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[189583_at] Annexin

Description C28A5.3 /REP_DB=WormBase Gene ID /WP=CE00661 /GEN=nex-3 /TR=Q27473 /GB=CAA83598.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Annexin
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 19      4grp_highest_8_8 > 09      4grp_high_8_8 > 53      6grp_highest_nonorm > 60      6grp_total > 05      4grp_total > 58      6grp_cgc > 03      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 352
1 2 3 4 range
A 207
(-1.1)
P 		376.6
(2.9)
P 		382.2
(3)
P 		122.7
(-3)
P 		260
B 218.2
(0.3)
P 		352.4
(3)
P 		299.9
(1.9)
P 		57.5
(-3)
P 		295
C 251.6
(0.3)
P 		384.3
(2.6)
P 		409
(3)
P 		64.9
(-3)
P 		344
avr 225.6
(-0) 		371.1
(3) 		363.7
(2.8) 		81.7
(-3) 		289

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 291
1 2 3 4 5 6 range
A 378.6
(3)
P 		368.2
(2.5)
P 		315.6
(0.1)
P 		281.2
(-1.5)
P 		338.6
(1.2)
P 		248.1
(-3)
P 		131
B 530.3
(3)
P 		462.5
(1.6)
P 		238.9
(-3)
P 		261.7
(-2.5)
P 		491.7
(2.2)
P 		285.3
(-2)
P 		291
C 508.1
(3)
P 		446.5
(1.5)
P 		360.2
(-0.5)
P 		315.4
(-1.6)
P 		335
(-1.1)
P 		257.1
(-3)
P 		251
avr 472.3
(3) 		425.7
(1.7) 		304.9
(-1.8) 		286.1
(-2.4) 		388.4
(0.6) 		263.5
(-3) 		209
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 132
1 2 3 4 range
A 75.4
(1.2)
P 		81.7
(1.7)
P 		99.9
(3)
P 		18
(-3)
P 		82
B 69.6
(1.6)
P 		87
(3)
P 		69.5
(1.6)
P 		12.2
(-3)
P 		75
C 83.3
(0.3)
P 		110.5
(1.5)
P 		142.5
(3)
P 		10.9
(-3)
P 		132
avr 76.1
(1.1) 		93.1
(2.3) 		104
(3) 		13.7
(-3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 142
1 2 3 4 5 6 range
A 69.8
(-2.2)
P 		130.9
(3)
P 		68.7
(-2.3)
P 		71.3
(-2.1)
P 		102.2
(0.6)
P 		60.4
(-3)
P 		71
B 184.5
(2.4)
P 		153.9
(1.1)
P 		58
(-3)
P 		69.3
(-2.5)
P 		199.8
(3)
P 		106.4
(-1)
P 		142
C 188.3
(3)
P 		136.3
(0.5)
P 		91.7
(-1.6)
P 		63.4
(-3)
P 		108.4
(-0.8)
P 		86.1
(-1.9)
P 		125
avr 147.5
(3) 		140.4
(2.5) 		72.8
(-2.6) 		68
(-3) 		136.8
(2.2) 		84.3
(-1.8) 		80
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189583_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6401
Sequence Type Exemplar sequence
Representative Public ID C28A5.3 NCBI
Target Description C28A5.3 /REP_DB=WormBase Gene ID /WP=CE00661 /GEN=nex-3 /TR=Q27473 /GB=CAA83598.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Annexin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065502(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065502 NCBI Caenorhabditis elegans C28A5.3 (Annexin) mRNA, complete cds. 11/11 None
SNAP00000011689 ENSEMBL cdna:SNAP chromosome:CEL140:III:4442062:4444088:1 11/11 None
GENEFINDER00000011696 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4442062:4444088:1 11/11 None
C28A5.3 ENSEMBL cdna:known chromosome:CEL140:III:4442050:4444088:1 gene:C28A5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4442061-4444088(+) 100.0 100.0
Public Domain and Genome References
Gene Title Annexin
Gene Symbol nex-3
Ensembl C28A5.3
Entrez Gene 191716 Entrez gene
SwissProt Q27473 EMBL-EBI
WormBase CE00661 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
50819 negative regulation of coagulation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
5544 calcium-dependent phospholipid binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA83598 Hypothetical protein C28A5.3 [Caenorhabditis elegans] ref|NP_497903.1| C28A5.3 [Caenorhabditis elegans] 1.0E-176
blast CAE71201 Hypothetical protein CBG18063 [Caenorhabditis briggsae] 1.0E-163
blast AAB52702 Annexin family protein 1 [Caenorhabditis elegans] ref|NP_498109.1| anNEXin family member (nex-1) [Caenorhabditis elegans] gb|AAA99775.1| NEX1 annexin 2.0E-56
Protein Domains
Database ID Description E-Value
Pfam IPR001464 EMBL-EBI Annexin 8.1E-20
Pfam IPR001464 EMBL-EBI Annexin 1.2E-25
Pfam IPR001464 EMBL-EBI Annexin 8.6E-13
Pfam IPR001464 EMBL-EBI Annexin 2.5E-21
Sequence
Target Sequence 
>C. ELEGANS:189583_AT
aacacttacttcatgacttactccaaatcattggaagatgcaatttctgctgatacttct
ggagacttccggcgtcttctcattgtcattctccaagcaaaacgcgaagaaggtggattc
gctgatacattcaaagttgggcctcacgctgctcaaattctgaaaagttttgacaaaaag
tctggagtcgaaaaattcgatgcgttcaagatctttgcaacatgcaatggagctcatatt
caaaaagttattgaagaagtcgagagacaatctggaaaagagttccagaaacttattgac
aaggagctcagcggagatttcaagaatttggtgttgtctcttattgagacttcgagaaat
aagcctcgatattttgcaaattcgattcatttggcaaccaagggaatgggaacacgtgac
aaggatcttattcgtattctggtatcccgatcggaaaatgatttggtgatcattgaacac
gaatttcaaactctttttggaaaaccattgactcagctaatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACACTTACTTCATGACTTACTCCA 167 135 376 Antisense
TGCAATTTCTGCTGATACTTCTGGA 166 577 414 Antisense
TGATACTTCTGGAGACTTCCGGCGT 368 565 426 Antisense
TTGTCATTCTCCAAGCAAAACGCGA 541 707 458 Antisense
TACATTCAAAGTTGGGCCTCACGCT 227 645 501 Antisense
CCTCACGCTGCTCAAATTCTGAAAA 48 269 517 Antisense
GATGCGTTCAAGATCTTTGCAACAT 474 413 574 Antisense
GAATTTGGTGTTGTCTCTTATTGAG 391 325 699 Antisense
GACAAGGATCTTATTCGTATTCTGG 225 363 793 Antisense
TCGTATTCTGGTATCCCGATCGGAA 182 321 807 Antisense
GGAAAACCATTGACTCAGCTAATCA 592 527 874 Antisense
Sequence Source Affymetrix Proprietary Database