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[189542_at] protein kinase

Description Y71F9AL.2 /REP_DB=WormBase Gene ID /WP=CE25557 /TR=Q9N4H2 /GB=AAF36003.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_total > 87      4grp_total > 11      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 14.3
(-1.9)
A 		8.4
(-2.4)
A 		2
(-3)
A 		67.5
(3)
P 		66
B 16.4
(-1.6)
A 		48.3
(3)
A 		6.4
(-3)
A 		17.7
(-1.4)
A 		42
C 30.3
(-1)
A 		6.3
(-3)
A 		22.4
(-1.6)
A 		77
(3)
P 		71
avr 20.3
(-1.6) 		21
(-1.5) 		10.3
(-3) 		54.1
(3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 76
1 2 3 4 5 6 range
A 2.3
(-3)
A 		21.5
(-1.3)
A 		21
(-1.4)
A 		2.6
(-3)
A 		33.4
(-0.3)
A 		72.1
(3)
P 		70
B 28.9
(-0.8)
A 		19.4
(-1.6)
A 		1.7
(-3)
A 		35.5
(-0.3)
A 		36.4
(-0.2)
A 		77.4
(3)
P 		76
C 11.4
(-2.1)
A 		38.8
(1.2)
A 		4.3
(-3)
A 		37.6
(1)
A 		43.2
(1.7)
A 		54.1
(3)
P 		50
avr 14.2
(-2.5) 		26.6
(-1.2) 		9
(-3) 		25.2
(-1.3) 		37.7
(-0.1) 		67.9
(3) 		59
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 5.2
(-0)
A 		1.8
(-2.2)
A 		0.5
(-3)
A 		9.9
(3)
P 		9
B 5.2
(-0.9)
A 		11.9
(3)
A 		1.5
(-3)
A 		3.8
(-1.7)
A 		10
C 10
(1.4)
A 		1.8
(-3)
A 		7.8
(0.2)
A 		13
(3)
P 		11
avr 6.8
(0.8) 		5.2
(-1) 		3.3
(-3) 		8.9
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 0.4
(-3)
A 		7.6
(-0.5)
A 		4.6
(-1.5)
A 		0.7
(-2.9)
A 		10.1
(0.4)
A 		17.5
(3)
P 		17
B 10
(-1)
A 		6.5
(-1.7)
A 		0.4
(-3)
A 		9.4
(-1.1)
A 		14.8
(0)
A 		28.9
(3)
P 		29
C 4.2
(-1.9)
A 		11.8
(0.8)
A 		1.1
(-3)
A 		7.6
(-0.7)
A 		14
(1.6)
A 		18.1
(3)
P 		17
avr 4.9
(-2.1) 		8.6
(-1) 		2
(-3) 		5.9
(-1.8) 		13
(0.4) 		21.5
(3) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189542_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.248
Sequence Type Exemplar sequence
Representative Public ID Y71F9AL.2 NCBI
Target Description Y71F9AL.2 /REP_DB=WormBase Gene ID /WP=CE25557 /TR=Q9N4H2 /GB=AAF36003.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058673(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058673 NCBI Caenorhabditis elegans Y71F9AL.2 (Y71F9AL.2) mRNA, complete cds. 11/11 None
GENEFINDER00000005905 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2915177:2918807:1 11/11 None
SNAP00000003756 ENSEMBL cdna:SNAP chromosome:CEL140:X:241814:249802:1 10/11 None
GENEFINDER00000003768 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:247577:249802:1 10/11 None
Y71F9AL.2 ENSEMBL cdna:known chromosome:CEL140:I:2915177:2918807:1 gene:Y71F9AL.2 11/11 None
AC8.2 ENSEMBL cdna:pseudogene chromosome:CEL140:X:248343:249802:1 gene:AC8.2 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2915176-2918807(+) 100.0 100.0
chrX:248309-249802(+) 49.67 49.67
Public Domain and Genome References
Gene Title protein kinase
Ensembl Y71F9AL.2
AC8.2
Entrez Gene 171863 Entrez gene
SwissProt Q9N4H2 EMBL-EBI
WormBase CE25557 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF36003 Hypothetical protein Y71F9AL.2 [Caenorhabditis elegans] ref|NP_491074.1| Y71F9AL.2 [Caenorhabditis elegans] 0.0
blast CAE59468 Hypothetical protein CBG02852 [Caenorhabditis briggsae] 1.0E-148
blast AAB03128 Hypothetical protein C49C8.1 [Caenorhabditis elegans] ref|NP_501483.1| C49C8.1 [Caenorhabditis elegans] 1.0E-145
blast AAF36003 Hypothetical protein Y71F9AL.2 [Caenorhabditis elegans] ref|NP_491074.1| Y71F9AL.2 [Caenorhabditis elegans] 1.0E-137
blast CAE59468 Hypothetical protein CBG02852 [Caenorhabditis briggsae] 1.0E-136
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 0.013
Pfam IPR000719 EMBL-EBI Protein kinase 4.3E-4
Sequence
Target Sequence 
>C. ELEGANS:189542_AT
tgatgtacatggtggccgagtcctttgtcgagctaccatggacgggatgcgaggagaccg
aagtggagcattacaagaatcagtcaaccattcttcgtctgttcagtgacgaaaagaacc
cgacccggttgaacgcagatatgagacgtcaattggacgagatttattcgatgctgaagg
cttcaaagtactatgacagtccaaattatgatgccgtttatcaattcttcaaagattcga
tggcaaaagccaaagtcacttgggatactccattcgattgggagattggcaacacgttat
ctgaggaaacgtcgaagggtgtcaagcgcaagatggggggaccatgggagaatcctgggc
tctttttcaagaaaggtcgctgggctggactcaagttcattccagaactgacaccaaaat
ctgctccaacatcaaagaagcaaaatttgccaaagtcatcttcgaaaggagggagagcag
ctcgtcaaatgagcaaagaagactcggcgaatggtgatgatgtagttgtgaatcaaggta
cgccggcgcagaagtcagcgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGATGTACATGGTGGCCGAGTCCTT 90 567 947 Antisense
GTCGAGCTACCATGGACGGGATGCG 5 473 973 Antisense
ATCAGTCAACCATTCTTCGTCTGTT 558 29 1025 Antisense
CCGACCCGGTTGAACGCAGATATGA 49 265 1066 Antisense
GACAGTCCAAATTATGATGCCGTTT 491 369 1141 Antisense
GTCACTTGGGATACTCCATTCGATT 522 465 1201 Antisense
GACCATGGGAGAATCCTGGGCTCTT 395 383 1286 Antisense
GCTGGGCTGGACTCAAGTTCATTCC 402 295 1325 Antisense
AACTGACACCAAAATCTGCTCCAAC 548 141 1352 Antisense
GTTGTGAATCAAGGTACGCCGGCGC 535 437 1471 Antisense
GTACGCCGGCGCAGAAGTCAGCGAA 307 459 1484 Antisense
Sequence Source Affymetrix Proprietary Database