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[189550_at] helix-loop-helix

Description ZK682.4 /REP_DB=WormBase Gene ID /WP=CE05102 /TR=Q23579 /GB=AAA82418.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=helix-loop-helix
Cluster 6grp_total > 72      4grp_total > 47      6grp_cgc > 84      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 81
1 2 3 4 range
A 67.2
(3)
A 		52
(1.5)
A 		8.3
(-3)
A 		36.9
(-0.1)
A 		59
B 49.5
(3)
A 		13.1
(-2.1)
A 		6.4
(-3)
A 		38.3
(1.4)
A 		43
C 87.1
(3)
A 		12.9
(-3)
A 		50.4
(0)
A 		12.5
(-3)
A 		75
avr 67.9
(3) 		26
(-2.4) 		21.7
(-3) 		29.2
(-2) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 11
(-2.4)
A 		7.9
(-2.8)
A 		50.4
(2.3)
A 		40.1
(1.1)
A 		55.8
(3)
A 		6.2
(-3)
A 		50
B 47.5
(2.6)
A 		45.3
(2.3)
P 		50.1
(3)
A 		12.2
(-2.2)
A 		19.4
(-1.2)
A 		6.5
(-3)
A 		44
C 49.2
(-1.6)
A 		56
(-1.1)
A 		37.9
(-2.5)
A 		106.7
(3)
A 		64.6
(-0.4)
A 		31.8
(-3)
A 		75
avr 35.9
(0.3) 		36.4
(0.4) 		46.1
(1.9) 		53
(3) 		46.6
(2) 		14.8
(-3) 		38
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 24.5
(3)
A 		11.3
(-0.6)
A 		2.2
(-3)
A 		5.4
(-2.1)
A 		22
B 15.8
(3)
A 		3.2
(-2.3)
A 		1.5
(-3)
A 		8.2
(-0.2)
A 		14
C 28.8
(3)
A 		3.7
(-2.6)
A 		17.6
(0.5)
A 		2.1
(-3)
A 		27
avr 23
(3) 		6.1
(-2.7) 		7.1
(-2.4) 		5.2
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 2
(-2.8)
A 		2.8
(-2.5)
A 		11
(0.7)
A 		10.2
(0.4)
A 		16.8
(3)
A 		1.5
(-3)
A 		15
B 16.5
(3)
A 		15.1
(2.4)
P 		12.2
(1.2)
A 		3.2
(-2.7)
A 		7.9
(-0.7)
A 		2.4
(-3)
A 		14
C 18.2
(1.3)
A 		17.1
(0.8)
A 		9.6
(-3)
A 		21.5
(3)
A 		20.9
(2.7)
A 		10.6
(-2.5)
A 		12
avr 12.2
(1.3) 		11.7
(1) 		10.9
(0.5) 		11.6
(0.9) 		15.2
(3) 		4.8
(-3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189550_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12033
Sequence Type Exemplar sequence
Representative Public ID ZK682.4 NCBI
Target Description ZK682.4 /REP_DB=WormBase Gene ID /WP=CE05102 /TR=Q23579 /GB=AAA82418.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=helix-loop-helix
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073000(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073000 NCBI Caenorhabditis elegans ZK682.4 (helix-loop-helix) mRNA, complete cds. 11/11 None
SNAP00000025482 ENSEMBL cdna:SNAP chromosome:CEL140:V:9283287:9284636:-1 11/11 None
GENEFINDER00000025487 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9283287:9284621:-1 11/11 None
ZK682.4 ENSEMBL cdna:known chromosome:CEL140:V:9283287:9284021:-1 gene:ZK682.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9283286-9284021(-) 100.0 100.0
Public Domain and Genome References
Gene Title helix-loop-helix
Gene Symbol hlh-10
Ensembl ZK682.4
Entrez Gene 191402 Entrez gene
SwissProt Q23579 EMBL-EBI
WormBase CE05102 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA82418 Helix loop helix protein 10 [Caenorhabditis elegans] ref|NP_505401.1| ZK682.4 [Caenorhabditis elegans] 9.0E-84
blast AAA82418 Helix loop helix protein 10 [Caenorhabditis elegans] ref|NP_505401.1| ZK682.4 [Caenorhabditis elegans] 1.0E-83
blast CAE72146 Hypothetical protein CBG19244 [Caenorhabditis briggsae] 3.0E-39
blast CAE72146 Hypothetical protein CBG19244 [Caenorhabditis briggsae] 3.0E-38
blast CAE72146 Hypothetical protein CBG19244 [Caenorhabditis briggsae] 7.0E-33
Protein Domains
Database ID Description E-Value
Pfam IPR001092 EMBL-EBI Basic helix-loop-helix dimerization domain bHLH 2.2E-16
Pfam IPR001092 EMBL-EBI Basic helix-loop-helix dimerization domain bHLH 2.2E-16
Pfam IPR001092 EMBL-EBI Basic helix-loop-helix dimerization domain bHLH 2.2E-16
Sequence
Target Sequence 
>C. ELEGANS:189550_AT
tttacaacagaacctttttgcttggtttcttcagtccatgcttgaagcatctgcatctca
acctcaattaacacaagatgagccaccagaaaatgatacaaaggaaaatgacctcgttaa
gcaaaacaaatcagaagttaatgacgaaaatgagtctactccaagtccaactcaaaatag
caggcgaagaacttccactgggaagattgatagaagaatggtcggaaaaatgtgtacgag
aagatatgaagccaatgcaagagaaagaaatagagttcaacaactttcaaaaatgtttga
tcaattgagagtctgccttccaatagaggatgacgcaaaaatatcaaagttggctacgtt
gaaggttgcaagttcttacattggatatctcggagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTACAACAGAACCTTTTTGCTTGG 96 615 42 Antisense
TTGCTTGGTTTCTTCAGTCCATGCT 554 703 59 Antisense
CATGCTTGAAGCATCTGCATCTCAA 496 209 78 Antisense
TCTGCATCTCAACCTCAATTAACAC 218 609 91 Antisense
ATTAACACAAGATGAGCCACCAGAA 354 13 108 Antisense
GAGTCTACTCCAAGTCCAACTCAAA 709 399 193 Antisense
AATAGCAGGCGAAGAACTTCCACTG 525 171 217 Antisense
GATCAATTGAGAGTCTGCCTTCCAA 68 423 340 Antisense
GTCTGCCTTCCAATAGAGGATGACG 586 469 352 Antisense
GTTGGCTACGTTGAAGGTTGCAAGT 336 433 390 Antisense
GTTCTTACATTGGATATCTCGGAGC 458 443 413 Antisense
Sequence Source Affymetrix Proprietary Database