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[189539_at] cytochrome P450

Description C25E10.2 /REP_DB=WormBase Gene ID /WP=CE06864 /TR=Q27471 /GB=AAA92310.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 96      6grp_highest_nonorm > 59      6grp_total > 86      4grp_total > 02      6grp_highest_sub > In      6grp_cgc > 68      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 880
1 2 3 4 range
A 34.5
(-2.9)
A 		27.6
(-3)
A 		41.8
(-2.9)
A 		722.5
(3)
P 		695
B 28.7
(-3)
A 		29.8
(-3)
A 		56.9
(-2.8)
A 		724.8
(3)
P 		696
C 33.5
(-3)
A 		55.8
(-2.8)
A 		37.4
(-3)
A 		908
(3)
P 		875
avr 32.2
(-3) 		37.7
(-3) 		45.4
(-2.9) 		785.1
(3) 		753

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7504
1 2 3 4 5 6 range
A 378.2
(-3)
P 		689.9
(-2.7)
P 		1486.7
(-2.1)
P 		3075.4
(-0.7)
P 		4354.4
(0.3)
P 		7545.1
(3)
P 		7167
B 382.9
(-3)
P 		692.6
(-2.7)
P 		1538.7
(-2)
P 		2897.1
(-0.9)
P 		5083.2
(1)
P 		7470.8
(3)
P 		7088
C 368.5
(-3)
P 		740.5
(-2.7)
P 		2020.2
(-1.7)
P 		3352.5
(-0.6)
P 		4055.6
(-0.1)
P 		7872.7
(3)
P 		7504
avr 376.5
(-3) 		707.7
(-2.7) 		1681.9
(-1.9) 		3108.3
(-0.7) 		4497.7
(0.4) 		7629.5
(3) 		7253
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 149
1 2 3 4 range
A 12.5
(-2.6)
A 		6
(-3)
A 		10.9
(-2.7)
A 		105.8
(3)
P 		100
B 9.1
(-2.9)
A 		7.4
(-3)
A 		13.2
(-2.8)
A 		154.5
(3)
P 		147
C 11.1
(-3)
A 		16
(-2.8)
A 		13
(-2.9)
A 		153.1
(3)
P 		142
avr 10.9
(-2.9) 		9.8
(-3) 		12.4
(-2.9) 		137.8
(3) 		128

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2716
1 2 3 4 5 6 range
A 69.7
(-3)
P 		245.3
(-2.4)
P 		323.8
(-2.1)
P 		780
(-0.6)
P 		1314
(1.2)
P 		1837
(3)
P 		1767
B 133.2
(-3)
P 		230.6
(-2.8)
P 		373.6
(-2.5)
P 		767.4
(-1.6)
P 		2065.2
(1.4)
P 		2785.8
(3)
P 		2653
C 136.6
(-3)
P 		226
(-2.8)
P 		514.2
(-2.1)
P 		674.2
(-1.7)
P 		1312.3
(-0.2)
P 		2635.5
(3)
P 		2499
avr 113.2
(-3) 		234
(-2.7) 		403.9
(-2.2) 		740.5
(-1.4) 		1563.8
(0.8) 		2419.4
(3) 		2306
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189539_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11512
Sequence Type Exemplar sequence
Representative Public ID C25E10.2 NCBI
Target Description C25E10.2 /REP_DB=WormBase Gene ID /WP=CE06864 /TR=Q27471 /GB=AAA92310.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072941(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072941 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-33B1) (cyp-33B1) mRNA, complete cds. 11/11 None
SNAP00000038735 ENSEMBL cdna:SNAP chromosome:CEL140:V:9043917:9046264:1 11/11 None
GENEFINDER00000038745 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9039080:9046234:1 11/11 None
C25E10.2 ENSEMBL cdna:known chromosome:CEL140:V:9043917:9046264:1 gene:C25E10.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9043917-9046265(+) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-33B1
Ensembl C25E10.2
Entrez Gene 182888 Entrez gene
SwissProt Q27471 EMBL-EBI
WormBase CE35248 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:20278_S_AT cytochrome P450 71A19, putative (CYP71A19) at
ATGENOME1:20277_I_AT cytochrome P450 71A20, putative (CYP71A20) at
ATH1-121501:254767_S_AT cytochrome P450 71A19, putative (CYP71A19) at
MG-U74AV2:102084_F_AT cytochrome P450, family 2, subfamily c, polypeptide 38 mm
MOE430A:1452501_AT cytochrome P450, family 2, subfamily c, polypeptide 38 mm
MOUSE430A_2:1452501_AT cytochrome P450, family 2, subfamily c, polypeptide 38 mm
MOUSE430_2:1452501_AT cytochrome P450, family 2, subfamily c, polypeptide 38 mm
RT-U34:M31031MRNA_F_AT cytochrome P450, family 2, subfamily c, polypeptide 7 rn
RG-U34A:M31031MRNA_F_AT cytochrome P450, family 2, subfamily c, polypeptide 7 rn
RT-U34:RC_AA945573_F_AT cytochrome P450, family 2, subfamily c, polypeptide 7 rn
RG-U34A:RC_AA945573_F_AT cytochrome P450, family 2, subfamily c, polypeptide 7 rn
RT-U34:M18335_F_AT cytochrome P450, family 2, subfamily c, polypeptide 7 rn
RG-U34A:M18335_F_AT cytochrome P450, family 2, subfamily c, polypeptide 7 rn
RG-U34A:M14775_S_AT cytochrome P450, family 2, subfamily c, polypeptide 7 rn
RAE230A:1370241_AT cytochrome P450, family 2, subfamily c, polypeptide 7 rn
RAT230_2:1370241_AT cytochrome P450, family 2, subfamily c, polypeptide 7 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA92310 Cytochrome p450 family protein 33B1 [Caenorhabditis elegans] ref|NP_505342.2| CYtochrome P450 family member (cyp-33B1) [Caenorhabditis elegans] 0.0
blast CAE72082 Hypothetical protein CBG19168 [Caenorhabditis briggsae] 0.0
blast AAK68203 Serpentine receptor, class sx protein 34, isoform b [Caenorhabditis elegans] ref|NP_505340.1| Serpentine Receptor, class SX family member (srsx-34) [Caenorhabditis elegans] 0.0
blast AAA92309 Serpentine receptor, class sx protein 34, isoform a [Caenorhabditis elegans] ref|NP_505341.1| Serpentine Receptor, class SX family member (srsx-34) [Caenorhabditis elegans] 0.0
blast AAA98571 Cytochrome p450 family protein 33A1 [Caenorhabditis elegans] ref|NP_504988.1| CYtochrome P450 family member (cyp-33A1) [Caenorhabditis elegans] 1.0E-123
Protein Families
Method ID Description E-Value
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-135
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-118
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 2.5E-78
Pfam IPR001128 EMBL-EBI Cytochrome P450 3.7E-60
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505342.2 1 2-20
C25E10.2 1 2-20
SNAP00000038735 1 2-20
GENEFINDER00000038745 15 33-55,70-92,105-122,132-154,166-188,208-230,280-302,312-331,343-361,395-417,455-477,487-509,530-552,572-594,601-623
Sequence
Target Sequence 
>C. ELEGANS:189539_AT
cgtgtgtttcgatatctgggcggcgggatttgacactctcacgaacactgttggcttcct
tatcgcctacgctataaattatccggaaatgcagatgttgattcatcaggaaatagacaa
ctatcttgcgcatcatagtcgtcttctcacgttagcagacaaaaatgcactcgtgtactt
caatgcttttgccaatgaagctcaacgagtttcaaatattcttccaatgaacttaccaca
tgcattaaccagagacgttaagctgaaaggatatcatttgaagaaaggaacaggtgtcat
tcatcaaattgctaacgtcatgaccgatgagactattttcaaagacagtcagcgtttcga
cccaaatcgatttattgacgaaaatggaaaactgaaaaagattgaggaactgtgcccgtt
ctccatgggaaaacgacagtgtattggtgaaggacttgcaagaatggaaatattccttct
agctgcaaatctattcaattattttgagtttcttccggcatcagacggattaccttcact
ctacaaagatttcagtctggtatcccatgtca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGTGTGTTTCGATATCTGGGCGGCG 289 245 993 Antisense
GGATTTGACACTCTCACGAACACTG 501 509 1018 Antisense
ATCTTGCGCATCATAGTCGTCTTCT 388 31 1115 Antisense
ATAGTCGTCTTCTCACGTTAGCAGA 102 3 1127 Antisense
CACTCGTGTACTTCAATGCTTTTGC 331 195 1160 Antisense
CAAAGACAGTCAGCGTTTCGACCCA 3 187 1332 Antisense
GATTGAGGAACTGTGCCCGTTCTCC 272 435 1392 Antisense
GGAAATATTCCTTCTAGCTGCAAAT 436 527 1458 Antisense
TTTTGAGTTTCTTCCGGCATCAGAC 38 677 1494 Antisense
GCATCAGACGGATTACCTTCACTCT 117 309 1510 Antisense
GATTTCAGTCTGGTATCCCATGTCA 116 429 1540 Antisense
Sequence Source Affymetrix Proprietary Database