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[189559_s_at] Helix-turn-helix

Description H21P03.1 /REP_DB=WormBase Gene ID /WP=CE18819 /TR=Q9XTV4 /GB=CAB09112.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Helix-turn-helix
Cluster 6grp_highest_10_10 > 72      6grp_high1_10_10 > 37      4grp_highest_8_8 > 11      4grp_high_8_8 > 39      6grp_highest_nonorm > 67      6grp_total > 17      4grp_total > 40      6grp_highest_sub > Cu      6grp_cgc > 06      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1337
1 2 3 4 range
A 1445.6
(-3)
P 		2067.5
(0.4)
P 		2558.9
(3)
P 		2134.1
(0.7)
P 		1113
B 1474.6
(-3)
P 		1796.2
(-1.5)
P 		2782.4
(3)
P 		1971.6
(-0.7)
P 		1308
C 1591.6
(-3)
P 		2005.4
(0.6)
P 		2280.4
(3)
P 		1737.6
(-1.7)
P 		689
avr 1503.9
(-3) 		1956.4
(-0.4) 		2540.6
(3) 		1947.8
(-0.4) 		1037

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1144
1 2 3 4 5 6 range
A 2273
(3)
P 		1541.8
(-0.8)
P 		1623.3
(-0.4)
P 		1128.8
(-3)
P 		1638
(-0.3)
P 		1368.4
(-1.7)
P 		1144
B 1961.1
(2.8)
P 		1561.1
(-3)
P 		1974.3
(3)
P 		1648.1
(-1.7)
P 		1602.2
(-2.4)
P 		1627.8
(-2)
P 		413
C 1506.7
(0.1)
P 		1559.6
(0.8)
P 		1402.8
(-1.3)
P 		1521.9
(0.3)
P 		1272.3
(-3)
P 		1724.5
(3)
P 		452
avr 1913.6
(3) 		1554.2
(-1.5) 		1666.8
(-0.1) 		1432.9
(-3) 		1504.2
(-2.1) 		1573.6
(-1.2) 		481
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 502
1 2 3 4 range
A 526.3
(0.6)
P 		448.8
(-0.7)
P 		669
(3)
P 		312.4
(-3)
P 		357
B 470.2
(-1.7)
P 		443.6
(-2.4)
P 		644.7
(3)
P 		420.1
(-3)
P 		225
C 526.8
(-0.2)
P 		576.6
(0.4)
P 		794.8
(3)
P 		293
(-3)
P 		502
avr 507.8
(-0.2) 		489.7
(-0.5) 		702.8
(3) 		341.8
(-3) 		361

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 396
1 2 3 4 5 6 range
A 419
(0)
P 		548.3
(3)
P 		353.5
(-1.5)
P 		286.3
(-3)
P 		494.3
(1.8)
P 		333.2
(-1.9)
P 		262
B 682.4
(3)
P 		519.6
(-1)
P 		479.4
(-2)
P 		436.5
(-3)
P 		650.9
(2.2)
P 		607
(1.2)
P 		246
C 558.5
(2.6)
P 		475.9
(0.8)
P 		357
(-1.9)
P 		306
(-3)
P 		411.7
(-0.7)
P 		577.3
(3)
P 		271
avr 553.3
(3) 		514.6
(1.9) 		396.6
(-1.5) 		342.9
(-3) 		519
(2) 		505.8
(1.6) 		210
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189559_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9603
Sequence Type Exemplar sequence
Representative Public ID H21P03.1 NCBI
Target Description H21P03.1 /REP_DB=WormBase Gene ID /WP=CE18819 /TR=Q9XTV4 /GB=CAB09112.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Helix-turn-helix
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069765(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069765 NCBI Caenorhabditis elegans H21P03.1 (Helix-turn-helix) mRNA, complete cds. 11/11 None
SNAP00000044458 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11479606:11480123:-1 11/11 None
GENEFINDER00000044463 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11479606:11480123:-1 11/11 None
H21P03.1 ENSEMBL cdna:known chromosome:CEL140:IV:11479341:11480156:-1 gene:H21P03.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11479611-11480129(-) 100.0 100.0
Public Domain and Genome References
Gene Title Helix-turn-helix
Gene Symbol mbf-1
Ensembl H21P03.1
Entrez Gene 178071 Entrez gene
SwissProt Q9XTV4 EMBL-EBI
WormBase CE18819 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251551_AT ethylene-responsive transcriptional coactivator, putative at
CANINE:1588345_AT similar to endothelial differentiation-related factor 1 isoform alpha cfa
CANINE_2:CFA.15077.1.S1_AT similar to endothelial differentiation-related factor 1 isoform alpha cfa
CANINE_2:CFAAFFX.29880.1.S1_S_AT similar to endothelial differentiation-related factor 1 isoform alpha cfa
DROSOPHILA_2:1634722_S_AT multiprotein bridging factor 1 dm
DROSGENOME1:152287_AT multiprotein bridging factor 1 dm
CHICKEN:GGAAFFX.11567.1.S1_AT endothelial differentiation-related factor 1 gga
CHICKEN:GGAAFFX.11567.1.S1_S_AT endothelial differentiation-related factor 1 gga
HG-U133A:209059_S_AT endothelial differentiation-related factor 1 hs
HG-U133A_2:209059_S_AT endothelial differentiation-related factor 1 hs
HG-U133_PLUS_2:209059_S_AT endothelial differentiation-related factor 1 hs
HG-U133_PLUS_2:209058_AT endothelial differentiation-related factor 1 hs
HG-U133A_2:209058_AT endothelial differentiation-related factor 1 hs
HG-U133A:209058_AT endothelial differentiation-related factor 1 hs
HG-FOCUS:209058_AT endothelial differentiation-related factor 1 hs
HU35KSUBA:RC_AA598725_AT endothelial differentiation-related factor 1 hs
U133_X3P:G6526354_3P_A_AT endothelial differentiation-related factor 1 hs
MG-U74AV2:96322_AT endothelial differentiation-related factor 1 mm
MOE430A:1438503_X_AT endothelial differentiation-related factor 1 mm
MOUSE430A_2:1438503_X_AT endothelial differentiation-related factor 1 mm
MOUSE430_2:1438503_X_AT endothelial differentiation-related factor 1 mm
MOE430A:1448266_AT endothelial differentiation-related factor 1 mm
MOUSE430A_2:1448266_AT endothelial differentiation-related factor 1 mm
MOUSE430_2:1448266_AT endothelial differentiation-related factor 1 mm
MOE430B:1440151_S_AT endothelial differentiation-related factor 1 mm
MOUSE430_2:1440151_S_AT endothelial differentiation-related factor 1 mm
MOE430B:1440152_X_AT endothelial differentiation-related factor 1 mm
MOUSE430_2:1440152_X_AT endothelial differentiation-related factor 1 mm
MU19KSUBB:TC34473_AT Endothelial differentiation-related factor 1, mRNA (cDNA clone MGC:30996 IMAGE:5250117) mm
RG-U34A:RC_AA891746_AT endothelial differentiation-related factor 1 (predicted) rn
RAE230A:1375189_AT endothelial differentiation-related factor 1 (predicted) rn
RAT230_2:1375189_AT endothelial differentiation-related factor 1 (predicted) rn
YEAST_2:1771596_AT Transcriptional coactivator that bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB09112 Hypothetical protein H21P03.1 [Caenorhabditis elegans] ref|NP_502166.1| H21P03.1 [Caenorhabditis elegans] 3.0E-84
blast CAE62111 Hypothetical protein CBG06149 [Caenorhabditis briggsae] 2.0E-76
blast XP_537793 PREDICTED: similar to endothelial differentiation-related factor 1 isoform alpha [Canis familiaris] 3.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR001387 EMBL-EBI Helix-turn-helix motif 5.8E-12
Sequence
Target Sequence 
>C. ELEGANS:189559_S_AT
atgtccaagtatggctgcccaacctctgacactgatccaaactcggtcacaatgattacg
aagcgaggaccagtgaacaagacattgaaatcggctgctcaattgaatgctgctcagcgt
gctggagttgatatttcgaccgaaaagaagacgatgtctggtggcaatcgccagcacagc
gccaacaagaacactctgcggcttgatgaagaaacggaggagcttcatcaccagaaagtt
gctctgtccctcggaaaagtcatgcagcaggctcgtgccacgaaaggttggacgcagaaa
gatctttcaacgcaaattaatgagaagccgcaagtagtcggcgaatacgaaagcggaaag
gctgtcccaaatcaacagatcatggcaaaaatggagcgtgcgcttggagtcaagttgcgc
ggcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTCCAAGTATGGCTGCCCAACCT 524 45 13 Antisense
ACACTGATCCAAACTCGGTCACAAT 675 109 41 Antisense
AATGCTGCTCAGCGTGCTGGAGTTG 214 165 118 Antisense
GTCTGGTGGCAATCGCCAGCACAGC 477 471 168 Antisense
GCGCCAACAAGAACACTCTGCGGCT 252 287 191 Antisense
AAACGGAGGAGCTTCATCACCAGAA 602 125 224 Antisense
CACCAGAAAGTTGCTCTGTCCCTCG 185 201 241 Antisense
AAAAGTCATGCAGCAGGCTCGTGCC 294 133 267 Antisense
GCTCGTGCCACGAAAGGTTGGACGC 537 299 283 Antisense
GCGGAAAGGCTGTCCCAAATCAACA 638 293 365 Antisense
GCTTGGAGTCAAGTTGCGCGGCAAA 439 307 414 Antisense
Sequence Source Affymetrix Proprietary Database