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[189610_at] poly(A) polymerase

Description T15H9.6 /REP_DB=WormBase Gene ID /WP=CE01669 /TR=Q09995 /GB=CAA87419.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=poly(A) polymerase
Cluster 6grp_total > 68      4grp_total > 02      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 18.6
(-2)
A 		6.9
(-3)
A 		11
(-2.7)
A 		79.4
(3)
P 		73
B 20.9
(-1.3)
A 		36.3
(3)
A 		15.2
(-2.9)
A 		15
(-3)
A 		21
C 7.6
(-2.8)
A 		5.2
(-3)
A 		18.4
(-1.8)
A 		70.6
(3)
P 		65
avr 15.7
(-2.9) 		16.1
(-2.8) 		14.9
(-3) 		55
(3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 20
(-0.4)
A 		5.6
(-2.4)
A 		6.4
(-2.3)
A 		1.6
(-3)
A 		11.7
(-1.6)
A 		43.7
(3)
A 		42
B 14.8
(-0.7)
A 		23
(0.7)
A 		1.9
(-2.9)
A 		1
(-3)
A 		15.9
(-0.5)
A 		37.1
(3)
P 		36
C 19.1
(-1.9)
A 		20.1
(-1.7)
A 		30.3
(0.7)
A 		35.5
(1.9)
A 		14.5
(-3)
A 		40.3
(3)
P 		26
avr 18
(-1.9) 		16.2
(-2.2) 		12.9
(-3) 		12.7
(-3) 		14
(-2.7) 		40.4
(3) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 6.8
(0.1)
A 		1.5
(-3)
A 		2.9
(-2.2)
A 		11.6
(3)
P 		10
B 6.7
(0.6)
A 		9
(3)
A 		3.5
(-2.7)
A 		3.2
(-3)
A 		6
C 2.5
(-2.4)
A 		1.5
(-3)
A 		6.4
(-0.2)
A 		11.9
(3)
P 		10
avr 5.3
(-1.4) 		4
(-3) 		4.3
(-2.6) 		8.9
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 3.7
(-1.1)
A 		2
(-2.1)
A 		1.4
(-2.4)
A 		0.4
(-3)
A 		3.5
(-1.2)
A 		10.6
(3)
A 		10
B 5.2
(-0.8)
A 		7.6
(0.2)
A 		0.5
(-2.9)
A 		0.3
(-3)
A 		6.5
(-0.2)
A 		13.8
(3)
P 		14
C 7.1
(-1.4)
A 		6.1
(-2)
A 		7.7
(-1)
A 		7.1
(-1.4)
A 		4.7
(-3)
A 		13.5
(3)
P 		9
avr 5.3
(-1.4) 		5.2
(-1.4) 		3.2
(-2.6) 		2.6
(-3) 		4.9
(-1.6) 		12.6
(3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189610_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2873
Sequence Type Exemplar sequence
Representative Public ID T15H9.6 NCBI
Target Description T15H9.6 /REP_DB=WormBase Gene ID /WP=CE01669 /TR=Q09995 /GB=CAA87419.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=poly(A) polymerase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063666(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063666 NCBI Caenorhabditis elegans T15H9.6 (T15H9.6) mRNA, complete cds. 11/11 None
SNAP00000036795 ENSEMBL cdna:SNAP chromosome:CEL140:II:9607642:9609306:-1 11/11 None
GENEFINDER00000036805 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9607642:9609306:-1 11/11 None
T15H9.6 ENSEMBL cdna:known chromosome:CEL140:II:9607642:9609306:-1 gene:T15H9.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9607638-9609303(-) 100.0 100.0
Public Domain and Genome References
Gene Title poly(A) polymerase
Ensembl T15H9.6
Entrez Gene 174513 Entrez gene
SwissProt Q09995 EMBL-EBI
WormBase CE01669 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:18787_AT nucleotidyltransferase family protein at
ATH1-121501:266659_AT nucleotidyltransferase family protein at
HG-U133A:208271_AT poly(A) polymerase beta (testis specific) hs
HG-U133_PLUS_2:208271_AT poly(A) polymerase beta (testis specific) hs
HG-FOCUS:208271_AT poly(A) polymerase beta (testis specific) hs
HG-U133A_2:208271_AT poly(A) polymerase beta (testis specific) hs
U133_X3P:G9910477_3P_AT poly(A) polymerase beta (testis specific) hs
U133_X3P:HS.307366.0.A1_3P_X_AT poly(A) polymerase beta (testis specific) hs
U133_X3P:HS.307366.0.A1_3P_AT poly(A) polymerase beta (testis specific) hs
HG-U133_PLUS_2:242158_AT poly(A) polymerase beta (testis specific) hs
HG-U133B:242158_AT poly(A) polymerase beta (testis specific) hs
HG-U95E:68583_AT poly(A) polymerase beta (testis specific) hs
HG-U95D:69785_AT poly(A) polymerase beta (testis specific) hs
MOUSE430_2:1449208_AT poly (A) polymerase beta (testis specific) mm
MOE430A:1449208_AT poly (A) polymerase beta (testis specific) mm
MOUSE430A_2:1449208_AT poly (A) polymerase beta (testis specific) mm
MG-U74AV2:161033_AT poly (A) polymerase beta (testis specific) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6350 transcription inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4652 polynucleotide adenylyltransferase activity inferred from electronic annotation QuickGO AmiGO
16779 nucleotidyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA87419 Hypothetical protein T15H9.6 [Caenorhabditis elegans] ref|NP_496067.1| T15H9.6 [Caenorhabditis elegans] 0.0
blast CAA90398 Hypothetical protein F43G6.5 [Caenorhabditis elegans] emb|CAB60582.1| Hypothetical protein F43G6.5 [Caenorhabditis elegans] ref|NP_496524.1| F43G6.5 [Caenorhabditis elegans] 0.0
blast CAA16309 Hypothetical protein Y32F6A.3 [Caenorhabditis elegans] ref|NP_505683.1| Poly-A Polymerase family member (pap-1) [Caenorhabditis elegans] 1.0E-169
Protein Domains
Database ID Description E-Value
Pfam IPR007012 EMBL-EBI Poly(A) polymerase, central region 2.8E-109
Pfam IPR007010 EMBL-EBI Poly(A) polymerase, predicted RNA-binding domain 3.3E-64
Pfam IPR002934 EMBL-EBI DNA polymerase, beta-like region 1.9E-13
Sequence
Target Sequence 
>C. ELEGANS:189610_AT
ggaacattggagtggcgtgatttgcttgaagaaatcaactttttcagtaaatatcatcat
tttattgcactgaaattgaaggctgaatctgtgaaggaggagctggcattcggtggattc
ttcgagtcaagaatccggcaacttgtgcaaattttggagaaaaaccaggtgattcaagtg
gctcaaatcaatccaagaaagttcaaggatgtgaaggatccgaagaagtcgatttggttc
atcggattggagtttgttgcaaatgtgaagaacgttgatttgactagtgatatccagcag
ttcaaaatgaatatcgatcgtcaatcaatgtctgtgaaggagattgcagcaggttgtcaa
gtggagacggatttcacatacgagaagaggtcaaatttaatcaatatcatctccaagaag
gatctcagttgtcaaagaatcatgaagaagacggaagtttctgttccagtgaccaaagat
tccagaaacctgaagcgacatattctcgatgaagctccaactgttcaaaagaagcataag
ctggatgttttggaaaagtcaactgtggattccttgctctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAACATTGGAGTGGCGTGATTTGC 219 529 1066 Antisense
GAAGGAGGAGCTGGCATTCGGTGGA 55 335 1158 Antisense
GGCATTCGGTGGATTCTTCGAGTCA 404 535 1170 Antisense
GAATCCGGCAACTTGTGCAAATTTT 597 329 1196 Antisense
GGTTCATCGGATTGGAGTTTGTTGC 446 507 1301 Antisense
TGACTAGTGATATCCAGCAGTTCAA 345 571 1346 Antisense
ATCGATCGTCAATCAATGTCTGTGA 135 37 1378 Antisense
GAGATTGCAGCAGGTTGTCAAGTGG 318 397 1405 Antisense
GACGGAAGTTTCTGTTCCAGTGACC 237 375 1515 Antisense
GAAACCTGAAGCGACATATTCTCGA 430 359 1550 Antisense
AGTCAACTGTGGATTCCTTGCTCTC 626 61 1622 Antisense
Sequence Source Affymetrix Proprietary Database