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[189621_at]

Description R05F9.3_rc /REP_DB=WormBase Gene ID /WP=CE04805 /GEN=msp-32 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /REVCOMP /DEF=major sperm protein
Cluster 6grp_high1_10_10 > 67      6grp_total > 68      4grp_total > 03      6grp_cgc > 38      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 220
1 2 3 4 range
A 76.1
(-2.3)
P 		65.7
(-2.7)
A 		59.8
(-3)
A 		196.2
(3)
P 		136
B 95
(0.2)
P 		59.9
(-3)
A 		81.8
(-1)
A 		125.6
(3)
P 		66
C 74.2
(-2.6)
P 		86.7
(-2.3)
A 		60.7
(-3)
A 		279.9
(3)
P 		219
avr 81.8
(-2.4) 		70.8
(-2.8) 		67.4
(-3) 		200.6
(3) 		133

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 83.3
(-1.3)
P 		63
(-2.5)
A 		63.9
(-2.5)
P 		68.3
(-2.2)
P 		55
(-3)
A 		157.1
(3)
P 		102
B 82.1
(-0.8)
A 		61.9
(-1.9)
P 		43.4
(-3)
A 		54.4
(-2.4)
M 		56.6
(-2.2)
P 		148.6
(3)
P 		105
C 55.3
(-2.9)
A 		81.6
(-1.2)
P 		54
(-3)
A 		61.1
(-2.5)
P 		63.2
(-2.4)
M 		144.8
(3)
P 		91
avr 73.6
(-1.8) 		68.8
(-2.1) 		53.8
(-3) 		61.3
(-2.5) 		58.3
(-2.7) 		150.2
(3) 		96
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 27.7
(2.6)
P 		14.3
(-3)
A 		15.6
(-2.5)
A 		28.7
(3)
P 		14
B 30.3
(3)
P 		14.8
(-3)
A 		19
(-1.4)
A 		26.8
(1.6)
P 		16
C 24.6
(-2.2)
P 		24.9
(-2.1)
A 		21.2
(-3)
A 		47.2
(3)
P 		26
avr 27.5
(0.5) 		18
(-3) 		18.6
(-2.8) 		34.2
(3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 15.4
(-2.6)
P 		22.4
(-0.9)
A 		13.9
(-3)
P 		17.3
(-2.2)
P 		16.6
(-2.3)
A 		38.3
(3)
P 		24
B 28.6
(-0.6)
A 		20.6
(-1.7)
P 		10.5
(-3)
A 		14.4
(-2.5)
M 		23
(-1.3)
P 		55.4
(3)
P 		45
C 20.5
(-1.6)
A 		24.9
(-0.9)
P 		13.8
(-2.8)
A 		12.3
(-3)
P 		20.4
(-1.7)
M 		48.5
(3)
P 		36
avr 21.5
(-1.5) 		22.6
(-1.3) 		12.7
(-3) 		14.7
(-2.7) 		20
(-1.7) 		47.4
(3) 		35
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189621_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28121
Sequence Type Exemplar sequence
Representative Public ID R05F9.3 NCBI
Target Description R05F9.3_rc /REP_DB=WormBase Gene ID /WP=CE04805 /GEN=msp-32 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /REVCOMP /DEF=major sperm protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Design Representative Id based pipeline to a Affymetrix Proprietary Database identifier using 1 transcript(s).
Annotation Grade This is a grade R annotation.
Annotation Transcript Cluster (# of Matching Probes) R05F9.3
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
R05F9.3 R05F9.3_rc /REP_DB=WormBase Gene ID /WP=CE04805 /GEN=msp-32 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /REVCOMP /DEF=major sperm protein A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_062490 11/11 Negative Strand Matching Probes A 
SNAP00000043869 11/11 Negative Strand Matching Probes None
GENEFINDER00000043883 11/11 Negative Strand Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4898599-4899172(-) 65.1 100.0
Public Domain and Genome References
Ensembl R05F9.3
Sequence
Target Sequence 
>C. ELEGANS:189621_AT
aggttcttgcgacggaccataccgtctccttggaaccattcccgacggaattgcttagca
gcaccatccggggtgttggtccactcaacagtgatacgatcgttgttagtgtcctcctgt
ccaaaagcgaaggcatcgcaggaaactgccagaagaacagcttcctttggatcgagcact
ccacatggtggatcaactccaagtctcttcatattggtggtcttgataccgtatccaata
cggcgagccgaggagttaatcaccttgatgtggtaggtgtgcttgtcgtcgtatggtgca
ttgaagacgatcttcgtacccggctgggtctggatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGTTCTTGCGACGGACCATACCGT 640 55 35 Antisense
TCCTTGGAACCATTCCCGACGGAAT 166 581 61 Antisense
GGAATTGCTTAGCAGCACCATCCGG 320 533 81 Antisense
GATACGATCGTTGTTAGTGTCCTCC 132 425 127 Antisense
TGTCCTCCTGTCCAAAAGCGAAGGC 526 555 144 Antisense
GAACAGCTTCCTTTGGATCGAGCAC 226 351 189 Antisense
GGATCGAGCACTCCACATGGTGGAT 330 511 203 Antisense
GATCAACTCCAAGTCTCTTCATATT 48 423 225 Antisense
ATTGGTGGTCTTGATACCGTATCCA 556 3 247 Antisense
GTGTGCTTGTCGTCGTATGGTGCAT 261 487 311 Antisense
TTCGTACCCGGCTGGGTCTGGATCA 625 693 347 Antisense
Sequence Source Affymetrix Proprietary Database