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[189549_at]

Description T06A4.1 /REP_DB=WormBase Gene ID /WP=CE18210 /TR=Q9TZH1 /GB=AAC67473.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=carboxypeptidase
Cluster 6grp_total > 10      4grp_total > 50      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 446
1 2 3 4 range
A 52.6
(-3)
A 		463.9
(3)
P 		295.8
(0.5)
P 		59.7
(-2.9)
A 		411
B 45.3
(-3)
A 		385.7
(3)
P 		286.5
(1.3)
P 		92.2
(-2.2)
A 		340
C 26.3
(-2.9)
A 		361.9
(3)
P 		259.9
(1.2)
P 		18.1
(-3)
A 		344
avr 41.4
(-3) 		403.8
(3) 		280.7
(1) 		56.7
(-2.7) 		362

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 203
1 2 3 4 5 6 range
A 109.3
(-1.9)
A 		258.6
(3)
P 		117
(-1.7)
A 		166.8
(-0)
P 		165.9
(-0.1)
A 		76.9
(-3)
A 		182
B 75
(-3)
A 		232.2
(3)
P 		137.8
(-0.6)
A 		141.6
(-0.5)
A 		133.5
(-0.8)
M 		76
(-3)
A 		157
C 55.9
(-3)
A 		230.8
(3)
P 		165.3
(0.8)
A 		154.5
(0.4)
M 		215.2
(2.5)
P 		56.8
(-3)
A 		175
avr 80.1
(-2.6) 		240.5
(3) 		140
(-0.5) 		154.3
(-0) 		171.5
(0.6) 		69.9
(-3) 		171
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 19.1
(-2.3)
A 		100.7
(3)
P 		77.3
(1.5)
P 		8.7
(-3)
A 		92
B 14.4
(-3)
A 		95.3
(3)
P 		66.4
(0.9)
P 		19.6
(-2.6)
A 		81
C 8.7
(-2.7)
A 		104.1
(3)
P 		90.6
(2.2)
P 		3.1
(-3)
A 		101
avr 14.1
(-2.8) 		100
(3) 		78.1
(1.5) 		10.5
(-3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 20.1
(-2.9)
A 		92
(3)
P 		25.5
(-2.4)
A 		42.3
(-1.1)
P 		50
(-0.4)
A 		18.7
(-3)
A 		73
B 26.1
(-3)
A 		77.3
(3)
P 		33.5
(-2.1)
A 		37.5
(-1.7)
A 		54.2
(0.3)
M 		28.3
(-2.7)
A 		51
C 20.7
(-2.8)
A 		70.4
(3)
P 		42.1
(-0.3)
A 		31.1
(-1.6)
M 		69.6
(2.9)
P 		19
(-3)
A 		51
avr 22.3
(-3) 		79.9
(3) 		33.7
(-1.8) 		37
(-1.4) 		57.9
(0.7) 		22
(-3) 		58
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189549_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6
Sequence Type Exemplar sequence
Representative Public ID T06A4.1 NCBI
Target Description T06A4.1 /REP_DB=WormBase Gene ID /WP=CE18210 /TR=Q9TZH1 /GB=AAC67473.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=carboxypeptidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182009(10), NM_058379(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182009 NCBI Caenorhabditis elegans T06A4.1b (carboxypeptidase) mRNA, complete cds. 10/11 A 
NM_058379 NCBI Caenorhabditis elegans T06A4.1a (carboxypeptidase) mRNA, complete cds. 9/11 A 
SNAP00000004752 ENSEMBL cdna:SNAP chromosome:CEL140:I:770969:785068:1 10/11 None
GENEFINDER00000004753 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:770969:779037:1 9/11 None
T06A4.1b ENSEMBL cdna:known chromosome:CEL140:I:770894:776346:1 gene:T06A4.1 10/11 A 
T06A4.1a ENSEMBL cdna:known chromosome:CEL140:I:770969:776303:1 gene:T06A4.1 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:770888-775495(+) 100.0 100.0
Public Domain and Genome References
Ensembl T06A4.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_871809 T06A4.1b [Caenorhabditis elegans] gb|AAO21390.1| Hypothetical protein T06A4.1b [Caenorhabditis elegans] 0.0
blast NP_490780 T06A4.1a [Caenorhabditis elegans] gb|AAC67473.3| Hypothetical protein T06A4.1a [Caenorhabditis elegans] 0.0
blast CAE61324 Hypothetical protein CBG05161 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.7E-8
Pfam IPR000834 EMBL-EBI Peptidase M14, carboxypeptidase A 2.6E-69
Pfam IPR003146 EMBL-EBI Peptidase M14A, carboxypeptidase activation peptide 1.4E-9
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.7E-8
Pfam IPR000834 EMBL-EBI Peptidase M14, carboxypeptidase A 1.2E-67
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.7E-8
Sequence
Target Sequence 
>C. ELEGANS:189549_AT
gcattcctactcccaaatgtggttgataccatatggacacagaaaacagagttatccaca
ggactaccatactggactgcggccattggctctgcgagcaactaaggcgttgtatgagtt
gtatgggacgaaatatcaagtcgggaccggcgctgatttgatgtacgaagcttctggagg
atcacatgattgggctaaggggcagcttaaggtcccctatgcctacctgattgagctccg
accaaagaacaccatgatgggccacggcttccttctccccgaacgagaaattgtgcccac
tggactggaaaccttcgaatccatcaaagtggttgccgacgagcttgtggcgcaatttgt
ggaacctgtgattcgagccaagctgaccacaaccacaaagcccgcaatcccaccgtatcg
gcgtggaagctccaaccacagaggagcccaccaccactactacggaggaacctacaacta
ccacaacgaccgaggagcctac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCATTCCTACTCCCAAATGTGGTTG 654 307 1017 Antisense
CAGGACTACCATACTGGACTGCGGC 627 207 1075 Antisense
GGCCATTGGCTCTGCGAGCAACTAA 246 543 1097 Antisense
GGACCGGCGCTGATTTGATGTACGA 227 523 1160 Antisense
TACCTGATTGAGCTCCGACCAAAGA 279 647 1240 Antisense
AGAACACCATGATGGGCCACGGCTT 145 73 1262 Antisense
AATTGTGCCCACTGGACTGGAAACC 496 177 1305 Antisense
AAGTGGTTGCCGACGAGCTTGTGGC 93 159 1343 Antisense
AACCTGTGATTCGAGCCAAGCTGAC 472 147 1379 Antisense
CAATCCCACCGTATCGGCGTGGAAG 224 179 1421 Antisense
CTACCACAACGACCGAGGAGCCTAC 501 225 1494 Antisense
Sequence Source Affymetrix Proprietary Database