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[189570_at] heat shock protein

Description F08H9.4 /REP_DB=WormBase Gene ID /WP=CE09268 /TR=Q19228 /GB=CAB01147.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=heat shock protein
Cluster 6grp_total > 78      4grp_total > 03      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 161
1 2 3 4 range
A 27.5
(-1.6)
A 		3.5
(-3)
A 		14.5
(-2.4)
A 		110
(3)
P 		107
B 30.3
(-2.1)
A 		14.6
(-2.7)
A 		6.4
(-3)
A 		163.7
(3)
P 		157
C 4.4
(-2.9)
A 		20.8
(-2.3)
A 		3
(-3)
A 		151.4
(3)
P 		148
avr 20.7
(-2.4) 		13
(-2.8) 		8
(-3) 		141.7
(3) 		134

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 302
1 2 3 4 5 6 range
A 50.4
(-3)
P 		72.7
(-2.2)
P 		76.6
(-2.1)
P 		59.3
(-2.7)
A 		158.3
(0.8)
P 		221.5
(3)
P 		171
B 77.1
(-2.1)
P 		75.7
(-2.1)
P 		49.1
(-2.8)
P 		42.1
(-3)
A 		170.6
(0.4)
P 		270.8
(3)
P 		229
C 52.6
(-2.4)
P 		47.8
(-2.5)
P 		24
(-3)
A 		50.1
(-2.5)
M 		113.4
(-1.2)
P 		326.1
(3)
P 		302
avr 60
(-2.7) 		65.4
(-2.6) 		49.9
(-3) 		50.5
(-3) 		147.4
(-0.4) 		272.8
(3) 		223
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 10
(0.6)
A 		0.8
(-3)
A 		3.8
(-1.8)
A 		16.1
(3)
P 		15
B 9.7
(-1.5)
A 		3.6
(-2.6)
A 		1.5
(-3)
A 		34.9
(3)
P 		33
C 1.5
(-2.9)
A 		6
(-1.8)
A 		1.1
(-3)
A 		25.5
(3)
P 		24
avr 7.1
(-1.7) 		3.5
(-2.6) 		2.1
(-3) 		25.5
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 9.3
(-3)
P 		25.8
(-0.8)
P 		16.7
(-2)
P 		15
(-2.2)
A 		47.8
(2.2)
P 		53.9
(3)
P 		45
B 26.8
(-2)
P 		25.2
(-2.1)
P 		11.9
(-2.9)
P 		11.1
(-3)
A 		69.3
(0.9)
P 		101
(3)
P 		90
C 19.5
(-2.2)
P 		14.6
(-2.5)
P 		6.1
(-3)
A 		10.1
(-2.8)
M 		36.7
(-1.2)
P 		109.2
(3)
P 		103
avr 18.5
(-2.5) 		21.9
(-2.2) 		11.6
(-3) 		12.1
(-3) 		51.3
(0.1) 		88
(3) 		76
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189570_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13452
Sequence Type Exemplar sequence
Representative Public ID F08H9.4 NCBI
Target Description F08H9.4 /REP_DB=WormBase Gene ID /WP=CE09268 /TR=Q19228 /GB=CAB01147.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=heat shock protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074185(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074185 NCBI Caenorhabditis elegans F08H9.4 (F08H9.4) mRNA, complete cds. 11/11 None
SNAP00000029729 ENSEMBL cdna:SNAP chromosome:CEL140:V:14466177:14466963:-1 11/11 None
GENEFINDER00000029736 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14466177:14469377:-1 11/11 None
F08H9.4 ENSEMBL cdna:known chromosome:CEL140:V:14466177:14466963:-1 gene:F08H9.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14466176-14466963(-) 100.0 100.0
Public Domain and Genome References
Gene Title heat shock protein
Ensembl F08H9.4
Entrez Gene 184215 Entrez gene
SwissProt Q19228 EMBL-EBI
WormBase CE09268 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01147 Hypothetical protein F08H9.4 [Caenorhabditis elegans] ref|NP_506586.1| F08H9.4 [Caenorhabditis elegans] 2.0E-80
blast CAB01147 Hypothetical protein F08H9.4 [Caenorhabditis elegans] ref|NP_506586.1| F08H9.4 [Caenorhabditis elegans] 2.0E-79
blast CAH60768 Hypothetical protein F08H9.12 [Caenorhabditis elegans] ref|NP_001023774.1| Serpentine Receptor, class Z family member (srz-97) [Caenorhabditis elegans] 2.0E-63
blast CAE72095 Hypothetical protein CBG19186 [Caenorhabditis briggsae] 3.0E-42
blast CAE72095 Hypothetical protein CBG19186 [Caenorhabditis briggsae] 3.0E-41
blast NP_503507 Heat Shock Protein family member (hsp-16.2) [Caenorhabditis elegans] gb|AAF60615.1| Heat shock protein protein 16.2 [Caenorhabditis elegans] sp|P06582|HSP12_CAEEL Heat shock protein Hsp-16.2 gb|AAA28071.1| heat shock protein 16-2 3.0E-39
Protein Domains
Database ID Description E-Value
Pfam IPR002068 EMBL-EBI Heat shock protein Hsp20 9.1E-28
Pfam IPR003845 EMBL-EBI Protein of unknown function DUF216 6.1E-5
Pfam IPR002068 EMBL-EBI Heat shock protein Hsp20 9.1E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000029736 6 12-34,38-60,73-95,110-132,152-171,191-213
Sequence
Target Sequence 
>C. ELEGANS:189570_AT
cttcaacccaaaacgagactctcaactcggtgaaatgatgagagatatgggcggaatgca
acgccggcttatgccaatttctggaacttttaacccaatgaccgatgattctgagataat
gaattccaatgataaatttgctgtaaatctcaatgtctccaacttcaagccagaagagct
gaaagtcaatctggaaggaagacaactgtcaattcaaggagaacatgacgtggagaatga
acatggtgcatcgagaaagtcgttctcaagaatgattcttcttccagaagatgttgatat
tacttcagttgcaactaatctttcgaatgatggaaaactttgtattgaagcaccgaaatt
ggaaggtgtttgtggaagatcagtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTCAACCCAAAACGAGACTCTCAA 684 217 30 Antisense
GAGACTCTCAACTCGGTGAAATGAT 280 393 44 Antisense
ATGGGCGGAATGCAACGCCGGCTTA 110 49 76 Antisense
AACGCCGGCTTATGCCAATTTCTGG 417 145 89 Antisense
GGAACTTTTAACCCAATGACCGATG 501 527 112 Antisense
AATCTCAATGTCTCCAACTTCAAGC 682 169 175 Antisense
GGTGCATCGAGAAAGTCGTTCTCAA 385 501 274 Antisense
AAGTCGTTCTCAAGAATGATTCTTC 577 157 286 Antisense
GATGTTGATATTACTTCAGTTGCAA 190 411 319 Antisense
TCAGTTGCAACTAATCTTTCGAATG 94 623 334 Antisense
GGAAGGTGTTTGTGGAAGATCAGTT 158 533 390 Antisense
Sequence Source Affymetrix Proprietary Database