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[189566_at] cytochrome P450

Description T10B9.4 /REP_DB=WormBase Gene ID /WP=CE13541 /TR=SW:Q27516 /GB=CAB09134.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_total > 58      4grp_total > 09      6grp_cgc > 27      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 322
1 2 3 4 range
A 18.1
(-3)
A 		77.2
(-1.9)
A 		80.2
(-1.8)
A 		340.3
(3)
P 		322
B 79.4
(-3)
A 		82.9
(-2.9)
A 		87.5
(-2.7)
A 		271.7
(3)
P 		192
C 63.5
(-2.9)
A 		58.3
(-3)
A 		58.8
(-3)
A 		312.3
(3)
P 		254
avr 53.7
(-3) 		72.8
(-2.5) 		75.5
(-2.5) 		308.1
(3) 		254

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 127
1 2 3 4 5 6 range
A 157.6
(2.5)
A 		71
(-1.9)
A 		57.6
(-2.6)
A 		56.9
(-2.6)
A 		49
(-3)
A 		167.7
(3)
P 		119
B 78.2
(-0.6)
A 		51.3
(-3)
A 		57.9
(-2.4)
A 		54.7
(-2.7)
A 		58.7
(-2.3)
A 		118.7
(3)
P 		67
C 94.7
(2)
A 		83.7
(1)
A 		67.6
(-0.5)
A 		88.1
(1.4)
A 		41
(-3)
A 		105.8
(3)
P 		65
avr 110.2
(1.5) 		68.7
(-1.6) 		61
(-2.2) 		66.6
(-1.7) 		49.6
(-3) 		130.7
(3) 		81
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 6.6
(-3)
A 		16.8
(-1.6)
A 		21
(-1)
A 		49.8
(3)
P 		43
B 25.3
(-2.2)
A 		20.5
(-3)
A 		20.3
(-3)
A 		57.9
(3)
P 		38
C 21
(-2.3)
A 		16.8
(-3)
A 		20.5
(-2.4)
A 		52.7
(3)
P 		36
avr 17.6
(-3) 		18
(-2.9) 		20.6
(-2.5) 		53.5
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 29.1
(0.5)
A 		25.2
(-0.3)
A 		12.5
(-3)
A 		14.4
(-2.6)
A 		14.8
(-2.5)
A 		40.8
(3)
P 		28
B 27.2
(-0.4)
A 		17.1
(-2.4)
A 		14
(-3)
A 		14.5
(-2.9)
A 		23.8
(-1.1)
A 		44.3
(3)
P 		30
C 35.1
(2.9)
A 		25.5
(0.3)
A 		17.2
(-1.9)
A 		17.7
(-1.8)
A 		13.3
(-3)
A 		35.4
(3)
P 		22
avr 30.5
(0.7) 		22.6
(-1.1) 		14.6
(-3) 		15.5
(-2.8) 		17.3
(-2.4) 		40.2
(3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189566_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3095
Sequence Type Exemplar sequence
Representative Public ID T10B9.4 NCBI
Target Description T10B9.4 /REP_DB=WormBase Gene ID /WP=CE13541 /TR=SW:Q27516 /GB=CAB09134.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063714(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063714 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-13A8) (cyp-13A8) mRNA, complete cds. 11/11 None
SNAP00000024371 ENSEMBL cdna:SNAP chromosome:CEL140:II:9805538:9806657:-1 11/11 None
T10B9.4 ENSEMBL cdna:known chromosome:CEL140:II:9805538:9807443:-1 gene:T10B9.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9805534-9807440(-) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-13A8
Ensembl T10B9.4
Entrez Gene 188358 Entrez gene
SwissProt Q27516 EMBL-EBI
WormBase CE13541 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:246978_AT cytochrome P450 family protein at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88611 Hypothetical protein T10B9.4 [Caenorhabditis elegans] emb|CAB09134.1| Hypothetical protein T10B9.4 [Caenorhabditis elegans] ref|NP_496115.1| CYtochrome P450 family member (cyp-13A8) [Caenorhabditis elegans] sp|Q27516|C13A8_CAEEL Putative cytochrome P450 CYP13A8 0.0
blast CAE57967 Hypothetical protein CBG01028 [Caenorhabditis briggsae] 0.0
blast CAA88611 Hypothetical protein T10B9.4 [Caenorhabditis elegans] emb|CAB09134.1| Hypothetical protein T10B9.4 [Caenorhabditis elegans] ref|NP_496115.1| CYtochrome P450 family member (cyp-13A8) [Caenorhabditis elegans] sp|Q27516|C13A8_CAEEL Putative cytochrome P450 CYP13A8 1.0E-179
blast CAA88610 Hypothetical protein T10B9.8 [Caenorhabditis elegans] ref|NP_496108.1| CYtochrome P450 family member (cyp-13A1) [Caenorhabditis elegans] sp|Q27520|C13A1_CAEEL Putative cytochrome P450 CYP13A1 1.0E-170
blast CAE57967 Hypothetical protein CBG01028 [Caenorhabditis briggsae] 1.0E-129
blast CAA88610 Hypothetical protein T10B9.8 [Caenorhabditis elegans] ref|NP_496108.1| CYtochrome P450 family member (cyp-13A1) [Caenorhabditis elegans] sp|Q27520|C13A1_CAEEL Putative cytochrome P450 CYP13A1 2.0E-99
Protein Families
Method ID Description E-Value
p450 CP14_CHICK Animalia; CYP1A4; chi; CP14_CHICK; Gallus gallus; P79760 2.0E-73
p450 CP14_CHICK Animalia; CYP1A4; chi; CP14_CHICK; Gallus gallus; P79760 1.0E-126
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.6E-20
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.0E-57
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.0E-57
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000024371 2 6-28,114-136
Sequence
Target Sequence 
>C. ELEGANS:189566_AT
ataaccgacaactgaccactcaagaaatcattagccagtgctttgtatttttagttgctg
gatttgatactacagccatttccctttcctatgttacatacttcttggcattgaatccaa
aaattcagagcaagttacaagacgaagtagacaaagaatgtccaaatgatgagatcacat
ttgatcagctttcaaaattgaaatatatggataatgtgatcaaggagtcattaagattgt
tcccatttgcttcttttgcaaactcccgccgatgcatgagaaatacggttatcggagagc
aaattgtagaagctggtgttgatgtgatgatcgacacctggactcttcaccatgacaaaa
atgtatggggaaatgacgtagaagagttcaagcctgaaagatgggactctccattaactc
ctcaacaagcatacctctcatttggtgctggacccagagtatgtctcgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATAACCGACAACTGACCACTCAAGA 627 21 914 Antisense
GATTTGATACTACAGCCATTTCCCT 196 427 974 Antisense
CATTTCCCTTTCCTATGTTACATAC 323 215 990 Antisense
TAAGATTGTTCCCATTTGCTTCTTT 624 637 1145 Antisense
AAACTCCCGCCGATGCATGAGAAAT 524 127 1173 Antisense
GATGTGATGATCGACACCTGGACTC 638 407 1234 Antisense
ACCTGGACTCTTCACCATGACAAAA 263 89 1249 Antisense
AGATGGGACTCTCCATTAACTCCTC 68 69 1312 Antisense
TTAACTCCTCAACAAGCATACCTCT 93 683 1327 Antisense
CAAGCATACCTCTCATTTGGTGCTG 228 185 1339 Antisense
GTGCTGGACCCAGAGTATGTCTCGG 218 479 1358 Antisense
Sequence Source Affymetrix Proprietary Database