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[189577_at] cytochrome b5 like

Description F58B4.2 /REP_DB=WormBase Gene ID /WP=CE06012 /TR=Q20976 /GB=CAA98498.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome b5 like
Cluster 6grp_total > 33      4grp_total > 36      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 62.5
(2.2)
A 		53
(-3)
A 		55.9
(-1.4)
A 		63.9
(3)
A 		11
B 65
(-1.8)
A 		98
(3)
A 		56.8
(-3)
A 		74.4
(-0.4)
P 		41
C 81.6
(2.9)
P 		80.6
(2.8)
A 		29.3
(-3)
A 		82.6
(3)
P 		53
avr 69.7
(1.5) 		77.2
(3) 		47.3
(-3) 		73.6
(2.3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 68.5
(0.7)
A 		38.5
(-2)
A 		77.9
(1.5)
A 		27.9
(-3)
A 		94.1
(3)
A 		44.5
(-1.5)
A 		66
B 68.6
(2.7)
A 		71.7
(3)
A 		60.2
(1.8)
A 		45.6
(0.2)
A 		34
(-1)
A 		15
(-3)
A 		57
C 78.4
(3)
A 		69.4
(2)
A 		73
(2.4)
A 		42.2
(-1.1)
A 		45.3
(-0.8)
A 		25.7
(-3)
A 		53
avr 71.8
(3) 		59.9
(1.4) 		70.4
(2.8) 		38.6
(-1.6) 		57.8
(1.1) 		28.4
(-3) 		43
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 22.8
(3)
A 		11.5
(-2)
A 		14.6
(-0.6)
A 		9.3
(-3)
A 		14
B 20.7
(1.1)
A 		24.2
(3)
A 		13.2
(-3)
A 		15.8
(-1.6)
P 		11
C 27
(3)
P 		23.2
(1.6)
A 		10.2
(-3)
A 		13.9
(-1.7)
P 		17
avr 23.5
(3) 		19.6
(0.8) 		12.7
(-3) 		13
(-2.8) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 12.6
(-1.5)
A 		13.7
(-1.1)
A 		17
(-0.2)
A 		7.1
(-3)
A 		28.4
(3)
A 		10.8
(-2)
A 		21
B 23.9
(3)
A 		23.9
(3)
A 		14.6
(-0)
A 		12.1
(-0.9)
A 		13.8
(-0.3)
A 		5.6
(-3)
A 		18
C 29.1
(3)
A 		21.2
(0.7)
A 		18.6
(-0.1)
A 		8.5
(-3)
A 		14.7
(-1.2)
A 		8.6
(-3)
A 		21
avr 21.9
(3) 		19.6
(2) 		16.7
(0.7) 		9.2
(-2.6) 		19
(1.7) 		8.3
(-3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189577_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13478
Sequence Type Exemplar sequence
Representative Public ID F58B4.2 NCBI
Target Description F58B4.2 /REP_DB=WormBase Gene ID /WP=CE06012 /TR=Q20976 /GB=CAA98498.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome b5 like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073383
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073383 NCBI Caenorhabditis elegans F58B4.2 (F58B4.2) mRNA, complete cds. None
F58B4.2 ENSEMBL cdna:known chromosome:CEL140:V:10920294:10920988:-1 gene:F58B4.2 None
SNAP00000017992 ENSEMBL cdna:SNAP chromosome:CEL140:V:10920294:10920681:-1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10920293-10920988(-) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome b5 like
Ensembl F58B4.2
Entrez Gene 186494 Entrez gene
SwissProt Q20976 EMBL-EBI
WormBase CE35883 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA98498 Hypothetical protein F58B4.2 [Caenorhabditis elegans] ref|NP_505784.2| F58B4.2 [Caenorhabditis elegans] 2.0E-47
blast CAE64916 Hypothetical protein CBG09736 [Caenorhabditis briggsae] 2.0E-41
blast CAA98498 Hypothetical protein F58B4.2 [Caenorhabditis elegans] ref|NP_505784.2| F58B4.2 [Caenorhabditis elegans] 3.0E-24
blast CAE64916 Hypothetical protein CBG09736 [Caenorhabditis briggsae] 2.0E-21
blast BAA10888.1 Cytochrome b5 [Ascaris suum] 4.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR001199 EMBL-EBI Cytochrome b5 4.9E-22
Pfam IPR001199 EMBL-EBI Cytochrome b5 1.8E-9
Sequence
Target Sequence 
>C. ELEGANS:189577_AT
gtggaacaacattgcactcacgatgatctgtggcttttgttcagagacaaggtttataat
atgactccatatttcaaccaacaccctggaggattagcaattcttcgatatgcaggaaag
atcacacaaaacagtaaaacttggacattgtgtaaatgttatttcaacgttgtttcccaa
caattatttttccaggacgtaacaacagttctaccatttgttgtatctcatggcattgct
tggaaaatcgtgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGGAACAACATTGCACTCACGATG 510 491 61 Antisense
GCACTCACGATGATCTGTGGCTTTT 494 315 74 Antisense
GATCTGTGGCTTTTGTTCAGAGACA 291 419 85 Antisense
ATATTTCAACCAACACCCTGGAGGA 371 19 129 Antisense
ACCCTGGAGGATTAGCAATTCTTCG 548 89 143 Antisense
TATTTCAACGTTGTTTCCCAACAAT 653 653 220 Antisense
TCCCAACAATTATTTTTCCAGGACG 549 597 235 Antisense
TCCAGGACGTAACAACAGTTCTACC 421 231 251 Antisense
ACAGTTCTACCATTTGTTGTATCTC 597 107 265 Antisense
CATTTGTTGTATCTCATGGCATTGC 226 215 275 Antisense
CATGGCATTGCTTGGAAAATCGTGG 113 209 289 Antisense
Sequence Source Affymetrix Proprietary Database