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[189623_at] diphthine synthase

Description B0491.7 /REP_DB=WormBase Gene ID /WP=CE02109 /TR=Q17514 /GB=CAA90089.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=diphthine synthase
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 75      4grp_highest_8_8 > 34      4grp_high_8_8 > 49      6grp_highest_nonorm > 72      6grp_total > 75      4grp_total > 62      6grp_highest_sub > Da      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 221
1 2 3 4 range
A 586.5
(3)
P 		462.3
(-3)
P 		468
(-2.7)
P 		516.9
(-0.4)
P 		124
B 618.2
(3)
P 		504.7
(-0.1)
P 		600.8
(2.5)
P 		397.6
(-3)
P 		221
C 580
(3)
P 		551.6
(1.7)
P 		546.3
(1.4)
P 		451.4
(-3)
P 		129
avr 594.9
(3) 		506.2
(-0.8) 		538.4
(0.6) 		455.3
(-3) 		140

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 188
1 2 3 4 5 6 range
A 328.4
(-3)
P 		362.6
(-1.6)
P 		371
(-1.3)
P 		479.5
(3)
P 		330.2
(-2.9)
P 		430
(1)
P 		151
B 372
(-0.1)
P 		413.1
(1.4)
P 		291.6
(-3)
P 		410.8
(1.3)
P 		380.4
(0.2)
P 		458
(3)
P 		166
C 384.7
(-0.6)
P 		386
(-0.5)
P 		371
(-1.2)
P 		431
(1.4)
P 		328.3
(-3)
P 		469.5
(3)
P 		141
avr 361.7
(-2) 		387.2
(-0.6) 		344.5
(-3) 		440.4
(2.3) 		346.3
(-2.9) 		452.5
(3) 		108
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 213.6
(3)
P 		100.4
(-1.9)
P 		122.4
(-1)
P 		75.7
(-3)
P 		138
B 197.1
(3)
P 		124.7
(-0.9)
P 		139.2
(-0.1)
P 		84.7
(-3)
P 		112
C 192
(3)
P 		158.6
(1.3)
P 		190.4
(2.9)
P 		76.1
(-3)
P 		116
avr 200.9
(3) 		127.9
(-0.6) 		150.7
(0.5) 		78.8
(-3) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 110
1 2 3 4 5 6 range
A 60.5
(-3)
P 		129
(3)
P 		80.8
(-1.2)
P 		121.6
(2.4)
P 		99.6
(0.4)
P 		104.7
(0.9)
P 		69
B 129.4
(0.5)
P 		137.5
(1)
P 		70.8
(-3)
P 		108.8
(-0.7)
P 		154.6
(2)
P 		170.8
(3)
P 		100
C 142.6
(1.8)
P 		117.8
(-0.4)
P 		94.4
(-2.3)
P 		86.7
(-3)
P 		106.2
(-1.3)
P 		157.2
(3)
P 		71
avr 110.8
(-0.2) 		128.1
(1.4) 		82
(-3) 		105.7
(-0.7) 		120.1
(0.7) 		144.2
(3) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189623_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2675
Sequence Type Exemplar sequence
Representative Public ID B0491.7 NCBI
Target Description B0491.7 /REP_DB=WormBase Gene ID /WP=CE02109 /TR=Q17514 /GB=CAA90089.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=diphthine synthase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064026(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064026 NCBI Caenorhabditis elegans B0491.7 (B0491.7) mRNA, complete cds. 11/11 None
SNAP00000040223 ENSEMBL cdna:SNAP chromosome:CEL140:II:11350098:11351130:1 11/11 None
GENEFINDER00000040224 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11350098:11351130:1 11/11 None
B0491.7 ENSEMBL cdna:known chromosome:CEL140:II:11350098:11351130:1 gene:B0491.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11350094-11351127(+) 100.0 100.0
Public Domain and Genome References
Gene Title diphthine synthase
Ensembl B0491.7
Entrez Gene 174737 Entrez gene
SwissProt Q17514 EMBL-EBI
WormBase CE02109 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:14007_AT diphthine synthase, putative (DPH5) at
ATH1-121501:253490_AT diphthine synthase, putative (DPH5) at
CANINE_2:CFA.19507.1.S1_AT similar to Probable diphthine synthase (Diphthamide biosynthesis methyltransferase) cfa
CANINE_2:CFAAFFX.30605.1.S1_AT similar to Probable diphthine synthase (Diphthamide biosynthesis methyltransferase) cfa
CANINE_2:CFAAFFX.30605.1.S1_S_AT similar to Probable diphthine synthase (Diphthamide biosynthesis methyltransferase) cfa
DROSOPHILA_2:1627661_AT Diphthamide methyltransferase dm
DROSGENOME1:153752_AT Diphthamide methyltransferase dm
CHICKEN:GGAAFFX.3273.3.S1_S_AT similar to Probable diphthine synthase (Diphthamide biosynthesis methyltransferase) (CGI-30) (AD-018) (HSPC143) (NPD015) gga
HG-U133_PLUS_2:224196_X_AT DPH5 homolog (S. cerevisiae) hs
HG-U133B:224196_X_AT DPH5 homolog (S. cerevisiae) hs
HG-U133B:223671_X_AT DPH5 homolog (S. cerevisiae) hs
HG-U133_PLUS_2:223671_X_AT DPH5 homolog (S. cerevisiae) hs
HG-U133_PLUS_2:224060_S_AT DPH5 homolog (S. cerevisiae) hs
HG-U133B:224060_S_AT DPH5 homolog (S. cerevisiae) hs
HU35KSUBA:W07461_AT DPH5 homolog (S. cerevisiae) hs
HG-U133A:219590_X_AT DPH5 homolog (S. cerevisiae) hs
HG-U133_PLUS_2:219590_X_AT DPH5 homolog (S. cerevisiae) hs
HG-U133A_2:219590_X_AT DPH5 homolog (S. cerevisiae) hs
U133_X3P:G7688688_3P_X_AT DPH5 homolog (S. cerevisiae) hs
U133_X3P:G7688688_3P_A_AT DPH5 homolog (S. cerevisiae) hs
U133_X3P:HS.262642.0.A1_3P_AT DPH5 homolog (S. cerevisiae) hs
HG-U133A_2:222360_AT DPH5 homolog (S. cerevisiae) hs
HG-U133_PLUS_2:222360_AT DPH5 homolog (S. cerevisiae) hs
HG-U133A:222360_AT DPH5 homolog (S. cerevisiae) hs
HG-U95C:65148_AT DPH5 homolog (S. cerevisiae) hs
HU35KSUBC:RC_AA069386_AT DPH5 homolog (S. cerevisiae) hs
HU35KSUBC:RC_H69862_AT DPH5 homolog (S. cerevisiae) hs
MOUSE430A_2:1439049_AT DPH5 homolog (S. cerevisiae) mm
MOE430A:1439049_AT DPH5 homolog (S. cerevisiae) mm
MOUSE430_2:1439049_AT DPH5 homolog (S. cerevisiae) mm
MOE430A:1452839_AT DPH5 homolog (S. cerevisiae) mm
MOUSE430A_2:1452839_AT DPH5 homolog (S. cerevisiae) mm
MOUSE430_2:1452839_AT DPH5 homolog (S. cerevisiae) mm
MU11KSUBA:C80186_RC_AT DPH5 homolog (S. cerevisiae) mm
MU11KSUBA:AA183138_F_AT DPH5 homolog (S. cerevisiae) mm
MG-U74AV2:100912_AT DPH5 homolog (S. cerevisiae) mm
MG-U74BV2:116727_AT DPH5 homolog (S. cerevisiae) mm
MG-U74BV2:106850_AT DPH5 homolog (S. cerevisiae) mm
MG-U74CV2:136401_AT DPH5 homolog (S. cerevisiae) (Dph5), mRNA mm
MOE430B:1438823_AT DPH5 homolog (S. cerevisiae) (Dph5), mRNA mm
MOUSE430_2:1438823_AT DPH5 homolog (S. cerevisiae) (Dph5), mRNA mm
MOE430B:1459549_AT DPH5 homolog (S. cerevisiae) (Dph5), mRNA mm
MOUSE430_2:1459549_AT DPH5 homolog (S. cerevisiae) (Dph5), mRNA mm
MU19KSUBB:TC32041_AT DPH5 homolog (S. cerevisiae) (Dph5), mRNA mm
RG-U34B:RC_AA996583_AT similar to RIKEN cDNA 2410012M04 (predicted) rn
RAE230A:1390272_AT similar to RIKEN cDNA 2410012M04 (predicted) rn
RAT230_2:1390272_AT similar to RIKEN cDNA 2410012M04 (predicted) rn
YEAST_2:1771781_AT Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
17183 peptidyl-diphthamide biosynthesis from peptidyl-histidine inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4164 diphthine synthase activity inferred from electronic annotation QuickGO AmiGO
8168 methyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA90089 Hypothetical protein B0491.7 [Caenorhabditis elegans] ref|NP_496427.1| B0491.7 [Caenorhabditis elegans] 1.0E-157
blast CAE59523 Hypothetical protein CBG02918 [Caenorhabditis briggsae] 1.0E-150
blast XP_783189 PREDICTED: similar to Probable diphthine synthase (Diphthamide biosynthesis methyltransferase) [Strongylocentrotus purpuratus] 4.0E-98
Protein Domains
Database ID Description E-Value
Pfam IPR000878 EMBL-EBI Uroporphyrin-III C/tetrapyrrole (Corrin/Porphyrin) methyltransferase 4.0E-25
Sequence
Target Sequence 
>C. ELEGANS:189623_AT
gacgtagctctacttgtcgttggagacccgttcggagcaactacacatgcggatctggtg
ttgcgtgctaagcaacagaatattccggtcaaagtgattcacaatgcttccatcatgaat
gcagttggctgttgtggactacaactctacaatttcggagaaacagtttcaattgtaatg
tggactgacgagtggcagccggagagctattacgataaaatcgcgttgaatcgtaaacgt
ggaatgcacactttatgcctgctggatatcaaaaccaaagagcaaactgtcgaaaatatg
atgcgtgggcggaagatcttcgagccagccaggtatcaaaagtgctccgaagcggctcga
caactgctgaccatttatgagagacgaaaagccaagggagaagagtgtgcctatgatgag
aacacgatggttgtcggcttggcaagagttggatgggacaatcagaagatcgtatacgcg
agtatgaaagatatgagtgagatggagatgggcgaacctctacattcactgattattcct
ggtgaaacccatcctttgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGTAGCTCTACTTGTCGTTGGAG 121 377 247 Antisense
GGAGACCCGTTCGGAGCAACTACAC 463 519 268 Antisense
CACATGCGGATCTGGTGTTGCGTGC 93 197 290 Antisense
GTGGACTACAACTCTACAATTTCGG 127 487 380 Antisense
GAATGCACACTTTATGCCTGCTGGA 248 331 488 Antisense
ATCTTCGAGCCAGCCAGGTATCAAA 219 31 562 Antisense
CAAAAGTGCTCCGAAGCGGCTCGAC 481 189 583 Antisense
GCTCGACAACTGCTGACCATTTATG 271 297 601 Antisense
GAACACGATGGTTGTCGGCTTGGCA 268 351 666 Antisense
TGGAGATGGGCGAACCTCTACATTC 209 549 749 Antisense
TTCCTGGTGAAACCCATCCTTTGGA 521 697 782 Antisense
Sequence Source Affymetrix Proprietary Database