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[189544_at] cysteine protease

Description F57F5.1 /REP_DB=WormBase Gene ID /WP=CE05999 /TR=Q20950 /GB=CAB00098.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=cysteine protease
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 50      4grp_highest_8_8 > 33      4grp_high_8_8 > 50      6grp_highest_nonorm > 07      6grp_total > 32      4grp_total > 61      6grp_high1_sub > 03_04      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15514
1 2 3 4 range
A 16871.7
(3)
P 		13182.4
(1.6)
P 		11731.8
(1)
P 		1494.3
(-3)
P 		15377
B 16311.4
(3)
P 		12361.8
(1.4)
P 		11779.9
(1.2)
P 		1487
(-3)
P 		14824
C 16430.8
(3)
P 		11560.5
(1.1)
P 		11368.9
(1)
P 		1357.4
(-3)
P 		15073
avr 16538
(3) 		12368.2
(1.3) 		11626.9
(1) 		1446.2
(-3) 		15092

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12341
1 2 3 4 5 6 range
A 17992.6
(3)
P 		13068.9
(0.4)
P 		16887.7
(2.4)
P 		14065.5
(0.9)
P 		11539.7
(-0.4)
P 		6631.1
(-3)
P 		11362
B 14706.2
(0.9)
P 		13757
(0.5)
P 		18972.5
(3)
P 		16257.8
(1.7)
P 		11678
(-0.6)
P 		6688.5
(-3)
P 		12284
C 13615.6
(1)
P 		13765.1
(1.1)
P 		16755.5
(3)
P 		15946.5
(2.5)
P 		12364.6
(0.3)
P 		7100.1
(-3)
P 		9655
avr 15438.1
(1.8) 		13530.3
(0.8) 		17538.6
(3) 		15423.3
(1.8) 		11860.8
(-0.2) 		6806.6
(-3) 		10732
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5924
1 2 3 4 range
A 6143
(3)
P 		2861.5
(-0.3)
P 		3066.9
(-0.1)
P 		218.7
(-3)
P 		5924
B 5201.6
(3)
P 		3053.1
(0.4)
P 		2729.7
(-0)
P 		316.9
(-3)
P 		4885
C 5439.1
(3)
P 		3323.7
(0.6)
P 		3962.4
(1.3)
P 		228.9
(-3)
P 		5210
avr 5594.6
(3) 		3079.4
(0.2) 		3253
(0.4) 		254.8
(-3) 		5340

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3503
1 2 3 4 5 6 range
A 3316.8
(0.4)
P 		4647.9
(3)
P 		3677.8
(1.1)
P 		3567.3
(0.9)
P 		3482.2
(0.7)
P 		1614.5
(-3)
P 		3033
B 5117
(3)
P 		4579.1
(1.8)
P 		4607.1
(1.8)
P 		4306.1
(1.1)
P 		4744.6
(2.1)
P 		2494.1
(-3)
P 		2623
C 5046.7
(3)
P 		4200.1
(1.1)
P 		4264.6
(1.2)
P 		3206.9
(-1.1)
P 		4001
(0.7)
P 		2376.8
(-3)
P 		2670
avr 4493.5
(3) 		4475.7
(3) 		4183.2
(2.2) 		3693.4
(0.9) 		4075.9
(1.9) 		2161.8
(-3) 		2332
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189544_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13937
Sequence Type Exemplar sequence
Representative Public ID F57F5.1 NCBI
Target Description F57F5.1 /REP_DB=WormBase Gene ID /WP=CE05999 /TR=Q20950 /GB=CAB00098.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=cysteine protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073610(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073610 NCBI Caenorhabditis elegans F57F5.1 (F57F5.1) mRNA, complete cds. 11/11 None
SNAP00000039443 ENSEMBL cdna:SNAP chromosome:CEL140:V:12005189:12006969:1 11/11 None
GENEFINDER00000039449 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12005615:12006969:1 11/11 None
F57F5.1 ENSEMBL cdna:known chromosome:CEL140:V:12005615:12007058:1 gene:F57F5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12005614-12006969(+) 100.0 100.0
Public Domain and Genome References
Gene Title cysteine protease
Ensembl F57F5.1
Entrez Gene 179645 Entrez gene
SwissProt Q20950 EMBL-EBI
WormBase CE05999 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15967_AT cathepsin B-like cysteine protease, putative at
ATH1-121501:255590_AT cathepsin B-like cysteine protease, putative at
CANINE:1596254_AT similar to cathepsin B preproprotein cfa
CANINE:1583709_S_AT similar to cathepsin B preproprotein cfa
CANINE_2:CFA.21450.1.S1_S_AT similar to cathepsin B preproprotein cfa
CANINE_2:CFA.4159.1.A1_AT similar to cathepsin B preproprotein cfa
DROSOPHILA_2:1632152_AT dm
DROSGENOME1:152070_AT dm
CHICKEN:GGA.3854.1.S2_AT hypothetical gene supported by NM_205371 gga
CHICKEN:GGAAFFX.21123.1.S1_S_AT hypothetical gene supported by NM_205371 gga
HG-U95AV2:32372_AT cathepsin B hs
HG-U133A:200839_S_AT cathepsin B hs
HG-U133_PLUS_2:200839_S_AT cathepsin B hs
HG-FOCUS:200839_S_AT cathepsin B hs
HG-U133A_2:200839_S_AT cathepsin B hs
HG-U133A:200838_AT cathepsin B hs
HG-U133_PLUS_2:200838_AT cathepsin B hs
HG-U133A_2:200838_AT cathepsin B hs
HU35KSUBA:HUM_ALU_AT cathepsin B hs
HU35KSUBB:HUM_ALU_AT cathepsin B hs
HU35KSUBC:HUM_ALU_AT cathepsin B hs
HU35KSUBD:HUM_ALU_AT cathepsin B hs
HUGENEFL:HUM_ALU_AT cathepsin B hs
HC-G110:HUM_ALU_AT cathepsin B hs
HU35KSUBC:RC_W19286_AT cathepsin B hs
HU35KSUBD:RC_AA608751_I_AT cathepsin B hs
HUGENEFL:L22569_AT cathepsin B hs
U133_X3P:G4503138_3P_A_AT cathepsin B hs
U133_X3P:HS.297939.2.A1_3P_AT cathepsin B hs
U133_X3P:213275_3P_X_AT cathepsin B hs
HG-U133A_2:213274_S_AT cathepsin B hs
HG-U133_PLUS_2:213274_S_AT cathepsin B hs
HG-U133A:213274_S_AT cathepsin B hs
HG-U133A_2:213275_X_AT cathepsin B hs
HG-U133_PLUS_2:213275_X_AT cathepsin B hs
HG-U133A:213275_X_AT cathepsin B hs
HG-U133_PLUS_2:227961_AT cathepsin B hs
HG-U133B:227961_AT cathepsin B hs
HG-U133_PLUS_2:243157_AT Cathepsin B hs
HG-U133B:243157_AT Cathepsin B hs
HG-U95E:77875_AT Cathepsin B hs
HG-U95D:78806_AT Cathepsin B hs
U133_X3P:HS.135226.0.A1_3P_AT Cathepsin B hs
MOE430A:1448732_AT cathepsin B mm
MOUSE430_2:1448732_AT cathepsin B mm
MOUSE430A_2:1448732_AT cathepsin B mm
MOE430A:1417490_AT cathepsin B mm
MOUSE430_2:1417490_AT cathepsin B mm
MOUSE430A_2:1417490_AT cathepsin B mm
MOE430A:1417491_AT cathepsin B mm
MOUSE430A_2:1417491_AT cathepsin B mm
MOUSE430_2:1417491_AT cathepsin B mm
MOE430A:1417492_AT cathepsin B mm
MOUSE430A_2:1417492_AT cathepsin B mm
MOUSE430_2:1417492_AT cathepsin B mm
MU11KSUBA:M65270_F_AT cathepsin B mm
MU11KSUBB:MSA.507.0_G_AT cathepsin B mm
MU11KSUBB:MSA.507.0_AT cathepsin B mm
MG-U74AV2:95608_AT cathepsin B mm
MG-U74AV2:94831_AT cathepsin B mm
MG-U74AV2:92256_AT cathepsin B mm
MG-U74AV2:162271_F_AT cathepsin B mm
MU11KSUBB:X54966_RC_AT cathepsin B mm
MG-U74BV2:114161_AT Cathepsin B, mRNA (cDNA clone MGC:6211 IMAGE:3500700) mm
MOUSE430_2:1444987_AT Cathepsin B, mRNA (cDNA clone MGC:6211 IMAGE:3500700) mm
MOE430B:1444987_AT Cathepsin B, mRNA (cDNA clone MGC:6211 IMAGE:3500700) mm
MOE430B:1459497_AT Cathepsin B, mRNA (cDNA clone MGC:6211 IMAGE:3500700) mm
MOUSE430_2:1459497_AT Cathepsin B, mRNA (cDNA clone MGC:6211 IMAGE:3500700) mm
MU11KSUBB:MSA.5274.0_S_AT Cathepsin B, mRNA (cDNA clone MGC:6211 IMAGE:3500700) mm
MU19KSUBA:TC18517_F_AT Cathepsin B, mRNA (cDNA clone MGC:6211 IMAGE:3500700) mm
MU19KSUBB:TC26477_AT Cathepsin B, mRNA (cDNA clone MGC:6211 IMAGE:3500700) mm
MU19KSUBB:TC31042_S_AT Cathepsin B, mRNA (cDNA clone MGC:6211 IMAGE:3500700) mm
MU19KSUBC:TC41450_S_AT Cathepsin B, mRNA (cDNA clone MGC:6211 IMAGE:3500700) mm
RG-U34A:X82396_AT cathepsin B rn
RAE230A:1367646_AT Cathepsin B rn
RAT230_2:1367646_AT Cathepsin B rn
RAE230B:1395950_AT Cathepsin B rn
RAT230_2:1395950_AT Cathepsin B rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
8234 cysteine-type peptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB00098 Hypothetical protein F57F5.1 [Caenorhabditis elegans] ref|NP_506011.1| F57F5.1 [Caenorhabditis elegans] 0.0
blast CAE75367 Hypothetical protein CBG23351 [Caenorhabditis briggsae] 1.0E-172
blast CAE75367 Hypothetical protein CBG23351 [Caenorhabditis briggsae] 1.0E-171
blast AAB40605 cathepsin B-like cysteine proteinase [Ascaris suum] 5.0E-91
blast AAB40605 cathepsin B-like cysteine proteinase [Ascaris suum] 6.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR000668 EMBL-EBI Peptidase C1A, papain 1.5E-111
Pfam IPR000668 EMBL-EBI Peptidase C1A, papain 1.5E-111
Sequence
Target Sequence 
>C. ELEGANS:189544_AT
tttccatctctgctgacgatatcaatgcttgctgtggaatggtctgtggaaatggatgca
atggtggatatccaattgaagcctggcgtcactatgttaagaagggatacgttaccggag
gatcttatcaagataagactggatgcaagccatacccatatccaccatgtgaacatcacg
tcaatggaactcattacaagccatgcccatccaacatgtacccaactgacaaatgcgaac
gctcttgccaagctggatatgctctcacctaccaacaagatcttcacttcggacaatctg
catacgccgtctcaaagaaggctgctgaaatccaaaaggaaatcatgactcatggtccag
ttgaggttgcattcactgtctacgaagattttgagcattactccggaggagtctacgttc
ataccgctggagccagtcttggaggacatgccgtaaagatgctcggatggggagttgata
atggaacaccatattggctctgtgcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTCCATCTCTGCTGACGATATCAA 303 669 602 Antisense
TTGAAGCCTGGCGTCACTATGTTAA 151 701 677 Antisense
CATACCCATATCCACCATGTGAACA 67 213 752 Antisense
TGGAACTCATTACAAGCCATGCCCA 505 547 786 Antisense
CAAATGCGAACGCTCTTGCCAAGCT 132 193 831 Antisense
GATCTTCACTTCGGACAATCTGCAT 651 419 880 Antisense
CAATCTGCATACGCCGTCTCAAAGA 266 181 895 Antisense
AGTTGAGGTTGCATTCACTGTCTAC 597 63 960 Antisense
CTCCGGAGGAGTCTACGTTCATACC 422 231 1002 Antisense
GAGCCAGTCTTGGAGGACATGCCGT 576 383 1031 Antisense
GGAACACCATATTGGCTCTGTGCCA 26 529 1084 Antisense
Sequence Source Affymetrix Proprietary Database