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[189533_at] DCMP Deaminase

Description ZK643.2 /REP_DB=WormBase Gene ID /WP=CE00441 /TR=SW:P30648 /GB=CAA77473.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=DCMP Deaminase
Cluster 6grp_total > 95      4grp_total > 51      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 218
1 2 3 4 range
A 222.3
(2.7)
A 		185.1
(-0)
A 		144.5
(-3)
A 		226.7
(3)
A 		82
B 170.8
(-2.1)
A 		315.2
(3)
A 		195.4
(-1.2)
A 		145.5
(-3)
A 		170
C 289.7
(0.7)
A 		314.8
(1.5)
A 		362.3
(3)
P 		171.8
(-3)
A 		191
avr 227.6
(0.1) 		271.7
(3) 		234.1
(0.5) 		181.3
(-3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 382
1 2 3 4 5 6 range
A 224.4
(-1.3)
A 		344.1
(1)
A 		134.2
(-3)
A 		447.9
(2.9)
A 		451
(3)
P 		153.1
(-2.6)
P 		317
B 311.8
(-0.3)
A 		206.7
(-2.1)
A 		148.5
(-3)
P 		246.5
(-1.4)
P 		344.7
(0.2)
P 		516.4
(3)
P 		368
C 210.5
(-1.8)
A 		136.5
(-3)
A 		413.1
(1.6)
A 		305.9
(-0.2)
P 		274
(-0.7)
P 		493.6
(3)
P 		357
avr 248.9
(-2.3) 		229.1
(-3) 		231.9
(-2.9) 		333.4
(0.9) 		356.6
(1.8) 		387.7
(3) 		159
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 80.9
(3)
A 		40.2
(-2.1)
A 		37.8
(-2.4)
A 		33.2
(-3)
A 		48
B 54.5
(0)
A 		77.9
(3)
A 		45.3
(-1.2)
A 		31
(-3)
A 		47
C 95.9
(1.1)
A 		90.5
(0.8)
A 		126.3
(3)
P 		29
(-3)
A 		97
avr 77.1
(3) 		69.5
(2) 		69.8
(2) 		31.1
(-3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 163
1 2 3 4 5 6 range
A 41.4
(-2.3)
A 		122.4
(2.2)
A 		29.2
(-3)
A 		113.6
(1.7)
A 		136.1
(3)
P 		37.3
(-2.5)
P 		107
B 108.5
(-0.2)
A 		68.8
(-1.7)
A 		36.1
(-3)
P 		65.3
(-1.9)
P 		140
(1)
P 		192.6
(3)
P 		157
C 78
(-1.2)
A 		41.7
(-3)
A 		105.1
(0.1)
A 		61.5
(-2)
P 		88.7
(-0.7)
P 		165.2
(3)
P 		124
avr 76
(-1.5) 		77.6
(-1.3) 		56.8
(-3) 		80.1
(-1.1) 		121.6
(2.2) 		131.7
(3) 		75
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189533_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6938
Sequence Type Exemplar sequence
Representative Public ID ZK643.2 NCBI
Target Description ZK643.2 /REP_DB=WormBase Gene ID /WP=CE00441 /TR=SW:P30648 /GB=CAA77473.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=DCMP Deaminase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066579(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066579 NCBI Caenorhabditis elegans ZK643.2 (ZK643.2) mRNA, complete cds. 11/11 None
SNAP00000011221 ENSEMBL cdna:SNAP chromosome:CEL140:III:8956478:8957479:1 11/11 None
ZK643.2 ENSEMBL cdna:known chromosome:CEL140:III:8956478:8957479:1 gene:ZK643.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8956473-8957475(+) 97.98 100.0
Public Domain and Genome References
Gene Title DCMP Deaminase
Ensembl ZK643.2
Entrez Gene 191373 Entrez gene
SwissProt P30648 EMBL-EBI
WormBase CE00441 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA77473 Hypothetical protein ZK643.2 [Caenorhabditis elegans] ref|NP_498980.1| ZK643.2 [Caenorhabditis elegans] sp|P30648|DCTD_CAEEL Probable deoxycytidylate deaminase (dCMP deaminase) 1.0E-113
blast CAE62709 Hypothetical protein CBG06863 [Caenorhabditis briggsae] 2.0E-80
blast YP_053628 deoxycytidylate deaminase [Mesoplasma florum L1] gb|AAT75744.1| deoxycytidylate deaminase [Mesoplasma florum L1] 1.0E-34
Protein Domains
Database ID Description E-Value
Pfam IPR002125 EMBL-EBI Cytidine/deoxycytidylate deaminase, zinc-binding region 1.2E-44
Sequence
Target Sequence 
>C. ELEGANS:189533_AT
gtgccacccattacatctgatgaattttgtgaaacttccggctgctttggcgattctgca
aatcttcacacaaccctggaacggctcaaaatcaatataaattctgatgccaaaaagcta
gtcgatacaaatggtgatttaaagaaacatcaacgattccttcgaatagcaaaagtgacg
tcactccggagtaaggatccaaacacacaagttgggtgtgttattgtggataaagacaat
tgcattgtgtcggttggatacaatggatttccgattggggtcgatgatgacgtttttcga
tgggataaagaagatccagaagacaataaacatctttatgttgttcatgccgagatgaat
gccataataaataaacgatgcactactcttcacgattgtacagtctacgtcactttgttc
ccttgcaataaatgcgctcaaatgttgatccagtcccgagtcaaaaaagtctacttccta
gaaaatcgtgatgaattggcatttcgagcttcaaaaaagatgctcgatcatgcgaggttg
ccatacgaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCCACCCATTACATCTGATGAAT 93 479 16 Antisense
AATTTTGTGAAACTTCCGGCTGCTT 561 173 38 Antisense
GGCTGCTTTGGCGATTCTGCAAATC 681 537 55 Antisense
ATTCTGCAAATCTTCACACAACCCT 175 11 68 Antisense
ACACAACCCTGGAACGGCTCAAAAT 468 109 83 Antisense
GACGTCACTCCGGAGTAAGGATCCA 12 379 192 Antisense
TAAACGATGCACTACTCTTCACGAT 274 641 387 Antisense
GATTGTACAGTCTACGTCACTTTGT 685 433 409 Antisense
GTCACTTTGTTCCCTTGCAATAAAT 94 465 424 Antisense
AATGTTGATCCAGTCCCGAGTCAAA 639 163 456 Antisense
GATCATGCGAGGTTGCCATACGAAC 182 419 541 Antisense
Sequence Source Affymetrix Proprietary Database