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[189619_at] cytochrome P450

Description T10B9.5 /REP_DB=WormBase Gene ID /WP=CE01658 /TR=SW:Q27517 /GB=CAA88606.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 4grp_high_8_8 > 07      6grp_total > 57      4grp_total > 02      6grp_cgc > 37      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 223
1 2 3 4 range
A 37.7
(-3)
A 		39.8
(-2.9)
A 		54.4
(-2.1)
P 		154
(3)
P 		116
B 36.8
(-2.8)
A 		58.7
(-2.2)
A 		28.2
(-3)
A 		251.6
(3)
P 		223
C 35.7
(-2.9)
P 		31.1
(-3)
P 		42.7
(-2.6)
M 		226.9
(3)
P 		196
avr 36.7
(-3) 		43.2
(-2.8) 		41.8
(-2.8) 		210.8
(3) 		174

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 38
1 2 3 4 5 6 range
A 12.6
(-3)
A 		27.8
(0.5)
A 		31.2
(1.3)
M 		38.3
(3)
A 		20.2
(-1.2)
A 		30.4
(1.2)
A 		26
B 35
(3)
A 		16.5
(-0.3)
A 		22.7
(0.8)
A 		1.6
(-3)
A 		24.1
(1)
M 		30.7
(2.2)
A 		33
C 34.1
(1.7)
P 		23.4
(-0.9)
A 		22.2
(-1.2)
A 		39.2
(3)
A 		15
(-3)
P 		39.2
(3)
P 		24
avr 27.2
(0.3) 		22.6
(-1.8) 		25.4
(-0.5) 		26.4
(-0.1) 		19.8
(-3) 		33.4
(3) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 13.7
(-0.8)
A 		8.6
(-3)
A 		14.2
(-0.6)
P 		22.5
(3)
P 		14
B 11.7
(-2.3)
A 		14.5
(-2)
A 		6.5
(-3)
A 		53.6
(3)
P 		47
C 11.8
(-2.4)
P 		8.9
(-3)
P 		14.9
(-1.8)
M 		38.3
(3)
P 		29
avr 12.4
(-2.6) 		10.7
(-3) 		11.9
(-2.7) 		38.1
(3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 2.3
(-3)
A 		9.9
(3)
A 		6.8
(0.6)
M 		9.7
(2.8)
A 		6.1
(-0)
A 		7.4
(1)
A 		8
B 12.2
(3)
A 		5.5
(-0.4)
A 		5.5
(-0.4)
A 		0.4
(-3)
A 		9.8
(1.8)
M 		11.5
(2.6)
A 		12
C 12.6
(2.6)
P 		7.1
(-1.3)
A 		5.6
(-2.4)
A 		7.9
(-0.8)
A 		4.8
(-3)
P 		13.1
(3)
P 		8
avr 9
(0.8) 		7.5
(-1.1) 		6
(-3) 		6
(-3) 		6.9
(-1.9) 		10.7
(3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189619_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3844
Sequence Type Exemplar sequence
Representative Public ID T10B9.5 NCBI
Target Description T10B9.5 /REP_DB=WormBase Gene ID /WP=CE01658 /TR=SW:Q27517 /GB=CAA88606.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063709(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063709 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-13A3) (cyp-13A3) mRNA, complete cds. 11/11 None
GENEFINDER00000024373 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9788809:9795452:-1 11/11 None
T10B9.5 ENSEMBL cdna:known chromosome:CEL140:II:9788809:9793195:-1 gene:T10B9.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000024364 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9788805-9793192(-) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-13A3
Ensembl T10B9.5
Entrez Gene 188359 Entrez gene
SwissProt Q27517 EMBL-EBI
WormBase CE01658 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88606 Hypothetical protein T10B9.5 [Caenorhabditis elegans] ref|NP_496110.1| CYtochrome P450 family member (cyp-13A3) [Caenorhabditis elegans] sp|Q27517|C13A3_CAEEL Putative cytochrome P450 CYP13A3 0.0
blast CAA88604 Hypothetical protein T10B9.2 [Caenorhabditis elegans] ref|NP_496112.1| CYtochrome P450 family member (cyp-13A5) [Caenorhabditis elegans] sp|Q27514|C13A5_CAEEL Putative cytochrome P450 CYP13A5 0.0
blast CAA88603 Hypothetical protein T10B9.1 [Caenorhabditis elegans] ref|NP_496111.1| CYtochrome P450 family member (cyp-13A4) [Caenorhabditis elegans] sp|Q27513|C13A4_CAEEL Putative cytochrome P450 CYP13A4 0.0
Protein Families
Method ID Description E-Value
p450 CP14_CHICK Animalia; CYP1A4; chi; CP14_CHICK; Gallus gallus; P79760 1.0E-132
p450 CP14_CHICK Animalia; CYP1A4; chi; CP14_CHICK; Gallus gallus; P79760 1.0E-133
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 2.6E-24
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.8E-68
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.4E-8
Pfam IPR001128 EMBL-EBI Cytochrome P450 3.2E-12
Pfam IPR001128 EMBL-EBI Cytochrome P450 2.6E-24
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.8E-68
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496110.1 2 5-27,212-234
T10B9.5 2 5-27,212-234
GENEFINDER00000024373 4 5-22,201-223,315-337,523-545
Sequence
Target Sequence 
>C. ELEGANS:189619_AT
gatcattggccaactgtttgttttcttgttagctggatatgatacaactgccctttccct
ctcatattcctcatatctcctggcaagacatccagagattcagaagaaattacaagaaga
agtggatagagaatgtccagaccctgaagtcacttttgatcagctgtcgaaattaaagta
catggaatgtgtgatcaaagaaactttgagattgtacccattagcttcaatagttcacaa
tcggaaatgcatgaaatcaactacagtactcggaatgaaaatcgaagaaggaacaaatgt
tcaagcagatacatggactctacactatgatccaaaattttggggtgaaaatgcaaatga
gttcaagcctgaaagatgggaatctggagacgaacaagctgttgcaaagggagcttacct
tcctttcggacttggaccccgaatttgcattggaatgcgtttggcctatatggaagaaaa
gatgcttctggcacaaattctgaagaaatattctctggaaacaacttttgaaacccatat
tccattgaaactggttggaatcgccaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCATTGGCCAACTGTTTGTTTTC 255 419 957 Antisense
GAAACTTTGAGATTGTACCCATTAG 313 357 1156 Antisense
GTACCCATTAGCTTCAATAGTTCAC 288 459 1170 Antisense
AGCAGATACATGGACTCTACACTAT 516 77 1260 Antisense
GACTCTACACTATGATCCAAAATTT 640 373 1272 Antisense
GCAAAGGGAGCTTACCTTCCTTTCG 528 317 1360 Antisense
CTTGGACCCCGAATTTGCATTGGAA 98 219 1387 Antisense
ATGCGTTTGGCCTATATGGAAGAAA 364 41 1411 Antisense
AGATGCTTCTGGCACAAATTCTGAA 612 67 1436 Antisense
ACAACTTTTGAAACCCATATTCCAT 301 113 1477 Antisense
ATTGAAACTGGTTGGAATCGCCACA 540 3 1500 Antisense
Sequence Source Affymetrix Proprietary Database