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[189620_s_at] --- /// cysteine protease

Description Y53H1B.6 /REP_DB=WormBase Gene ID /WP=CE24433 /TR=Q9U247 /GB=CAB60415.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=cysteine protease
Cluster 6grp_total > 43      4grp_total > 12      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 2.5
(-3)
A 		18.3
(3)
A 		3.1
(-2.8)
A 		8.2
(-0.8)
A 		16
B 27.9
(3)
A 		2.7
(-3)
A 		4.1
(-2.7)
A 		3
(-2.9)
A 		25
C 2.4
(-3)
A 		5.1
(-2.7)
A 		9.7
(-2.1)
A 		51
(3)
A 		49
avr 10.9
(-0.9) 		8.7
(-1.8) 		5.6
(-3) 		20.7
(3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 4.3
(-2.7)
A 		2.4
(-3)
A 		36.5
(3)
A 		5.9
(-2.4)
A 		7.4
(-2.1)
A 		2.5
(-3)
A 		34
B 2.7
(-1.5)
A 		1.2
(-3)
A 		7.4
(3)
A 		2.3
(-1.9)
A 		2.9
(-1.4)
A 		1.8
(-2.4)
A 		6
C 21.2
(3)
A 		6.2
(-1.6)
A 		6.7
(-1.5)
A 		7.3
(-1.3)
A 		2
(-2.9)
A 		1.7
(-3)
A 		20
avr 9.4
(-0) 		3.3
(-2.5) 		16.9
(3) 		5.2
(-1.7) 		4.1
(-2.2) 		2
(-3) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 0.9
(-2.8)
A 		4
(3)
A 		0.8
(-3)
A 		1.2
(-2.3)
A 		3
B 8.9
(3)
A 		0.7
(-2.9)
A 		1
(-2.7)
A 		0.6
(-3)
A 		8
C 0.8
(-3)
A 		1.5
(-2.5)
A 		3.4
(-1)
A 		8.6
(3)
A 		8
avr 3.5
(3) 		2.1
(-1.7) 		1.7
(-3) 		3.5
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 0.8
(-2.8)
A 		0.9
(-2.8)
A 		8
(3)
A 		1.5
(-2.3)
A 		2.2
(-1.7)
A 		0.6
(-3)
A 		7
B 0.9
(-0.9)
A 		0.4
(-3)
A 		1.8
(3)
A 		0.6
(-2.1)
A 		1.2
(0.4)
A 		0.7
(-1.7)
A 		1
C 7.9
(3)
A 		1.9
(-1.9)
A 		1.7
(-2.1)
A 		1.5
(-2.3)
A 		0.7
(-2.9)
A 		0.6
(-3)
A 		7
avr 3.2
(1.9) 		1.1
(-2.1) 		3.8
(3) 		1.2
(-1.9) 		1.4
(-1.5) 		0.6
(-3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189620_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.609
Sequence Type Exemplar sequence
Representative Public ID Y53H1B.6 NCBI
Target Description Y53H1B.6 /REP_DB=WormBase Gene ID /WP=CE24433 /TR=Q9U247 /GB=CAB60415.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=cysteine protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060502(11), NM_060503(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060502 NCBI Caenorhabditis elegans Y53H1B.6 (Y53H1B.6) mRNA, complete cds. 11/11 None
NM_060503 NCBI Caenorhabditis elegans H25P06.4 (H25P06.4) mRNA, complete cds. 11/11 A 
GENEFINDER00000026542 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:11313976:11318996:-1 11/11 None
GENEFINDER00000041760 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:11310591:11313312:1 11/11 None
Y53H1B.6 ENSEMBL cdna:known chromosome:CEL140:I:11311863:11313342:1 gene:Y53H1B.6 11/11 None
H25P06.4 ENSEMBL cdna:known chromosome:CEL140:I:11313944:11317724:-1 gene:H25P06.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11311793-11313273(+) 100.0 100.0
chrI:11316175-11317655(-) 100.0 100.0
Public Domain and Genome References
Gene Title
cysteine protease
Ensembl Y53H1B.6
H25P06.4
Entrez Gene 186784 Entrez gene
190249 Entrez gene
SwissProt Q9XU14 EMBL-EBI
Q9U247 EMBL-EBI
WormBase CE18825 Wormbase
CE24433 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4198 calpain activity inferred from electronic annotation QuickGO AmiGO
46983 protein dimerization activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07236 Hypothetical protein H25P06.4 [Caenorhabditis elegans] ref|NP_492904.1| H25P06.4 [Caenorhabditis elegans] 0.0
blast CAB60415 Hypothetical protein Y53H1B.6 [Caenorhabditis elegans] ref|NP_492903.1| Y53H1B.6 [Caenorhabditis elegans] 1.0E-146
blast CAB07236 Hypothetical protein H25P06.4 [Caenorhabditis elegans] ref|NP_492904.1| H25P06.4 [Caenorhabditis elegans] 1.0E-142
blast CAB60415 Hypothetical protein Y53H1B.6 [Caenorhabditis elegans] ref|NP_492903.1| Y53H1B.6 [Caenorhabditis elegans] 1.0E-141
Protein Domains
Database ID Description E-Value
Pfam IPR008906 EMBL-EBI HAT dimerisation 1.2E-25
Pfam IPR008906 EMBL-EBI HAT dimerisation 1.2E-25
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000026542 1 27-49
GENEFINDER00000041760 1 27-49
Sequence
Target Sequence 
>C. ELEGANS:189620_S_AT
ggacatgattgttgaagcccacttggctggatttttactgtgtacatccaaactatctct
agatgaagagagtgccgaatacatatttaactcgactggcataaagcaaaatcatgcgta
cgcgattctaaattcagttgtgtttgaaaaccacagactcgttcaaattggcaacactca
tgcgtgcgacaaatggaaagagttacataaggaatggaattccctacattatttttataa
taaattttcttccaaactaccgagacgcccatatatcagtcatttggatgatatgagttt
ttggatggatattgatcaatactgcgagcattttagtagtcttactgtatgtgaatatcg
gaaggattggaaagaaattcgcttcagacagactaatgatttgaaacaaagtcgagaaac
tgaggctttgcgaatgactgttgatagaagatgtgagttagtcgttgaggtgactgatcg
agtacttgcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACATGATTGTTGAAGCCCACTTG 523 525 216 Antisense
AGCCCACTTGGCTGGATTTTTACTG 641 75 231 Antisense
CTGTGTACATCCAAACTATCTCTAG 589 239 253 Antisense
AATACATATTTAACTCGACTGGCAT 560 171 293 Antisense
TCATGCGTACGCGATTCTAAATTCA 122 247 327 Antisense
GAAAACCACAGACTCGTTCAAATTG 305 355 361 Antisense
TTGGCAACACTCATGCGTGCGACAA 417 709 383 Antisense
CAAACTACCGAGACGCCCATATATC 405 189 468 Antisense
GAAATTCGCTTCAGACAGACTAATG 200 363 589 Antisense
GAGGCTTTGCGAATGACTGTTGATA 129 405 637 Antisense
GAGGTGACTGATCGAGTACTTGCAA 586 401 682 Antisense
Sequence Source Affymetrix Proprietary Database