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[189526_at] dehydrogenase

Description F25G6.5 /REP_DB=WormBase Gene ID /WP=CE09641 /TR=SW:O16925 /GB=AAC25798.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster 6grp_total > 0      4grp_total > 01      6grp_total_sub > Hat      6grp_cgc > 54      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 47.2
(-2.5)
A 		38.6
(-3)
A 		60.2
(-1.7)
A 		138.8
(3)
A 		100
B 51.6
(0.2)
A 		23.7
(-2.9)
A 		22.7
(-3)
A 		77
(3)
P 		54
C 46.9
(-3)
P 		53.1
(-2.3)
P 		64.7
(-1)
A 		99.8
(3)
P 		53
avr 48.6
(-2.1) 		38.5
(-3) 		49.2
(-2) 		105.2
(3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 43.6
(-1)
A 		35
(-3)
P 		54.3
(1.5)
A 		51.9
(1)
A 		58.2
(2.5)
P 		60.5
(3)
P 		26
B 47.9
(0.3)
A 		70.9
(3)
P 		44.5
(-0.2)
P 		20.7
(-3)
A 		48.1
(0.3)
P 		57
(1.3)
P 		50
C 61.5
(-1.9)
M 		88.3
(3)
P 		70.7
(-0.2)
A 		55.7
(-3)
M 		65.5
(-1.2)
P 		61.3
(-2)
P 		33
avr 51
(-0.8) 		64.7
(3) 		56.5
(0.8) 		42.8
(-3) 		57.3
(1) 		59.6
(1.6) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 17.2
(1.4)
A 		8.4
(-3)
A 		15.7
(0.7)
A 		20.3
(3)
A 		12
B 16.5
(3)
A 		5.9
(-2.7)
A 		5.3
(-3)
A 		16.4
(2.9)
P 		11
C 15.5
(-2.8)
P 		15.3
(-3)
P 		22.5
(3)
A 		16.8
(-1.8)
P 		7
avr 16.4
(1.9) 		9.9
(-3) 		14.5
(0.5) 		17.8
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 8
(-3)
A 		12.5
(-0.2)
P 		11.8
(-0.6)
A 		13.2
(0.2)
A 		17.6
(3)
P 		14.7
(1.2)
P 		10
B 16.7
(0.7)
A 		23.6
(3)
P 		10.8
(-1.2)
P 		5.5
(-3)
A 		19.5
(1.6)
P 		21.2
(2.2)
P 		18
C 22.8
(1.4)
M 		27
(3)
P 		18
(-0.4)
A 		11.2
(-3)
M 		21.2
(0.8)
P 		20.5
(0.5)
P 		16
avr 15.8
(0.2) 		21
(3) 		13.5
(-1.1) 		10
(-3) 		19.4
(2.1) 		18.8
(1.8) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189526_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11739
Sequence Type Exemplar sequence
Representative Public ID F25G6.5 NCBI
Target Description F25G6.5 /REP_DB=WormBase Gene ID /WP=CE09641 /TR=SW:O16925 /GB=AAC25798.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072804(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072804 NCBI Caenorhabditis elegans F25G6.5 (dehydrogenase) mRNA, complete cds. 11/11 None
SNAP00000003977 ENSEMBL cdna:SNAP chromosome:CEL140:V:8552647:8553753:1 11/11 None
F25G6.5 ENSEMBL cdna:known chromosome:CEL140:V:8552647:8553753:1 gene:F25G6.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000003990 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8552647-8553754(+) 100.0 100.0
Public Domain and Genome References
Gene Title dehydrogenase
Gene Symbol tag-57
Ensembl F25G6.5
Entrez Gene 184934 Entrez gene
SwissProt O16925 EMBL-EBI
WormBase CE09641 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:265012_AT short-chain dehydrogenase/reductase (SDR) family protein at
ATGENOME1:13359_AT short-chain dehydrogenase/reductase (SDR) family protein at
CANINE:1591622_AT similar to hydroxysteroid dehydrogenase like 1 cfa
CANINE_2:CFAAFFX.30561.1.S1_AT similar to hydroxysteroid dehydrogenase like 1 cfa
DROSOPHILA_2:1629716_A_AT dm
DROSGENOME1:146386_AT dm
CHICKEN:GGA.1854.1.S1_S_AT hydroxysteroid dehydrogenase like 1 gga
HG-U133B:223248_AT hydroxysteroid dehydrogenase like 1 hs
HG-U133_PLUS_2:223248_AT hydroxysteroid dehydrogenase like 1 hs
HU35KSUBA:RC_AA459968_AT hydroxysteroid dehydrogenase like 1 hs
HU35KSUBA:H25167_AT hydroxysteroid dehydrogenase like 1 hs
HU35KSUBB:RC_F02027_AT hydroxysteroid dehydrogenase like 1 hs
U133_X3P:HS.171937.0.S2_3P_X_AT hydroxysteroid dehydrogenase like 1 hs
U133_X3P:HS.171937.0.S2_3P_AT hydroxysteroid dehydrogenase like 1 hs
U133_X3P:223248_3P_AT hydroxysteroid dehydrogenase like 1 hs
HG-U95B:54777_AT hydroxysteroid dehydrogenase like 1 hs
HG-U95B:54779_AT hydroxysteroid dehydrogenase like 1 hs
MG-U74BV2:116136_AT hydroxysteroid dehydrogenase like 1 mm
MOE430B:1454996_AT hydroxysteroid dehydrogenase like 1 mm
MOUSE430_2:1454996_AT hydroxysteroid dehydrogenase like 1 mm
MG-U74BV2:116655_AT Hydroxysteroid dehydrogenase like 1, mRNA (cDNA clone MGC:86138 IMAGE:6809862) mm
MU19KSUBC:TC37979_AT Hydroxysteroid dehydrogenase like 1, mRNA (cDNA clone MGC:86138 IMAGE:6809862) mm
RAE230A:1388577_AT Similar to steroid dehydrogenase-like (predicted) rn
RAT230_2:1388577_AT Similar to steroid dehydrogenase-like (predicted) rn
RAE230B:1397254_AT Similar to steroid dehydrogenase-like (predicted) rn
RAT230_2:1397254_AT Similar to steroid dehydrogenase-like (predicted) rn
RG-U34C:RC_AA818576_F_AT Similar to steroid dehydrogenase-like (predicted) rn
RG-U34C:RC_AI179136_AT Similar to steroid dehydrogenase-like (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC25798 Temporarily assigned gene name protein 57 [Caenorhabditis elegans] ref|NP_505205.1| F25G6.5 [Caenorhabditis elegans] sp|O16925|DHBY_CAEEL Putative steroid dehydrogenase F25G6.5 1.0E-168
blast CAE66201 Hypothetical protein CBG11441 [Caenorhabditis briggsae] 1.0E-100
blast CAB01114 Hypothetical protein C06B3.4 [Caenorhabditis elegans] ref|NP_506449.1| STeroid DeHydrogenase family member (stdh-1) [Caenorhabditis elegans] sp|Q17703|DHBV_CAEEL Putative steroid dehydrogenase C06B3.4 7.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 4.3E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505205.1 1 10-29
F25G6.5 1 10-29
SNAP00000003977 1 10-29
Sequence
Target Sequence 
>C. ELEGANS:189526_AT
gttcgttacgccatctgtgattttacaagggtatcttacgaagattacaagaggttatta
catagtctcaacgaagttgacattggaattttgataaacaatgtgggaatgtgttttgac
aatccagaagttttacacagagtggaaggtggaattgatactctcactaatgttatcaat
gttaatattttgccgttgacagctggaattcttccgcaaatgatggctagaaaatctgga
ataattgtcaatatagggtctgctgctggatcaattcatatggctaaatggtcggtttat
tcagcaaccaaaaaatacattgagtggttcacttcaattcttcaaaaagaatatgaaaat
gaaggaattatatgccagactataacaccgttacttgtttcaacaaacatgataaaaaac
ccactttcatcaattttctgcccgaatagtgattcatttgcaaagtcatcactgaataca
attggcaattcatctagcacaacagga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCGTTACGCCATCTGTGATTTTA 169 443 328 Antisense
GGTGGAATTGATACTCTCACTAATG 405 499 475 Antisense
TTTGCCGTTGACAGCTGGAATTCTT 338 669 516 Antisense
GGAATTCTTCCGCAAATGATGGCTA 388 533 532 Antisense
GTCAATATAGGGTCTGCTGCTGGAT 428 463 574 Antisense
GCTGCTGGATCAATTCATATGGCTA 602 297 589 Antisense
AATGGTCGGTTTATTCAGCAACCAA 89 155 614 Antisense
TGAGTGGTTCACTTCAATTCTTCAA 318 567 648 Antisense
TATGCCAGACTATAACACCGTTACT 16 659 698 Antisense
TCATCAATTTTCTGCCCGAATAGTG 293 621 754 Antisense
TGGCAATTCATCTAGCACAACAGGA 199 547 810 Antisense
Sequence Source Affymetrix Proprietary Database