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[189584_s_at] glycolate oxidase

Description F41E6.5 /REP_DB=WormBase Gene ID /WP=CE10252 /TR=O16457 /GB=AAB65955.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=glycolate oxidase
Cluster 6grp_highest_10_10 > 38      6grp_high1_10_10 > 72      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 54      6grp_total > 73      4grp_total > 39      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 322
1 2 3 4 range
A 842.5
(3)
P 		764
(0.6)
P 		646.9
(-3)
P 		690.4
(-1.7)
P 		196
B 897.7
(3)
P 		624.3
(-3)
P 		683.6
(-1.7)
P 		659.7
(-2.2)
P 		273
C 940.4
(3)
P 		618.8
(-3)
P 		698
(-1.5)
P 		882.6
(1.9)
P 		322
avr 893.5
(3) 		669
(-3) 		676.2
(-2.8) 		744.2
(-1) 		225

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 856
1 2 3 4 5 6 range
A 681.3
(-3)
P 		1188.8
(0.7)
P 		1355.5
(1.9)
P 		1512.1
(3)
P 		1174.3
(0.6)
P 		1206.4
(0.8)
P 		831
B 717.7
(-3)
P 		1013.2
(-0.7)
P 		1476.3
(3)
P 		1319.2
(1.8)
P 		1394.2
(2.4)
P 		1348.8
(2)
P 		759
C 818.5
(-3)
P 		1087.7
(-0.8)
P 		1526.2
(2.9)
P 		1537.7
(3)
P 		1150
(-0.2)
P 		980.5
(-1.6)
P 		719
avr 739.2
(-3) 		1096.6
(-0) 		1452.7
(3) 		1456.3
(3) 		1239.5
(1.2) 		1178.6
(0.7) 		717
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 210
1 2 3 4 range
A 306.7
(3)
P 		165.8
(-1.1)
P 		169.1
(-1)
P 		101.1
(-3)
P 		206
B 286.3
(3)
P 		154.2
(-2.4)
P 		158.4
(-2.3)
P 		140.6
(-3)
P 		146
C 311.3
(3)
P 		177.9
(-1.9)
P 		243.3
(0.5)
P 		148.8
(-3)
P 		163
avr 301.4
(3) 		166
(-1.7) 		190.3
(-0.9) 		130.2
(-3) 		171

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 441
1 2 3 4 5 6 range
A 125.6
(-3)
P 		422.8
(3)
P 		295.2
(0.4)
P 		383.5
(2.2)
P 		354.4
(1.6)
P 		293.7
(0.4)
P 		297
B 249.7
(-3)
P 		337.3
(-1.3)
P 		358.5
(-0.9)
P 		349.4
(-1.1)
P 		566.4
(3)
P 		503
(1.8)
P 		317
C 303.4
(-3)
P 		331.9
(-1)
P 		388.4
(3)
P 		309.2
(-2.6)
P 		372.1
(1.8)
P 		328.2
(-1.2)
P 		85
avr 226.2
(-3) 		364
(1) 		347.4
(0.6) 		347.4
(0.6) 		431
(3) 		375
(1.4) 		205
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189584_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12304
Sequence Type Exemplar sequence
Representative Public ID F41E6.5 NCBI
Target Description F41E6.5 /REP_DB=WormBase Gene ID /WP=CE10252 /TR=O16457 /GB=AAB65955.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=glycolate oxidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072817(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072817 NCBI Caenorhabditis elegans F41E6.5 (F41E6.5) mRNA, complete cds. 11/11 None
SNAP00000009423 ENSEMBL cdna:SNAP chromosome:CEL140:V:8606183:8607668:1 11/11 None
GENEFINDER00000009438 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8606183:8607668:1 11/11 None
F41E6.5 ENSEMBL cdna:known chromosome:CEL140:V:8606156:8608004:1 gene:F41E6.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
F41E6.9 11/11 Negative Strand Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8606183-8607669(+) 100.0 100.0
Public Domain and Genome References
Gene Title glycolate oxidase
Ensembl F41E6.5
Entrez Gene 179241 Entrez gene
SwissProt O16457 EMBL-EBI
WormBase CE31936 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB65955 Hypothetical protein F41E6.5 [Caenorhabditis elegans] ref|NP_505218.2| F41E6.5 [Caenorhabditis elegans] 0.0
blast CAE58356 Hypothetical protein CBG01477 [Caenorhabditis briggsae] 0.0
blast AAB65955 Hypothetical protein F41E6.5 [Caenorhabditis elegans] ref|NP_505218.2| F41E6.5 [Caenorhabditis elegans] 1.0E-154
blast CAE58356 Hypothetical protein CBG01477 [Caenorhabditis briggsae] 1.0E-137
blast XP_230613 PREDICTED: similar to glycolate oxidase; short-chain alpha-hydroxy acid oxidase [Rattus norvegicus] 4.0E-92
Protein Domains
Database ID Description E-Value
Pfam IPR000262 EMBL-EBI FMN-dependent alpha-hydroxy acid dehydrogenase 3.0E-103
Pfam IPR000262 EMBL-EBI FMN-dependent alpha-hydroxy acid dehydrogenase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:189584_S_AT
gatactcctgttttgggacgacgtctaaaggatacctacaataagttttcgctcccaaaa
catctcaaattcgcgaatttcgagtcgaatacgcaggctgagatgccaaagggacatgtt
ggcgaatctggattcatgcaatacgtcagctctcaaatcgatccatcacttgattggaat
actttgaaatggatcagaaccaaaactaatctgccagttatagtaaaaggtgttatgaga
ggagatgatgctctcttagcacttgaagcaggagttgatggaataattgtttcgaatcat
ggtggacgtcagatggattgtacagtggctacaattgaatctctaccagaagttctccga
gcagttgacaatcgaattcctgtttggatggatggtggcgtacgaaatggacgagatatt
ctgaaagctgtggctctcggagcacgaggtgttttcgtgggacgtcctgtcctctgggga
cttgccacatcgggttccgctggtgtgtcagctgttcttggactacttcagtctgaattc
taccacgcgctgcaactttcgggattcaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATACTCCTGTTTTGGGACGACGTC 235 423 502 Antisense
TACCTACAATAAGTTTTCGCTCCCA 29 651 534 Antisense
GGGACATGTTGGCGAATCTGGATTC 703 493 612 Antisense
GGATTCATGCAATACGTCAGCTCTC 561 509 631 Antisense
ATACGTCAGCTCTCAAATCGATCCA 450 23 642 Antisense
GAGATGATGCTCTCTTAGCACTTGA 693 395 743 Antisense
GAAGTTCTCCGAGCAGTTGACAATC 426 337 850 Antisense
AAAGCTGTGGCTCTCGGAGCACGAG 300 123 925 Antisense
GGTGTGTCAGCTGTTCTTGGACTAC 625 499 1003 Antisense
ACTTCAGTCTGAATTCTACCACGCG 574 103 1026 Antisense
CGCGCTGCAACTTTCGGGATTCAGA 179 255 1047 Antisense
Sequence Source Affymetrix Proprietary Database