(-2.6)
P 		520.9
(1.3)
P 		728.4
(3)
P 		724
C 8.9
(-2.9)
A 		1.6
(-3)
A 		33.4
(-2.7)
P 		36.6
(-2.7)
P 		423.8
(0.4)
P 		750.3
(3)
P 		749
avr 7.3
(-3) 		5.1
(-3) 		22.6
(-2.8) 		45.1
(-2.6) 		455.1
(1.2) 		650.5
(3) 		645
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189626_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9962
Sequence Type Exemplar sequence
Representative Public ID C28D4.3 NCBI
Target Description C28D4.3 /REP_DB=WormBase Gene ID /WP=CE08432 /TR=O02225 /GB=CAB05127.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=glutamine synthase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069332(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069332 NCBI Caenorhabditis elegans GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-6) (gln-6) mRNA, complete cds. 11/11 A 
SNAP00000030228 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9739539:9740829:-1 11/11 None
GENEFINDER00000030237 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9739539:9744958:-1 11/11 None
C28D4.3.1 ENSEMBL cdna:known chromosome:CEL140:IV:9739301:9740872:-1 gene:C28D4.3 11/11 None
C28D4.3.2 ENSEMBL cdna:known chromosome:CEL140:IV:9739423:9740870:-1 gene:C28D4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9739544-9740835(-) 100.0 100.0
Public Domain and Genome References
Gene Title glutamine synthase
Gene Symbol gln-6
Ensembl C28D4.3
Entrez Gene 177808 Entrez gene
SwissProt O02225 EMBL-EBI
WormBase CE08432 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:16503_AT glutamine synthetase (GS2) at
ATH1-121501:249710_AT glutamine synthetase (GS2) at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9399 nitrogen fixation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4356 glutamate-ammonia ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05127 Hypothetical protein C28D4.3 [Caenorhabditis elegans] ref|NP_501733.1| GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-6) [Caenorhabditis elegans] 0.0
blast CAB02317 Hypothetical protein F26D10.10 [Caenorhabditis elegans] ref|NP_503065.1| GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-5) [Caenorhabditis elegans] 0.0
blast CAE72665 Hypothetical protein CBG19879 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008147 EMBL-EBI Glutamine synthetase, beta-Grasp domain 7.9E-32
Pfam IPR008146 EMBL-EBI Glutamine synthetase, catalytic domain 1.2E-93
Pfam IPR008147 EMBL-EBI Glutamine synthetase, beta-Grasp domain 7.9E-32
Pfam IPR008146 EMBL-EBI Glutamine synthetase, catalytic domain 1.2E-93
Pfam IPR009658 EMBL-EBI Protein of unknown function DUF1248 7.5E-114
Pfam IPR009658 EMBL-EBI Protein of unknown function DUF1248 1.5E-100
Sequence
Target Sequence 
>C. ELEGANS:189626_S_AT
actgcggtattggggccgatcgtgcatttggtcgtgaagtcgttgagactcattatcgtg
cttgtctccacgcaggaatcaccatattcggatcgaacgcggaggtcactccaggacaat
gggagttccaaattggtacttgcctgggtattgaaatgggcgatcagctgtggatggcta
gatacatattgcacagagttgccgaacagttcggagtttgcgtctctcttgaccccaaac
cacgcgttacaatgggcgattggaacggcgctggctgtcatacaaacttctcaacgattg
atatgcgtcgaccagatgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTGCGGTATTGGGGCCGATCGTGC 647 95 509 Antisense
GGCCGATCGTGCATTTGGTCGTGAA 282 543 522 Antisense
AGACTCATTATCGTGCTTGTCTCCA 644 71 554 Antisense
TTGTCTCCACGCAGGAATCACCATA 336 709 570 Antisense
TTCGGATCGAACGCGGAGGTCACTC 458 643 595 Antisense
AGTTCCAAATTGGTACTTGCCTGGG 230 63 632 Antisense
ATGGGCGATCAGCTGTGGATGGCTA 130 51 664 Antisense
AACAGTTCGGAGTTTGCGTCTCTCT 635 135 713 Antisense
ATGGGCGATTGGAACGGCGCTGGCT 111 51 760 Antisense
GGCTGTCATACAAACTTCTCAACGA 334 541 781 Antisense
GATTGATATGCGTCGACCAGATGGA 323 435 804 Antisense
Sequence Source Affymetrix Proprietary Database