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[189615_at]

Description ZK546.2 /REP_DB=WormBase Gene ID /WP=CE07632 /TR=Q23526 /GB=AAA68734.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Adenylate cyclase
Cluster 6grp_highest_10_10 > 57      6grp_high1_10_10 > 74      4grp_highest_8_8 > 62      4grp_high_8_8 > 27      6grp_highest_nonorm > 65      6grp_total > 65      4grp_total > 29      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 268
1 2 3 4 range
A 1107.1
(3)
P 		931
(-3)
P 		983.3
(-1.2)
P 		1104.6
(2.9)
P 		176
B 1078.3
(-3)
P 		1198.9
(3)
P 		1122.2
(-0.8)
P 		1155.5
(0.8)
P 		121
C 1154.4
(3)
P 		1125.5
(2)
P 		982.5
(-3)
P 		1025.4
(-1.5)
P 		172
avr 1113.3
(3) 		1085.1
(1) 		1029.3
(-3) 		1095.2
(1.7) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 550
1 2 3 4 5 6 range
A 1106.1
(-2.7)
P 		1089.3
(-3)
P 		1411
(3)
P 		1213.8
(-0.7)
P 		1191.1
(-1.1)
P 		1244.3
(-0.1)
P 		322
B 1093.4
(-2.2)
P 		1065.6
(-3)
P 		1256.6
(2.6)
P 		1271.2
(3)
P 		1123.3
(-1.3)
P 		1199.2
(0.9)
P 		206
C 953.8
(-3)
P 		1097.5
(-1.4)
P 		1123.5
(-1.1)
P 		1085.3
(-1.6)
P 		1200.2
(-0.3)
P 		1503.7
(3)
P 		550
avr 1051.1
(-3) 		1084.1
(-2.3) 		1263.7
(1.8) 		1190.1
(0.2) 		1171.5
(-0.3) 		1315.7
(3) 		265
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 241
1 2 3 4 range
A 403.1
(3)
P 		202.1
(-2)
P 		257.1
(-0.6)
P 		161.7
(-3)
P 		241
B 343.9
(3)
P 		296.1
(0.1)
P 		260
(-2.2)
P 		246.2
(-3)
P 		98
C 382.1
(3)
P 		323.6
(1.3)
P 		342.4
(1.9)
P 		172.9
(-3)
P 		209
avr 376.4
(3) 		273.9
(-0.4) 		286.5
(0) 		193.6
(-3) 		183

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 300
1 2 3 4 5 6 range
A 203.9
(-3)
P 		387.4
(3)
P 		307.3
(0.4)
P 		307.8
(0.4)
P 		359.4
(2.1)
P 		302.9
(0.2)
P 		184
B 380.5
(-0)
P 		354.7
(-1)
P 		305.1
(-3)
P 		336.7
(-1.7)
P 		456.4
(3)
P 		447.2
(2.6)
P 		151
C 353.5
(-0.2)
P 		334.9
(-0.5)
P 		286
(-1.6)
P 		218.2
(-3)
P 		388.4
(0.6)
P 		503.4
(3)
P 		285
avr 312.6
(-1.8) 		359
(0.3) 		299.5
(-2.5) 		287.6
(-3) 		401.4
(2.2) 		417.8
(3) 		130
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189615_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5774
Sequence Type Exemplar sequence
Representative Public ID ZK546.2 NCBI
Target Description ZK546.2 /REP_DB=WormBase Gene ID /WP=CE07632 /TR=Q23526 /GB=AAA68734.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Adenylate cyclase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027354(11), NM_170985(11), NM_062508(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027354 NCBI Caenorhabditis elegans ZK546.2c (ZK546.2) mRNA, complete cds. 11/11 None
NM_170985 NCBI Caenorhabditis elegans ZK546.2a (ZK546.2) mRNA, complete cds. 11/11 A 
NM_062508 NCBI Caenorhabditis elegans ZK546.2b (ZK546.2) mRNA, complete cds. 11/11 None
SNAP00000034951 ENSEMBL cdna:SNAP chromosome:CEL140:II:4946012:4947936:1 11/11 None
GENEFINDER00000034964 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4946012:4947936:1 11/11 None
ZK546.2c ENSEMBL cdna:known chromosome:CEL140:II:4946012:4948055:1 gene:ZK546.2 11/11 A 
ZK546.2a ENSEMBL cdna:known chromosome:CEL140:II:4946012:4947936:1 gene:ZK546.2 11/11 A 
ZK546.2b ENSEMBL cdna:known chromosome:CEL140:II:4947072:4947936:1 gene:ZK546.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4947202-4947934(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZK546.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAR12987 Hypothetical protein ZK546.2c [Caenorhabditis elegans] ref|NP_001022525.1| ZK546.2c [Caenorhabditis elegans] 0.0
blast AAR12986 Hypothetical protein ZK546.2a [Caenorhabditis elegans] ref|NP_740983.2| ZK546.2a [Caenorhabditis elegans] 0.0
blast T27904 hypothetical protein ZK546.2b - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.019
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0018
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0016
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.076
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.99
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0033
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0015
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 1.5E-4
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.66
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.99
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0033
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0015
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 1.5E-4
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.66
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.99
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0033
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0015
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 1.5E-4
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.66
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0016
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.076
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.99
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0033
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0015
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 1.5E-4
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.66
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.99
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0033
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.0015
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 1.5E-4
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.66
Sequence
Target Sequence 
>C. ELEGANS:189615_AT
aaccattagctgatgttctccgcaacttggaactcagtgaaaacaaaattcgagaaattc
ctatctttattggtcaattttctcaactcaagcaacttcatttggctaataactgtcttg
aatttctacctgatgaacttggatcgatgaaaaagctggagattttgaatttggcgggaa
acaagctgaaagcgttgcccgacacaattgtcggttgcaccgatttgaaaaccatcgact
tatcatccaacgttttcaccgtttttcctgtagcagtgattggctgtttgcaattggata
tactcaatttaaatggaaaccaaattgaatctctccctgacgatatatccaatttgaaag
ttatcgagttaagtctgaatcaaaatcgactgagctccttaaatccatcaaatcttgcca
aaactacacgactgaggactcttcgtctcgatgaaaactgtttggagaagtcggagttca
ctcgcgatttactaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACCATTAGCTGATGTTCTCCGCAA 669 147 80 Antisense
TTCTCCGCAACTTGGAACTCAGTGA 671 691 95 Antisense
GGTCAATTTTCTCAACTCAAGCAAC 15 505 151 Antisense
TGAAAGCGTTGCCCGACACAATTGT 54 573 266 Antisense
CATCGACTTATCATCCAACGTTTTC 630 211 312 Antisense
TTTCACCGTTTTTCCTGTAGCAGTG 326 673 333 Antisense
GAATCTCTCCCTGACGATATATCCA 188 329 406 Antisense
ATCGACTGAGCTCCTTAAATCCATC 298 37 464 Antisense
AAATCTTGCCAAAACTACACGACTG 500 117 489 Antisense
ACACGACTGAGGACTCTTCGTCTCG 115 111 505 Antisense
CGGAGTTCACTCGCGATTTACTAGA 33 243 551 Antisense
Sequence Source Affymetrix Proprietary Database