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[189596_at] myosin

Description F56A6.2 /REP_DB=WormBase Gene ID /WP=CE23779 /GEN=hum-7 /TR=Q9GZG6 /GB=AAF99977.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=myosin
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 16      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 60      6grp_total > 16      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 13      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 346
1 2 3 4 range
A 547.7
(3)
P 		340.6
(-0.8)
P 		216.6
(-3)
P 		331.1
(-0.9)
P 		331
B 562.8
(3)
P 		348.9
(-2.2)
P 		320.6
(-2.9)
P 		315.8
(-3)
P 		247
C 522.6
(3)
P 		303.1
(-3)
P 		317.4
(-2.6)
P 		348.8
(-1.8)
P 		220
avr 544.4
(3) 		330.9
(-1.9) 		284.9
(-3) 		331.9
(-1.9) 		260

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 496
1 2 3 4 5 6 range
A 633.4
(3)
P 		403.5
(-0.2)
P 		201.3
(-3)
P 		252.5
(-2.3)
P 		215.2
(-2.8)
P 		254.7
(-2.3)
P 		432
B 625.7
(3)
P 		442.6
(0.6)
P 		220.2
(-2.4)
P 		176.4
(-3)
P 		212.5
(-2.5)
P 		210.4
(-2.5)
P 		449
C 466.6
(3)
P 		400.7
(1.8)
P 		314.7
(0.2)
P 		137.7
(-3)
P 		290
(-0.2)
P 		204.6
(-1.8)
P 		329
avr 575.2
(3) 		415.6
(0.5) 		245.4
(-2.1) 		188.9
(-3) 		239.2
(-2.2) 		223.2
(-2.5) 		386
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 151
1 2 3 4 range
A 199.4
(3)
P 		73.9
(-2)
P 		56.6
(-2.7)
P 		48.5
(-3)
P 		151
B 179.5
(3)
P 		86.2
(-2)
P 		74.3
(-2.6)
P 		67.3
(-3)
P 		112
C 173
(3)
P 		87.1
(-1.5)
P 		110.6
(-0.3)
P 		58.8
(-3)
P 		114
avr 184
(3) 		82.4
(-1.8) 		80.5
(-1.9) 		58.2
(-3) 		126

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 190
1 2 3 4 5 6 range
A 116.8
(1.4)
P 		143.5
(3)
P 		43.8
(-3)
P 		64
(-1.8)
P 		64.9
(-1.7)
P 		62
(-1.9)
P 		100
B 217.7
(3)
P 		147.3
(0.5)
P 		53.5
(-2.8)
P 		46.7
(-3)
P 		86.3
(-1.6)
P 		78.5
(-1.9)
P 		171
C 173
(3)
P 		122.3
(0.9)
P 		80.1
(-0.8)
P 		27.7
(-3)
P 		93.9
(-0.3)
P 		68.5
(-1.3)
P 		145
avr 169.2
(3) 		137.7
(1.5) 		59.1
(-2.4) 		46.1
(-3) 		81.7
(-1.3) 		69.7
(-1.8) 		123
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189596_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2577
Sequence Type Exemplar sequence
Representative Public ID F56A6.2 NCBI
Target Description F56A6.2 /REP_DB=WormBase Gene ID /WP=CE23779 /GEN=hum-7 /TR=Q9GZG6 /GB=AAF99977.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=myosin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058354(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058354 NCBI Caenorhabditis elegans F56A6.2 (myosin) mRNA, complete cds. 11/11 None
SNAP00000032358 ENSEMBL cdna:SNAP chromosome:CEL140:I:585863:596675:-1 11/11 None
GENEFINDER00000032368 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:585863:598674:-1 11/11 None
F56A6.2 ENSEMBL cdna:known chromosome:CEL140:I:585834:607574:-1 gene:F56A6.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:585862-607574(-) 100.0 100.0
Public Domain and Genome References
Gene Title myosin
Gene Symbol hum-7
Ensembl F56A6.2
Entrez Gene 171650 Entrez gene
SwissProt Q9GZG6 EMBL-EBI
WormBase CE23779 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.23841.1.S1_AT similar to myosin IXB cfa
HU35KSUBA:RC_AA029455_AT myosin IXB hs
HU35KSUBD:RC_N51705_AT myosin IXB hs
HG-U133A:208452_X_AT myosin IXB hs
HG-U133_PLUS_2:208452_X_AT myosin IXB hs
HG-U133A_2:208452_X_AT myosin IXB hs
U133_X3P:HS.159629.4.A1_3P_A_AT myosin IXB hs
U133_X3P:HS.159629.3.A1_3P_A_AT myosin IXB hs
HG-U133A_2:214780_S_AT myosin IXB hs
HG-FOCUS:214780_S_AT myosin IXB hs
HG-U133_PLUS_2:214780_S_AT myosin IXB hs
HG-U133A:214780_S_AT myosin IXB hs
HG-U133A_2:217297_S_AT myosin IXB hs
HG-U133_PLUS_2:217297_S_AT myosin IXB hs
HG-U133A:217297_S_AT myosin IXB hs
HG-U95AV2:33816_AT myosin IXB hs
HG-U95AV2:40539_AT myosin IXB hs
HG-U95D:72746_R_AT myosin IXB hs
HG-U133_PLUS_2:242742_AT Myosin IXB hs
HG-U133B:242742_AT Myosin IXB hs
HG-U95E:73750_AT Myosin IXB hs
U133_X3P:HS.123198.0.A1_3P_AT Myosin IXB hs
MG-U74BV2:114776_AT myosin IXb mm
MOE430A:1418031_AT myosin IXb mm
MOUSE430A_2:1418031_AT myosin IXb mm
MOUSE430_2:1418031_AT myosin IXb mm
MOE430B:1438533_AT myosin IXb mm
MOUSE430_2:1438533_AT myosin IXb mm
MOE430B:1459110_AT Myosin IXb (Myo9b), mRNA mm
MOUSE430_2:1459110_AT Myosin IXb (Myo9b), mRNA mm
MU19KSUBB:TC32715_AT Myosin IXb (Myo9b), mRNA mm
MU19KSUBC:TC37897_AT Myosin IXb (Myo9b), mRNA mm
RG-U34A:X77609_AT myosin IXb rn
RAE230A:1387866_AT myosin IXb rn
RAT230_2:1387866_AT myosin IXb rn
RAE230A:1375169_AT Myosin IXb rn
RAT230_2:1375169_AT Myosin IXb rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7165 signal transduction inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16459 myosin inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3774 motor activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF99977 Heavy chain, unconventional myosin protein 7 [Caenorhabditis elegans] ref|NP_490755.1| F56A6.2 [Caenorhabditis elegans] 0.0
blast T33079 hypothetical protein F56A6.2 - Caenorhabditis elegans 0.0
blast CAE60310 Hypothetical protein CBG03901 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000159 EMBL-EBI RA domain 3.7E-22
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 3.0E-10
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 1.0E-126
Pfam IPR000198 EMBL-EBI RhoGAP domain 6.0E-60
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 3.0E-10
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 3.5E-34
Pfam IPR000198 EMBL-EBI RhoGAP domain 6.0E-60
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 3.0E-10
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 2.4E-58
Pfam IPR000198 EMBL-EBI RhoGAP domain 6.0E-60
Sequence
Target Sequence 
>C. ELEGANS:189596_AT
gccgaccaggaagccgtcaacaaaatgggctgcaacaacttggctctgatcttcgggcca
tgtgtgctccgccgacaagatagtgctcatgctcaggagcagttgaatgacgtggcacgg
caaacgggttgtgttcagactttaattgaggagaagctgaaacagtataaagccaccatt
cataacattgttgagctggaggatgctagtcagaaggtatcagcaaacttgcgaaaaatt
gaagagcaccgtcggaatagtgaaccatcgaaattttcgccgaatattgggactgcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCGACCAGGAAGCCGTCAACAAAA 708 283 5062 Antisense
ACAAAATGGGCTGCAACAACTTGGC 634 107 5081 Antisense
TGCTCCGCCGACAAGATAGTGCTCA 541 581 5126 Antisense
GATAGTGCTCATGCTCAGGAGCAGT 272 425 5140 Antisense
GAGCAGTTGAATGACGTGGCACGGC 123 385 5158 Antisense
GTGGCACGGCAAACGGGTTGTGTTC 432 415 5173 Antisense
ACGGGTTGTGTTCAGACTTTAATTG 601 93 5185 Antisense
AAAGCCACCATTCATAACATTGTTG 263 125 5230 Antisense
GTTGAGCTGGAGGATGCTAGTCAGA 205 437 5251 Antisense
GAGCACCGTCGGAATAGTGAACCAT 546 387 5305 Antisense
TTTCGCCGAATATTGGGACTGCAAA 439 667 5336 Antisense
Sequence Source Affymetrix Proprietary Database