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[189527_at] aminotransferase

Description T09B4.8 /REP_DB=WormBase Gene ID /WP=CE13471 /TR=O02158 /GB=AAB53010.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=aminotransferase
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 13      6grp_highest_nonorm > 60      6grp_total > 24      4grp_total > 59      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1327
1 2 3 4 range
A 698.1
(1.2)
P 		964.5
(2.9)
P 		976.6
(3)
P 		72.6
(-3)
A 		904
B 761.6
(0.3)
P 		991.1
(1.3)
P 		1365.8
(3)
P 		38.4
(-3)
A 		1327
C 726.3
(0.5)
P 		1216.3
(2.9)
P 		1231.6
(3)
P 		39.4
(-3)
A 		1192
avr 728.7
(0.6) 		1057.3
(2.3) 		1191.3
(3) 		50.1
(-3) 		1141

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 448
1 2 3 4 5 6 range
A 449.4
(1.1)
P 		590.9
(3)
P 		513.4
(2)
P 		311.4
(-0.7)
P 		195.7
(-2.3)
P 		142.7
(-3)
P 		448
B 521.8
(2.9)
P 		530.4
(3)
P 		378.9
(0.6)
P 		318.7
(-0.4)
P 		166
(-2.8)
P 		152.9
(-3)
P 		378
C 559.5
(3)
P 		506.1
(2.2)
P 		367.7
(0.1)
P 		272.3
(-1.3)
P 		224.9
(-2)
P 		160.9
(-3)
P 		399
avr 510.2
(2.5) 		542.5
(3) 		420
(1.1) 		300.8
(-0.7) 		195.5
(-2.3) 		152.2
(-3) 		390
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 423
1 2 3 4 range
A 254.2
(3)
P 		209.4
(1.9)
P 		255.3
(3)
P 		10.6
(-3)
A 		245
B 242.9
(1.6)
P 		244.8
(1.6)
P 		316.5
(3)
P 		8.2
(-3)
A 		308
C 240.4
(0.3)
P 		349.7
(1.9)
P 		429.3
(3)
P 		6.6
(-3)
A 		423
avr 245.8
(1.4) 		268
(1.8) 		333.7
(3) 		8.5
(-3) 		325

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 175
1 2 3 4 5 6 range
A 82.8
(-1.4)
P 		210.1
(3)
P 		111.8
(-0.4)
P 		79
(-1.5)
P 		59.1
(-2.2)
P 		34.7
(-3)
P 		175
B 181.6
(3)
P 		176.5
(2.8)
P 		92
(-1.3)
P 		84.4
(-1.7)
P 		67.4
(-2.5)
P 		57
(-3)
P 		125
C 207.4
(3)
P 		154.4
(0.9)
P 		93.6
(-1.4)
P 		54.8
(-3)
P 		72.8
(-2.3)
P 		53.9
(-3)
P 		154
avr 157.3
(2) 		180.3
(3) 		99.1
(-0.7) 		72.7
(-1.9) 		66.4
(-2.2) 		48.5
(-3) 		132
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189527_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2316
Sequence Type Exemplar sequence
Representative Public ID T09B4.8 NCBI
Target Description T09B4.8 /REP_DB=WormBase Gene ID /WP=CE13471 /TR=O02158 /GB=AAB53010.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=aminotransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059376(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059376 NCBI Caenorhabditis elegans T09B4.8 (aminotransferase) mRNA, complete cds. 9/11 A 
SNAP00000036037 ENSEMBL cdna:SNAP chromosome:CEL140:I:6172972:6174666:-1 9/11 None
GENEFINDER00000036047 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6172972:6174666:-1 9/11 None
T09B4.8 ENSEMBL cdna:known chromosome:CEL140:I:6172873:6174676:-1 gene:T09B4.8 9/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6172739-6174595(-) 100.0 100.0
Public Domain and Genome References
Gene Title aminotransferase
Ensembl T09B4.8
Entrez Gene 172301 Entrez gene
SwissProt O02158 EMBL-EBI
WormBase CE33176 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:17594_S_AT alanine--glyoxylate aminotransferase, putative / beta-alanine-pyruvate aminotransferase, putative / AGT, putative at
ATGENOME1:17593_R_AT alanine--glyoxylate aminotransferase, putative / beta-alanine-pyruvate aminotransferase, putative / AGT, putative at
ATGENOME1:19858_S_AT alanine--glyoxylate aminotransferase, putative / beta-alanine-pyruvate aminotransferase, putative / AGT, putative at
ATH1-121501:252855_AT alanine--glyoxylate aminotransferase, putative / beta-alanine-pyruvate aminotransferase, putative / AGT, putative at
CANINE:1588425_AT similar to alanine-glyoxylate aminotransferase 2 precursor cfa
CANINE_2:CFA.15562.1.A1_AT similar to alanine-glyoxylate aminotransferase 2 precursor cfa
CANINE_2:CFAAFFX.28696.1.S1_S_AT similar to alanine-glyoxylate aminotransferase 2 precursor cfa
DROSOPHILA_2:1628611_AT dm
DROSGENOME1:154290_AT dm
CHICKEN:GGAAFFX.2137.1.S1_S_AT similar to alanine-glyoxylate aminotransferase 2 precursor; beta-alanine-pyruvate aminotransferase; beta-ALAAT II gga
HU35KSUBB:RC_AA010205_AT alanine-glyoxylate aminotransferase 2 hs
HU35KSUBB:RC_R10287_AT alanine-glyoxylate aminotransferase 2 hs
HU35KSUBB:RC_N65959_AT alanine-glyoxylate aminotransferase 2 hs
U133_X3P:HS.193228.0.S1_3P_AT alanine-glyoxylate aminotransferase 2 hs
HG-U133_PLUS_2:229229_AT alanine-glyoxylate aminotransferase 2 hs
HG-U133B:229229_AT alanine-glyoxylate aminotransferase 2 hs
HG-U95B:43594_AT alanine-glyoxylate aminotransferase 2 hs
HG-U95B:58981_AT alanine-glyoxylate aminotransferase 2 hs
HG-U95C:60480_AT alanine-glyoxylate aminotransferase 2 hs
HG-U95B:53401_R_AT Alanine-glyoxylate aminotransferase 2 hs
MG-U74BV2:114160_AT alanine-glyoxylate aminotransferase 2 mm
MOE430B:1435838_AT alanine-glyoxylate aminotransferase 2 mm
MOUSE430_2:1435838_AT alanine-glyoxylate aminotransferase 2 mm
MG-U74BV2:113525_AT alanine-glyoxylate aminotransferase 2 mm
RG-U34A:AB002584_AT alanine-glyoxylate aminotransferase 2 rn
RAE230A:1368659_AT alanine-glyoxylate aminotransferase 2 rn
RAT230_2:1368659_AT alanine-glyoxylate aminotransferase 2 rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8483 transaminase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB53010 Hypothetical protein T09B4.8 [Caenorhabditis elegans] ref|NP_491777.3| T09B4.8 [Caenorhabditis elegans] 0.0
blast CAE67402 Hypothetical protein CBG12888 [Caenorhabditis briggsae] 0.0
blast AAL13781 LD24726p [Drosophila melanogaster] 1.0E-139
Protein Domains
Database ID Description E-Value
Pfam IPR005814 EMBL-EBI Aminotransferase class-III 1.3E-41
Pfam IPR005814 EMBL-EBI Aminotransferase class-III 4.7E-73
Sequence
Target Sequence 
>C. ELEGANS:189527_AT
ggattcgaatcacaagatgccttgccagacatggtaactatggcaaaaggaatcggaaac
ggattcccattgggagctgttgtcactagtaaagaaattgccgactctttcaacaaatct
ctatacttcaatacatatggtggaaatccattagcttccgttgttggaaaagctgtattg
gaagtgattgaagaggagaagcttcaggaaaacagcgctgttgttggagattacttcttg
aaacaacttgcagcaattgatgatgcgacaattggagatgttagaggaaaagggttaatg
atcggagtggaacttatcgatgagcaagggaaaccacttgccgcagccaaaacagcagct
atcttcgaagacactaagaaccaaggcctactgatcggaaaaggaggtatccatggaaac
gttctccgtatcaaaccaccaatgtgtattactaaaaaggatgtcgattttgctgcgccg
tcatttcttcatttcgatattaccaaattcttgttttttgttgttgtttcaaactataat
tcaaatattcaaaattctttcgggttctatgaaattattgttccaaaacgcttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATTCGAATCACAAGATGCCTTGC 290 509 907 Antisense
GATGCCTTGCCAGACATGGTAACTA 215 415 922 Antisense
TTCCCATTGGGAGCTGTTGTCACTA 425 697 970 Antisense
TGCCGACTCTTTCAACAAATCTCTA 496 585 1005 Antisense
AAATCTCTATACTTCAATACATATG 465 117 1021 Antisense
GAAATCCATTAGCTTCCGTTGTTGG 596 361 1049 Antisense
GAAGCTTCAGGAAAACAGCGCTGTT 512 341 1104 Antisense
AGCGCTGTTGTTGGAGATTACTTCT 298 83 1120 Antisense
TACTTCTTGAAACAACTTGCAGCAA 474 645 1138 Antisense
ATTCAAAATTCTTTCGGGTTCTATG 166 9 1453 Antisense
GAAATTATTGTTCCAAAACGCTTCA 562 361 1477 Antisense
Sequence Source Affymetrix Proprietary Database