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[189551_s_at] Actins

Description T25C8.2 /REP_DB=WormBase Gene ID /WP=CE16463 /GEN=act-5 /TR=O45815 /GB=CAB05817.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Actins
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 04      4grp_highest_8_8 > 25      4grp_high_8_8 > 60      6grp_highest_nonorm > 15      6grp_total > 24      4grp_total > 60      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13259
1 2 3 4 range
A 11906.2
(1.4)
P 		14883.7
(2.7)
P 		15523.5
(3)
P 		2264.8
(-3)
P 		13259
B 13627.1
(2.3)
P 		13571.8
(2.3)
P 		15109.4
(3)
P 		2681.2
(-3)
P 		12428
C 12107.5
(2.2)
P 		13430
(2.9)
P 		13539
(3)
P 		2503.4
(-3)
P 		11036
avr 12546.9
(1.9) 		13961.8
(2.6) 		14724
(3) 		2483.1
(-3) 		12241

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10153
1 2 3 4 5 6 range
A 6075
(0.1)
P 		9174.4
(3)
P 		4641.9
(-1.2)
P 		4583.6
(-1.2)
P 		2655.2
(-3)
P 		3165.8
(-2.5)
P 		6519
B 5855.8
(2.6)
P 		6120.5
(3)
P 		4839.7
(1.3)
P 		3042.8
(-1.1)
P 		2817.4
(-1.4)
P 		1652.3
(-3)
P 		4468
C 11579.8
(3)
P 		6005.3
(-0.3)
P 		7637.2
(0.7)
P 		1866.8
(-2.7)
P 		4883.9
(-1)
P 		1427
(-3)
P 		10153
avr 7836.9
(3) 		7100.1
(2.2) 		5706.3
(0.8) 		3164.4
(-1.9) 		3452.2
(-1.6) 		2081.7
(-3) 		5755
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4387
1 2 3 4 range
A 4335.1
(3)
P 		3230.9
(1.3)
P 		4058.1
(2.6)
P 		331.5
(-3)
P 		4004
B 4345.6
(3)
P 		3351.9
(1.4)
P 		3501.2
(1.7)
P 		571.4
(-3)
P 		3774
C 4007.9
(2)
P 		3861.2
(1.8)
P 		4718.8
(3)
P 		422.1
(-3)
P 		4297
avr 4229.5
(3) 		3481.3
(1.8) 		4092.7
(2.8) 		441.7
(-3) 		3788

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3917
1 2 3 4 5 6 range
A 1119.9
(-2.2)
P 		3262.9
(3)
P 		1010.9
(-2.4)
P 		1162.5
(-2.1)
P 		801.2
(-2.9)
P 		770.8
(-3)
P 		2492
B 2037.5
(3)
P 		2037.2
(3)
P 		1175.2
(-0.6)
P 		805.9
(-2.2)
P 		1144.7
(-0.8)
P 		616.1
(-3)
P 		1421
C 4292.1
(3)
P 		1832.4
(-0.8)
P 		1943.8
(-0.6)
P 		375.4
(-3)
P 		1580.4
(-1.2)
P 		477.7
(-2.8)
P 		3917
avr 2483.2
(3) 		2377.5
(2.7) 		1376.6
(-0.6) 		781.3
(-2.5) 		1175.4
(-1.2) 		621.5
(-3) 		1862
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189551_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7697
Sequence Type Exemplar sequence
Representative Public ID T25C8.2 NCBI
Target Description T25C8.2 /REP_DB=WormBase Gene ID /WP=CE16463 /GEN=act-5 /TR=O45815 /GB=CAB05817.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Actins
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067408(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067408 NCBI Caenorhabditis elegans T25C8.2 (Actins) mRNA, complete cds. 11/11 None
SNAP00000008506 ENSEMBL cdna:SNAP chromosome:CEL140:III:13604070:13605582:-1 11/11 None
GENEFINDER00000008513 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13604070:13605582:-1 11/11 None
T25C8.2.1 ENSEMBL cdna:known chromosome:CEL140:III:13603855:13605582:-1 gene:T25C8.2 11/11 None
T25C8.2.3 ENSEMBL cdna:known chromosome:CEL140:III:13603855:13605583:-1 gene:T25C8.2 11/11 None
T25C8.2.2 ENSEMBL cdna:known chromosome:CEL140:III:13603855:13605584:-1 gene:T25C8.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13604065-13605578(-) 100.0 100.0
Public Domain and Genome References
Gene Title Actins
Gene Symbol act-5
Ensembl T25C8.2
Entrez Gene 176793 Entrez gene
SwissProt O45815 EMBL-EBI
WormBase CE16463 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:260765_AT actin 8 (ACT8) at
ATGENOME1:15945_AT actin 8 (ACT8) at
CANINE:1604121_X_AT hypothetical LOC475923 cfa
CANINE_2:CFA.9114.2.S1_S_AT hypothetical LOC475923 cfa
CANINE_2:CFAAFFX.9553.1.S1_S_AT hypothetical LOC475923 cfa
DROSOPHILA_2:1636348_S_AT Actin 87E dm
DROSGENOME1:149844_F_AT Actin 87E dm
HG-U95AV2:31737_AT actin, alpha 1, skeletal muscle hs
HG-FOCUS:203872_AT actin, alpha 1, skeletal muscle hs
HG-U133A_2:203872_AT actin, alpha 1, skeletal muscle hs
HG-U133A:203872_AT actin, alpha 1, skeletal muscle hs
HG-U133_PLUS_2:203872_AT actin, alpha 1, skeletal muscle hs
HUGENEFL:M20543_AT actin, alpha 1, skeletal muscle hs
U133_X3P:G5016087_3P_AT actin, alpha 1, skeletal muscle hs
HG-U95D:73283_F_AT actin, alpha 1, skeletal muscle hs
MOE430A:1427735_A_AT actin, alpha 1, skeletal muscle mm
MOUSE430A_2:1427735_A_AT actin, alpha 1, skeletal muscle mm
MOUSE430_2:1427735_A_AT actin, alpha 1, skeletal muscle mm
MU11KSUBA:M12347_S_AT actin, alpha 1, skeletal muscle mm
MU11KSUBB:MSA.8919.0_F_AT actin, alpha 1, skeletal muscle mm
MG-U74AV2:100381_AT actin, alpha 1, skeletal muscle mm
MU19KSUBA:TC18546_AT Actin, alpha 1, skeletal muscle, mRNA (cDNA clone MGC:18923 IMAGE:3587657) mm
MU19KSUBB:TC32489_AT Actin, alpha 1, skeletal muscle, mRNA (cDNA clone MGC:18923 IMAGE:3587657) mm
RG-U34A:J00692_AT actin, alpha 1, skeletal muscle rn
RAE230A:1369928_AT actin, alpha 1, skeletal muscle rn
RAT230_2:1369928_AT actin, alpha 1, skeletal muscle rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5884 actin filament inferred from electronic annotation QuickGO AmiGO
15629 actin cytoskeleton inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3774 motor activity inferred from electronic annotation QuickGO AmiGO
5200 structural constituent of cytoskeleton inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05817 Hypothetical protein T25C8.2 [Caenorhabditis elegans] ref|NP_499809.1| T25C8.2 [Caenorhabditis elegans] 0.0
blast CAE71353 Hypothetical protein CBG18256 [Caenorhabditis briggsae] 0.0
blast CAA74015 actin [Saccoglossus kowalevskii] sp|O18499|ACT1_SACKO Actin-1 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004000 EMBL-EBI Actin/actin-like 1.0E-126
Pfam IPR004000 EMBL-EBI Actin/actin-like 8.8E-124
Pfam IPR004000 EMBL-EBI Actin/actin-like 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:189551_S_AT
acgcgacttgaccgactacatgatgaagatcctcaccgagcgcggttacacttttaccac
caccgccgaacgcgaaatcgtccgggacatcaaagagaagctttgctacgtcgcccacga
cttcgagtccgagctcgccgccgccgcctcctcctcctccctcgagaagagctacgagct
tcccgacggacaggttatcaccatcggaaacgagagattccgctgcccagaagtcctctt
ccagcctgccttcatcggaatggaaggtgccggaatccacgagaccacctaccaatccat
catgaagtgcgatgtcgacatcagaaaggatctctacgccaacactgttctctccggagg
tacctccatgttcccaggaatcgctgatcgtatgcaaaaggagattcaacatttggctcc
aagcacaatgaagatcaagattattgctccaccagagcgcaagtactccgtctggatcgg
aggatctattcttgcttccctctcaaccttccagcaaatgtgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGCGACTTGACCGACTACATGATG 503 91 558 Antisense
ACATGATGAAGATCCTCACCGAGCG 478 105 575 Antisense
GCCGAACGCGAAATCGTCCGGGACA 272 285 622 Antisense
TACGAGCTTCCCGACGGACAGGTTA 637 647 730 Antisense
GGACAGGTTATCACCATCGGAAACG 18 527 745 Antisense
ACCACCTACCAATCCATCATGAAGT 505 85 841 Antisense
TCAGAAAGGATCTCTACGCCAACAC 279 625 878 Antisense
TCTCCGGAGGTACCTCCATGTTCCC 180 611 908 Antisense
ATGTTCCCAGGAATCGCTGATCGTA 357 47 925 Antisense
GATTATTGCTCCACCAGAGCGCAAG 702 429 996 Antisense
CCTCTCAACCTTCCAGCAAATGTGG 30 269 1056 Antisense
Sequence Source Affymetrix Proprietary Database