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[189586_s_at] tubulin beta-chain

Description K01G5.7 /REP_DB=WormBase Gene ID /WP=CE16197 /TR=O17921 /GB=CAB07246.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=tubulin beta-chain
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 12      4grp_highest_8_8 > 16      4grp_high_8_8 > 52      6grp_highest_nonorm > 16      6grp_total > 33      4grp_total > 59      6grp_highest_sub > De      6grp_cgc > 21      6grp_high1_sub > De      6grp_cgc_sub > De      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10562
1 2 3 4 range
A 11350.3
(0.8)
P 		15017.9
(3)
P 		14080.7
(2.4)
P 		5108.2
(-3)
P 		9910
B 10654
(0.6)
P 		14513.6
(3)
P 		14312.8
(2.9)
P 		4873.6
(-3)
P 		9640
C 10629.1
(1)
P 		13618.7
(3)
P 		12257.9
(2.1)
P 		4455.6
(-3)
P 		9163
avr 10877.8
(0.8) 		14383.4
(3) 		13550.5
(2.5) 		4812.5
(-3) 		9571

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4702
1 2 3 4 5 6 range
A 9369.4
(3)
P 		8205.4
(0.8)
P 		7771.1
(-0)
P 		6384
(-2.6)
P 		6182.8
(-3)
P 		6702.3
(-2)
P 		3187
B 8204.7
(3)
P 		7298.3
(0.5)
P 		8036.2
(2.5)
P 		6958.3
(-0.4)
P 		6030.2
(-3)
P 		6079.8
(-2.9)
P 		2175
C 8070.4
(2.5)
P 		7566.9
(1.7)
P 		8399.1
(3)
P 		6390.5
(-0.2)
P 		6621.1
(0.1)
P 		4667
(-3)
P 		3732
avr 8548.2
(3) 		7690.2
(1.1) 		8068.8
(1.9) 		6577.6
(-1.3) 		6278
(-2) 		5816.4
(-3) 		2732
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3525
1 2 3 4 range
A 4132.7
(3)
P 		3260
(1.5)
P 		3681
(2.2)
P 		747.7
(-3)
P 		3385
B 3397.5
(2.6)
P 		3584.5
(3)
P 		3316.6
(2.4)
P 		1038.6
(-3)
P 		2546
C 3518.5
(1.7)
P 		3915.5
(2.4)
P 		4272.2
(3)
P 		751.3
(-3)
P 		3521
avr 3682.9
(2.8) 		3586.7
(2.6) 		3756.6
(3) 		845.9
(-3) 		2911

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1706
1 2 3 4 5 6 range
A 1727.2
(-2.5)
P 		2918.3
(3)
P 		1692.4
(-2.7)
P 		1619.1
(-3)
P 		1865.7
(-1.9)
P 		1631.8
(-2.9)
P 		1299
B 2854.8
(3)
P 		2429.3
(0.5)
P 		1951.4
(-2.4)
P 		1843
(-3)
P 		2450
(0.6)
P 		2267.1
(-0.5)
P 		1012
C 2991.4
(3)
P 		2308.9
(0.6)
P 		2137.7
(-0)
P 		1285.1
(-3)
P 		2142.5
(0)
P 		1562.3
(-2)
P 		1706
avr 2524.5
(2.8) 		2552.2
(3) 		1927.2
(-0.9) 		1582.4
(-3) 		2152.7
(0.5) 		1820.4
(-1.5) 		970
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189586_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7671
Sequence Type Exemplar sequence
Representative Public ID K01G5.7 NCBI
Target Description K01G5.7 /REP_DB=WormBase Gene ID /WP=CE16197 /TR=O17921 /GB=CAB07246.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=tubulin beta-chain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066966(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066966 NCBI Caenorhabditis elegans Tubulin, Beta family member (tbb-1) (tbb-1) mRNA, complete cds. 11/11 None
SNAP00000033486 ENSEMBL cdna:SNAP chromosome:CEL140:III:10740858:10742302:-1 11/11 None
GENEFINDER00000033496 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10740858:10742302:-1 11/11 None
K01G5.7.1 ENSEMBL cdna:known chromosome:CEL140:III:10740751:10742309:-1 gene:K01G5.7 11/11 None
K01G5.7.2 ENSEMBL cdna:known chromosome:CEL140:III:10740754:10742327:-1 gene:K01G5.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10740853-10742298(-) 100.0 100.0
Public Domain and Genome References
Gene Title tubulin beta-chain
Gene Symbol tbb-1
Ensembl K01G5.7
Entrez Gene 176501 Entrez gene
SwissProt O17921 EMBL-EBI
WormBase CE16197 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:16051_AT tubulin beta-9 chain (TUB9) at
ATH1-121501:254446_AT tubulin beta-9 chain (TUB9) at
HG-U95AV2:429_F_AT tubulin, beta 4 hs
HC-G110:429_F_AT tubulin, beta 4 hs
HU35KSUBD:RC_AA432380_S_AT tubulin, beta 4 hs
HUGENEFL:X00734_AT tubulin, beta 4 hs
U133_X3P:HS.110837.0.S2_3P_AT tubulin, beta 4 hs
HG-U133A_2:212664_AT tubulin, beta 4 hs
HG-FOCUS:212664_AT tubulin, beta 4 hs
HG-U133_PLUS_2:212664_AT tubulin, beta 4 hs
HG-U133A:212664_AT tubulin, beta 4 hs
HG-U95AV2:38699_AT tubulin, beta 4 hs
MU11KSUBB:MSA.17028.0_F_AT tubulin, beta 4 mm
MU11KSUBB:MSA.1262.0_S_AT tubulin, beta 4 mm
MG-U74AV2:101419_AT tubulin, beta 4 mm
MOE430A:1423221_AT tubulin, beta 4 mm
MOUSE430A_2:1423221_AT tubulin, beta 4 mm
MOUSE430_2:1423221_AT tubulin, beta 4 mm
MU11KSUBA:M28730_RC_AT tubulin, beta 4 mm
MG-U74BV2:105113_AT Tubulin, beta 4 (Tubb4), mRNA mm
MU19KSUBA:TC25539_AT Tubulin, beta 4 (Tubb4), mRNA mm
MU19KSUBB:TC30844_AT Tubulin, beta 4 (Tubb4), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7018 microtubule-based movement inferred from electronic annotation QuickGO AmiGO
46785 microtubule polymerization inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5874 microtubule inferred from electronic annotation QuickGO AmiGO
45298 tubulin inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3924 GTPase activity inferred from electronic annotation QuickGO AmiGO
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07246 Hypothetical protein K01G5.7 [Caenorhabditis elegans] ref|NP_499367.1| Tubulin, Beta family member (tbb-1) [Caenorhabditis elegans] 0.0
blast CAE71409 Hypothetical protein CBG18319 [Caenorhabditis briggsae] 0.0
blast CAA84648 Hypothetical protein C36E8.5 [Caenorhabditis elegans] ref|NP_497806.1| Tubulin, Beta family member (tbb-2) [Caenorhabditis elegans] sp|P52275|TBB2_CAEEL Tubulin beta-2 chain (Beta-2 tubulin) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 1.2E-112
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 1.8E-79
Sequence
Target Sequence 
>C. ELEGANS:189586_S_AT
gcttccctggacagctgaacgctgatcttcgcaagttggctgtcaacatggttccattcc
cacgtcttcatttcttcatgcctggattcgctccactctccgccaagggagctcaggctt
accgtgcattgactgtcgccgagcttactcaacaaatgttcgacgccaagaacatgatgg
ctgcttgcgatccaagacacggaagatacctcaccgttgctgctatgttccgtggacgca
tgtccatgagagaggttgacgagcagatgctcagtgtccagaacaagaactcctcgtact
tcgttgaatggattccaaacaacgtcaagactgctgtgtgcgacatcccaccacgtggac
tcaagatggctgccaccttcgtcggaaactccactgctatccaggagctcttcaagcgta
tctcggagcaattcaccgccatgttccgcagaaaggccttccttcattggtacactggtg
agggtatggacgagatggagttcaccgaggctgaaagcaacatgaacgacttgatctcgg
agtaccaacaataccaggaagctaccgccgaagacgagccactcgacgagttcgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTTCCCTGGACAGCTGAACGCTGA 21 307 734 Antisense
GAACGCTGATCTTCGCAAGTTGGCT 132 347 750 Antisense
TCATTTCTTCATGCCTGGATTCGCT 71 621 801 Antisense
GGCTTACCGTGCATTGACTGTCGCC 371 537 849 Antisense
CTGTCGCCGAGCTTACTCAACAAAT 473 239 866 Antisense
AACTCCACTGCTATCCAGGAGCTCT 644 141 1120 Antisense
CAGGAGCTCTTCAAGCGTATCTCGG 268 209 1135 Antisense
GTATCTCGGAGCAATTCACCGCCAT 268 455 1151 Antisense
TCACCGCCATGTTCCGCAGAAAGGC 612 625 1166 Antisense
CAATACCAGGAAGCTACCGCCGAAG 557 179 1282 Antisense
AGACGAGCCACTCGACGAGTTCGCC 487 71 1305 Antisense
Sequence Source Affymetrix Proprietary Database