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[189536_at]

Description PAR2.3 /REP_DB=WormBase Gene ID /WP=CE00847 /TR=SW:P45894 /GB=AAA50618.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Protein kinase
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 98      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 92      6grp_total > 98      4grp_total > 04      6grp_highest_sub > In      6grp_cgc > 78      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 381
1 2 3 4 range
A 159
(-2.5)
P 		128.2
(-3)
P 		206.4
(-1.7)
P 		487.3
(3)
P 		359
B 230.4
(-0.8)
P 		157.4
(-2.6)
P 		139
(-3)
P 		391.9
(3)
P 		253
C 231.5
(-1.3)
P 		123.4
(-3)
P 		161.6
(-2.4)
P 		504.3
(3)
P 		381
avr 207
(-1.7) 		136.3
(-3) 		169
(-2.4) 		461.2
(3) 		325

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 347
1 2 3 4 5 6 range
A 153.5
(-3)
P 		180.8
(-2.5)
P 		157.2
(-2.9)
P 		257.9
(-1)
P 		426.3
(2.2)
P 		466.2
(3)
P 		313
B 170.8
(-2.1)
P 		174.9
(-2)
P 		119.6
(-3)
P 		275.4
(-0.2)
P 		375.7
(1.7)
P 		448
(3)
P 		328
C 165.3
(-3)
P 		246.7
(-0.6)
P 		165.1
(-3)
P 		214.6
(-1.5)
P 		331.9
(1.9)
P 		368.1
(3)
P 		203
avr 163.2
(-2.7) 		200.8
(-1.9) 		147.3
(-3) 		249.3
(-0.8) 		378
(1.9) 		427.4
(3) 		280
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 57.9
(1.2)
P 		27.8
(-3)
P 		53.9
(0.6)
P 		71.3
(3)
P 		44
B 73.5
(1.8)
P 		38.9
(-2.2)
P 		32.2
(-3)
P 		83.5
(3)
P 		51
C 76.6
(2)
P 		35.5
(-3)
P 		56.3
(-0.5)
P 		85
(3)
P 		50
avr 69.3
(1.6) 		34.1
(-3) 		47.5
(-1.2) 		79.9
(3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 139
1 2 3 4 5 6 range
A 28.3
(-3)
P 		64.3
(-0.8)
P 		34.2
(-2.6)
P 		65.4
(-0.8)
P 		128.6
(3)
P 		113.5
(2.1)
P 		100
B 59.4
(-1.7)
P 		58.2
(-1.7)
P 		29
(-3)
P 		72.9
(-1.1)
P 		152.6
(2.4)
P 		167
(3)
P 		138
C 61.3
(-1.6)
P 		75.3
(-0.5)
P 		42
(-3)
P 		43.2
(-2.9)
P 		107.4
(1.8)
P 		123.2
(3)
P 		81
avr 49.7
(-2.1) 		65.9
(-1.1) 		35.1
(-3) 		60.5
(-1.5) 		129.5
(2.7) 		134.6
(3) 		100
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189536_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7689
Sequence Type Exemplar sequence
Representative Public ID PAR2.3 NCBI
Target Description PAR2.3 /REP_DB=WormBase Gene ID /WP=CE00847 /TR=SW:P45894 /GB=AAA50618.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171217(11), NM_171216(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171217 NCBI Caenorhabditis elegans AMP-Activated Kinase family member (aak-1) (aak-1) mRNA, complete cds. 11/11 None
NM_171216 NCBI Caenorhabditis elegans AMP-Activated Kinase family member (aak-1) (aak-1) mRNA, complete cds. 11/11 None
GENEFINDER00000027079 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8757138:8761425:-1 11/11 None
SNAP00000027074 ENSEMBL cdna:SNAP chromosome:CEL140:III:8757151:8760989:-1 11/11 None
PAR2.3b.2 ENSEMBL cdna:known chromosome:CEL140:III:8756909:8760992:-1 gene:PAR2.3 11/11 None
PAR2.3b.1 ENSEMBL cdna:known chromosome:CEL140:III:8756909:8760060:-1 gene:PAR2.3 11/11 None
PAR2.3a ENSEMBL cdna:known chromosome:CEL140:III:8757138:8760992:-1 gene:PAR2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8757133-8760946(-) 97.91 100.0
Public Domain and Genome References
Ensembl PAR2.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_741254 AMP-Activated Kinase family member (aak-1) [Caenorhabditis elegans] 0.0
blast NP_741255 AMP-Activated Kinase family member (aak-1) [Caenorhabditis elegans] 0.0
blast P45894 Putative serine/threonine-protein kinase PAR2.3 0.0
blast ABA54413 Amp-activated kinase protein 1, isoform a [Caenorhabditis elegans] 0.0
blast AAR06927 AMP-activated protein kinase alpha subunit 2 [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 6.0E-69
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 5.0E-69
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-69
hanks 2.2.7 CaMK Group; CaMK II KIN1/SNF1/Nim1; AKIN10 3.0E-89
hanks 2.2.7 CaMK Group; CaMK II KIN1/SNF1/Nim1; AKIN10 2.0E-89
hanks 1.1.1 AGC group; AGC I Cyclic nucleotide regulated protein kinase (PKA & PKG); cAPKa 1.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.1E-94
Pfam IPR000719 EMBL-EBI Protein kinase 2.1E-94
Pfam IPR000719 EMBL-EBI Protein kinase 4.6E-30
Pfam IPR000719 EMBL-EBI Protein kinase 2.1E-94
Sequence
Target Sequence 
>C. ELEGANS:189536_AT
gaattcgtgcatgtcttgatccagttgagaccatgaaacatgtttttttgagtttaaaat
ctgttgatatggaatggaaagtactcagtatgtatcatattattgttcgtagcaaaccga
ctccaattaatccggatcctgtgaaagtatctcttcaattatttgctctcgataaaaaag
agaataacaaaggatatttactcgatttcaaaggactcactgaagacgaagaagctgttc
caccgagtcgttgtcgttcccgtgctgcgtccgtatcagtaactctcgccaaatcgaaaa
gtgatttaaatggaaatagctcgaaagttccaatgtctccactatctccaatgtctccaa
tttctccgtctgtcaacattccaaaagttcgcgttgatgacgcggacgcctcgctgaaat
catcgttgaacagcagtatctacatggctgatattgagaactcaatggaaagtcttgatg
aagtttcaactcaatcttcggaacccgaagctccaattcgttctcagacgatggaattct
ttgcaacttgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTCGTGCATGTCTTGATCCAGT 41 325 1298 Antisense
GTAGCAAACCGACTCCAATTAATCC 132 421 1406 Antisense
TCTCTTCAATTATTTGCTCTCGATA 331 613 1447 Antisense
TGCTGCGTCCGTATCAGTAACTCTC 174 581 1560 Antisense
GTATCAGTAACTCTCGCCAAATCGA 213 455 1570 Antisense
TTTCTCCGTCTGTCAACATTCCAAA 226 671 1658 Antisense
TCGCGTTGATGACGCGGACGCCTCG 622 593 1686 Antisense
CGCCTCGCTGAAATCATCGTTGAAC 452 257 1704 Antisense
GTTTCAACTCAATCTTCGGAACCCG 45 445 1780 Antisense
AAGCTCCAATTCGTTCTCAGACGAT 107 151 1805 Antisense
ACGATGGAATTCTTTGCAACTTGCC 522 93 1825 Antisense
Sequence Source Affymetrix Proprietary Database