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[189557_at]

Description B0228.5 /REP_DB=WormBase Gene ID /WP=CE01745 /TR=SW:Q09433 /GB=AAC38808.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Thioredoxin I
Cluster 6grp_total > 58      4grp_total > 13      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 100
1 2 3 4 range
A 62.5
(0.2)
P 		51.2
(-0.6)
P 		15.2
(-3)
A 		103.9
(3)
P 		89
B 37.5
(0)
A 		28.1
(-1.2)
A 		14.6
(-3)
A 		60.1
(3)
A 		46
C 53.7
(0.5)
P 		4.4
(-3)
A 		17.7
(-2.1)
A 		88.5
(3)
P 		84
avr 51.2
(0.1) 		27.9
(-1.9) 		15.8
(-3) 		84.2
(3) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 47.5
(1)
A 		20.6
(-2.8)
A 		19.1
(-3)
A 		24.6
(-2.2)
A 		27.4
(-1.8)
A 		61.9
(3)
P 		43
B 39.8
(0.4)
A 		32.3
(-0.8)
A 		37.9
(0.1)
A 		18.5
(-3)
A 		55.7
(3)
P 		37.1
(0)
P 		37
C 46.4
(-0.1)
M 		41.7
(-0.4)
A 		11.6
(-3)
A 		45
(-0.2)
M 		13.7
(-2.8)
A 		82.4
(3)
P 		71
avr 44.6
(0.5) 		31.5
(-1.6) 		22.9
(-3) 		29.4
(-2) 		32.3
(-1.5) 		60.5
(3) 		38
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 22.8
(3)
P 		11.1
(-0.7)
P 		4
(-3)
A 		15.2
(0.6)
P 		19
B 11.9
(2.4)
A 		6.9
(-0.8)
A 		3.4
(-3)
A 		12.8
(3)
A 		9
C 17.8
(3)
P 		1.3
(-3)
A 		6.2
(-1.2)
A 		14.9
(1.9)
P 		17
avr 17.5
(3) 		6.4
(-2.1) 		4.5
(-3) 		14.3
(1.5) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 8.8
(-0.5)
A 		7.3
(-1.3)
A 		4.2
(-3)
A 		6.2
(-1.9)
A 		8.3
(-0.7)
A 		15.1
(3)
P 		11
B 13.8
(0)
A 		10.8
(-1)
A 		9.2
(-1.5)
A 		4.9
(-3)
A 		22.6
(3)
P 		13.8
(0)
P 		18
C 17.2
(0.5)
M 		12.7
(-0.6)
A 		2.9
(-3)
A 		9.1
(-1.5)
M 		4.4
(-2.6)
A 		27.6
(3)
P 		25
avr 13.3
(0.5) 		10.3
(-0.8) 		5.4
(-3) 		6.7
(-2.4) 		11.8
(-0.1) 		18.8
(3) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189557_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4750
Sequence Type Exemplar sequence
Representative Public ID B0228.5 NCBI
Target Description B0228.5 /REP_DB=WormBase Gene ID /WP=CE01745 /TR=SW:Q09433 /GB=AAC38808.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Thioredoxin I
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026715(11), NM_001026714(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026715 NCBI Caenorhabditis elegans B0228.5b (B0228.5) mRNA, complete cds. 11/11 None
NM_001026714 NCBI Caenorhabditis elegans B0228.5a (B0228.5) mRNA, complete cds. 11/11 None
SNAP00000020603 ENSEMBL cdna:SNAP chromosome:CEL140:II:7752737:7753459:-1 11/11 None
GENEFINDER00000020740 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7752737:7753459:-1 11/11 None
B0228.5b ENSEMBL cdna:known chromosome:CEL140:II:7752737:7753459:-1 gene:B0228.5 11/11 None
B0228.5a ENSEMBL cdna:known chromosome:CEL140:II:7752737:7753409:-1 gene:B0228.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7752733-7753406(-) 100.0 100.0
Public Domain and Genome References
Ensembl B0228.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC38808 Hypothetical protein B0228.5a [Caenorhabditis elegans] ref|NP_001021885.1| B0228.5a [Caenorhabditis elegans] sp|Q09433|THIO1_CAEEL Probable thioredoxin B0228.5 3.0E-63
blast AAP86623 Hypothetical protein B0228.5b [Caenorhabditis elegans] ref|NP_001021886.1| B0228.5b [Caenorhabditis elegans] 2.0E-61
blast AAC38808 Hypothetical protein B0228.5a [Caenorhabditis elegans] ref|NP_001021885.1| B0228.5a [Caenorhabditis elegans] sp|Q09433|THIO1_CAEEL Probable thioredoxin B0228.5 1.0E-53
blast AAP86623 Hypothetical protein B0228.5b [Caenorhabditis elegans] ref|NP_001021886.1| B0228.5b [Caenorhabditis elegans] 1.0E-53
Protein Domains
Database ID Description E-Value
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 5.0E-42
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 1.3E-43
Sequence
Target Sequence 
>C. ELEGANS:189557_AT
gagcaattgatccgtcaacatccggagaagatcattattcttgatttctatgcaacttgg
tgcggaccatgcaaagcaattgcaccattatacaaagaattagctacaactcacaaagga
atcatcttctgcaaagttgatgtcgatgaagcggaagatctttgttccaaatatgatgtc
aagatgatgccgactttcattttcaccaagaatggagacgcaattgaggcactggaaggc
tgcgttgaggacgaactgcgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCAATTGATCCGTCAACATCCGG 150 391 67 Antisense
GATTTCTATGCAACTTGGTGCGGAC 199 429 109 Antisense
GGTGCGGACCATGCAAAGCAATTGC 356 501 125 Antisense
GAATTAGCTACAACTCACAAAGGAA 658 321 163 Antisense
AAGGAATCATCTTCTGCAAAGTTGA 450 161 182 Antisense
GAAGCGGAAGATCTTTGTTCCAAAT 32 343 214 Antisense
GATGTCAAGATGATGCCGACTTTCA 578 409 241 Antisense
GCCGACTTTCATTTTCACCAAGAAT 571 281 255 Antisense
AATGGAGACGCAATTGAGGCACTGG 288 163 277 Antisense
GAGGCACTGGAAGGCTGCGTTGAGG 206 403 292 Antisense
GGCTGCGTTGAGGACGAACTGCGTC 447 539 304 Antisense
Sequence Source Affymetrix Proprietary Database