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[189591_s_at] ATPase subunit

Description T14F9.1 /REP_DB=WormBase Gene ID /WP=CE07497 /TR=SW:Q22494 /GB=AAA91266.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=ATPase subunit
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 13      4grp_highest_8_8 > 33      4grp_high_8_8 > 50      6grp_highest_nonorm > 13      6grp_total > 23      4grp_total > 61      6grp_highest_sub > De      6grp_cgc > 30      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2911
1 2 3 4 range
A 4176.6
(3)
P 		3079.9
(0.1)
P 		4101.8
(2.8)
P 		1930.9
(-3)
P 		2246
B 4774
(3)
P 		3793.5
(0.8)
P 		3496.1
(0.2)
P 		2055.8
(-3)
P 		2718
C 4237.8
(3)
P 		4028.6
(2.5)
P 		3613.7
(1.4)
P 		1862.9
(-3)
P 		2375
avr 4396.1
(3) 		3634
(1.1) 		3737.2
(1.4) 		1949.9
(-3) 		2446

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2074
1 2 3 4 5 6 range
A 3300.1
(2.1)
P 		3608.1
(3)
P 		2498.4
(-0.2)
P 		2097.1
(-1.4)
P 		1845.6
(-2.1)
P 		1533.7
(-3)
P 		2074
B 3414.5
(2.6)
P 		3563.5
(3)
P 		3447.2
(2.7)
P 		2598.3
(0.1)
P 		1697.4
(-2.6)
P 		1552.4
(-3)
P 		2011
C 3564.4
(3)
P 		3386.3
(2.5)
P 		2954.7
(1.1)
P 		2598.5
(0)
P 		1751.5
(-2.6)
P 		1615.3
(-3)
P 		1949
avr 3426.3
(2.7) 		3519.3
(3) 		2966.8
(1.3) 		2431.3
(-0.3) 		1764.8
(-2.4) 		1567.1
(-3) 		1952
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1240
1 2 3 4 range
A 1520.7
(3)
P 		668.6
(-1.1)
P 		1072.3
(0.8)
P 		282.6
(-3)
P 		1238
B 1522.4
(3)
P 		936.9
(-0.2)
P 		810.1
(-0.9)
P 		438.1
(-3)
P 		1084
C 1402.8
(3)
P 		1158.2
(1.7)
P 		1259.5
(2.2)
P 		314.1
(-3)
P 		1089
avr 1482
(3) 		921.2
(0) 		1047.3
(0.7) 		344.9
(-3) 		1137

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 948
1 2 3 4 5 6 range
A 608.4
(-1.5)
P 		1283.2
(3)
P 		544.1
(-1.9)
P 		531.9
(-2)
P 		556.9
(-1.8)
P 		373.4
(-3)
P 		910
B 1188.1
(3)
P 		1186.2
(3)
P 		837.1
(-0.5)
P 		688.2
(-1.9)
P 		689.6
(-1.9)
P 		578.9
(-3)
P 		609
C 1321.2
(3)
P 		1033.3
(0.8)
P 		752
(-1.3)
P 		522.6
(-3)
P 		566.8
(-2.7)
P 		540.7
(-2.9)
P 		799
avr 1039.2
(1.8) 		1167.6
(3) 		711.1
(-1.1) 		580.9
(-2.3) 		604.4
(-2) 		497.7
(-3) 		670
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189591_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18116
Sequence Type Exemplar sequence
Representative Public ID T14F9.1 NCBI
Target Description T14F9.1 /REP_DB=WormBase Gene ID /WP=CE07497 /TR=SW:Q22494 /GB=AAA91266.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=ATPase subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076011(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076011 NCBI Caenorhabditis elegans Vacuolar H ATPase family member (vha-15) (vha-15) mRNA, complete cds. 11/11 None
SNAP00000024571 ENSEMBL cdna:SNAP chromosome:CEL140:X:2237906:2239896:1 11/11 None
GENEFINDER00000024576 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2237906:2239896:1 11/11 None
T14F9.1.3 ENSEMBL cdna:known chromosome:CEL140:X:2237902:2240248:1 gene:T14F9.1 11/11 None
T14F9.1.2 ENSEMBL cdna:known chromosome:CEL140:X:2237902:2240248:1 gene:T14F9.1 11/11 None
T14F9.1.1 ENSEMBL cdna:known chromosome:CEL140:X:2237904:2240248:1 gene:T14F9.1 11/11 None
T14F9.1.4 ENSEMBL cdna:known chromosome:CEL140:X:2237906:2240230:1 gene:T14F9.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2237905-2239896(+) 100.0 100.0
Public Domain and Genome References
Gene Title ATPase subunit
Gene Symbol vha-15
Ensembl T14F9.1
Entrez Gene 180534 Entrez gene
SwissProt Q22494 EMBL-EBI
WormBase CE07497 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:252845_AT vacuolar ATP synthase subunit H family protein at
CANINE_2:CFA.20869.1.S1_S_AT similar to ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H cfa
CANINE_2:CFAAFFX.11312.1.S1_S_AT similar to ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H cfa
DROSOPHILA_2:1633931_A_AT Vacuolar H+-ATPase SFD subunit dm
DROSGENOME1:148646_AT Vacuolar H+-ATPase SFD subunit dm
DROSGENOME1:144191_AT Vacuolar H+-ATPase SFD subunit dm
CHICKEN:GGAAFFX.8679.1.S1_S_AT similar to 54 kDa vacuolar H(+)-ATPase subunit gga
HG-U95AV2:33742_F_AT ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H hs
HG-U133A:221504_S_AT ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H hs
HG-U133_PLUS_2:221504_S_AT ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H hs
HG-U133A_2:221504_S_AT ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H hs
HU35KSUBB:RC_Z39349_AT ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H hs
U133_X3P:G6563195_3P_S_AT ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H hs
U133_X3P:HS2.371561.1.A1_3P_AT ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H hs
HG-U133_PLUS_2:1557585_AT ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H hs
HG-U133_PLUS_2:1557586_S_AT ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H hs
HG-U95AV2:33741_AT ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H hs
HG-U95E:89225_AT ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H hs
HU35KSUBC:RC_N64368_AT ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H hs
MOUSE430_2:1415826_AT ATPase, H+ transporting, lysosomal, V1 subunit H mm
MOUSE430A_2:1415826_AT ATPase, H+ transporting, lysosomal, V1 subunit H mm
MOE430A:1415826_AT ATPase, H+ transporting, lysosomal, V1 subunit H mm
MG-U74AV2:96610_AT ATPase, H+ transporting, lysosomal, V1 subunit H mm
MG-U74BV2:165108_R_AT ATPase, H+ transporting, lysosomal, V1 subunit H mm
MG-U74CV2:166851_I_AT ATPase, H+ transporting, lysosomal, V1 subunit H mm
MOE430B:1457639_AT ATPase, H+ transporting, lysosomal, V1 subunit H mm
MOUSE430_2:1457639_AT ATPase, H+ transporting, lysosomal, V1 subunit H mm
MOE430B:1447659_X_AT ATPase, H+ transporting, lysosomal, V1 subunit H mm
MOUSE430_2:1447659_X_AT ATPase, H+ transporting, lysosomal, V1 subunit H mm
MU19KSUBB:TC32167_AT ATPase, H+ transporting, lysosomal, V1 subunit H (Atp6v1h), mRNA mm
RG-U34B:RC_AA942681_AT vacuolar ATPase subunit H rn
RAE230B:1379730_AT vacuolar ATPase subunit H rn
RAT230_2:1379730_AT vacuolar ATPase subunit H rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
15986 ATP synthesis coupled proton transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16469 proton-transporting two-sector ATPase complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
46933 hydrogen-transporting ATP synthase activity, rotational mechanism inferred from electronic annotation QuickGO AmiGO
46961 hydrogen-transporting ATPase activity, rotational mechanism inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA91266 Vacuolar h atpase protein 15 [Caenorhabditis elegans] ref|NP_508412.1| Vacuolar H ATPase family member (vha-15) [Caenorhabditis elegans] sp|Q22494|VATH2_CAEEL Probable vacuolar ATP synthase subunit H 2 (V-ATPase H subunit 2) (Vacuolar proton pump H subunit 2) (Vacuolar proton pump subunit SFD 2) 0.0
blast CAE68335 Hypothetical protein CBG14055 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004908 EMBL-EBI V-ATPase subunit H 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:189591_S_AT
atcagaatcattctcgcttctttcgtgaacattttgagcaaggttgatgagcgtgaagtc
aagagggaggctgctctccagatggttcaatgcaaaactttgaagactttggagctgatg
gatgctaagaaatacgacgacccagatcttgaggatgatgtcaagttcctcactgaagag
ctgaccctttcagtgcacgacttgagctcctacgatgagtattacagcgaggttcgttcc
ggacgtcttcaatggtctccagtgcacaagtcagagaagttctggcgtgaaaacgcttcc
aagttcaatgacaagcaattcgaagttgtcaagatccttatcaagcttcttgaatctagt
catgacccactgatcctttgtgttgcttctcatgatatcggagagtatgttcgtcattac
ccgagaggaaagaccgtcgtcgagcagtatcaaggtaaggcagctgttatgcgtctcctg
actgccgaggatccaaatgttcgctaccatgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCAGAATCATTCTCGCTTCTTTCG 324 29 817 Antisense
GAAATACGACGACCCAGATCTTGAG 656 361 945 Antisense
AGTTCCTCACTGAAGAGCTGACCCT 196 65 980 Antisense
TTTCAGTGCACGACTTGAGCTCCTA 163 671 1004 Antisense
TATTACAGCGAGGTTCGTTCCGGAC 709 657 1036 Antisense
GTCTTCAATGGTCTCCAGTGCACAA 317 469 1061 Antisense
CATGACCCACTGATCCTTTGTGTTG 317 209 1177 Antisense
TTGTGTTGCTTCTCATGATATCGGA 536 707 1194 Antisense
GAGAGTATGTTCGTCATTACCCGAG 615 393 1217 Antisense
TAAGGCAGCTGTTATGCGTCTCCTG 707 635 1272 Antisense
AGGATCCAAATGTTCGCTACCATGC 319 55 1304 Antisense
Sequence Source Affymetrix Proprietary Database