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[189607_at] choline kinase

Description B0285.10 /REP_DB=WormBase Gene ID /WP=CE00649 /TR=SW:P46560 /GB=CAA84303.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=choline kinase
Cluster 6grp_total > 08      4grp_total > 36      6grp_cgc > 40      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 55.8
(-3)
A 		82.3
(0.5)
P 		59.5
(-2.5)
A 		101.2
(3)
A 		45
B 50.3
(0.3)
P 		77.3
(3)
A 		16.8
(-3)
A 		31.2
(-1.6)
A 		61
C 83.4
(3)
A 		66.5
(-2)
A 		64
(-2.7)
A 		63
(-3)
A 		20
avr 63.2
(0.4) 		75.4
(3) 		46.8
(-3) 		65.1
(0.8) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 80.5
(3)
A 		55.9
(0.5)
A 		74.1
(2.3)
A 		42.8
(-0.8)
A 		21.5
(-3)
A 		24.6
(-2.7)
A 		59
B 72.6
(3)
A 		55.8
(1.5)
A 		5.6
(-3)
A 		28.3
(-1)
A 		31.6
(-0.7)
A 		42.9
(0.3)
A 		67
C 81.4
(0.9)
A 		65.9
(-0.4)
A 		39.5
(-2.7)
A 		106.2
(3)
A 		42.9
(-2.4)
A 		36
(-3)
A 		70
avr 78.2
(3) 		59.2
(0.5) 		39.7
(-2) 		59.1
(0.5) 		32
(-3) 		34.5
(-2.7) 		46
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 20.3
(3)
A 		17.9
(0.4)
P 		15.6
(-2.1)
A 		14.8
(-3)
A 		6
B 16
(1.8)
P 		19.1
(3)
A 		3.9
(-3)
A 		6.7
(-1.9)
A 		15
C 27.6
(3)
A 		19.1
(0)
A 		22.3
(1.1)
A 		10.6
(-3)
A 		17
avr 21.3
(3) 		18.7
(1.5) 		13.9
(-1.2) 		10.7
(-3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 14.8
(0.8)
A 		19.9
(3)
A 		16.1
(1.4)
A 		10.9
(-0.9)
A 		6.5
(-2.8)
A 		6
(-3)
A 		14
B 25.3
(3)
A 		18.6
(1.3)
A 		1.4
(-3)
A 		7.5
(-1.5)
A 		12.8
(-0.1)
A 		16
(0.7)
A 		24
C 30.2
(3)
A 		20.1
(-0)
A 		10.1
(-3)
A 		21.4
(0.4)
A 		13.9
(-1.9)
A 		12.1
(-2.4)
A 		20
avr 23.4
(3) 		19.5
(1.4) 		9.2
(-3) 		13.3
(-1.3) 		11.1
(-2.2) 		11.4
(-2.1) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189607_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7978
Sequence Type Exemplar sequence
Representative Public ID B0285.10 NCBI
Target Description B0285.10 /REP_DB=WormBase Gene ID /WP=CE00649 /TR=SW:P46560 /GB=CAA84303.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=choline kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065480(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065480 NCBI Caenorhabditis elegans Choline Kinase B family member (ckb-3) (ckb-3) mRNA, complete cds. 11/11 None
B0285.10 ENSEMBL cdna:known chromosome:CEL140:III:4371115:4372789:1 gene:B0285.10 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000025279 4/11 Cross Hyb Matching Probes None
GENEFINDER00000025287 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4371114-4372789(+) 100.0 100.0
Public Domain and Genome References
Gene Title choline kinase
Gene Symbol ckb-3
Ensembl B0285.10
Entrez Gene 181905 Entrez gene
SwissProt P46560 EMBL-EBI
WormBase CE00649 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA84303 Hypothetical protein B0285.10 [Caenorhabditis elegans] emb|CAA83224.1| Hypothetical protein B0285.10 [Caenorhabditis elegans] ref|NP_497881.1| Choline Kinase B family member (ckb-3) [Caenorhabditis elegans] sp|P46560|KICB3_CAEEL Putative choline kinase B3 0.0
blast CAA84300 Hypothetical protein B0285.8 [Caenorhabditis elegans] ref|NP_497879.2| Choline Kinase B family member (ckb-1) [Caenorhabditis elegans] gb|AAN41642.1| choline kinase CKB-1 [Caenorhabditis elegans] sp|P46558|KICB1_CAEEL Choline kinase B1 1.0E-119
blast CAE57506 Hypothetical protein CBG00480 [Caenorhabditis briggsae] 1.0E-115
Protein Domains
Database ID Description E-Value
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:189607_AT
gtggcgactttgcaattttccccacggtggtcacctattcagaacacccaaaaagtattt
ctatcaaggatttgctaacagaaatcgatcttctagaaaaatggtcgatcgatttgttcg
agaatactttggttttctgtcacaatgatttgacgtcatccaacatccttcaactaaact
ccactggagaacttgtattcattgattgggaaaatgcatcctataactggagaggatatg
atttagcgatgcatttgtctgaagctgctgtaattcgaaacacttgcccgcctggaattg
tgataaatgaggagttaactgataatccacctaacctccaggcgttttgtgaagcttacg
tggattcggagaacaagatcaaaggccttctgtcatcaaatatttcatctcaagtgaatt
ccttgatccaagaatgtaaattcttctggcccataactcatctcttctgggcatgtttta
tcatgaaattgggattgtttgagtgtaatcgtgaaatcaaattgaatgctcaagctcgcg
aacatttggcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGGCGACTTTGCAATTTTCCCCAC 39 493 347 Antisense
GTGGTCACCTATTCAGAACACCCAA 615 489 373 Antisense
TGATTTGACGTCATCCAACATCCTT 658 563 492 Antisense
CATCCTTCAACTAAACTCCACTGGA 401 211 510 Antisense
ACACTTGCCCGCCTGGAATTGTGAT 526 109 626 Antisense
AACTGATAATCCACCTAACCTCCAG 258 141 663 Antisense
AACCTCCAGGCGTTTTGTGAAGCTT 183 147 679 Antisense
GATCAAAGGCCTTCTGTCATCAAAT 544 421 723 Antisense
AATTCTTCTGGCCCATAACTCATCT 319 177 785 Antisense
AACTCATCTCTTCTGGGCATGTTTT 89 141 801 Antisense
GCTCAAGCTCGCGAACATTTGGCAG 545 301 874 Antisense
Sequence Source Affymetrix Proprietary Database