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[189522_at] ATP citrate lyase

Description B0365.1 /REP_DB=WormBase Gene ID /WP=CE07719 /TR=P90731 /GB=CAB02690.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=ATP citrate lyase
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 65      4grp_high_8_8 > 52      6grp_highest_nonorm > 87      6grp_total > 68      4grp_total > 51      6grp_highest_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1080
1 2 3 4 range
A 867.2
(0.6)
P 		1294.2
(3)
P 		1127.8
(2.1)
P 		214.4
(-3)
A 		1080
B 808.6
(0.3)
P 		1261.5
(3)
P 		941.3
(1.1)
P 		266.3
(-3)
P 		995
C 755.1
(0.4)
P 		1155.1
(3)
P 		999.8
(2)
P 		225.2
(-3)
P 		930
avr 810.3
(0.4) 		1236.9
(3) 		1023
(1.7) 		235.3
(-3) 		1002

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 860
1 2 3 4 5 6 range
A 1048.2
(-3)
P 		1096.5
(-2.6)
P 		1241.7
(-1.3)
P 		1142.2
(-2.2)
P 		1179.5
(-1.8)
P 		1718.6
(3)
P 		670
B 1493.6
(3)
P 		874.6
(-3)
P 		1200.9
(0.2)
P 		1061.4
(-1.2)
P 		987.7
(-1.9)
P 		1391.5
(2)
P 		619
C 1313.1
(0.7)
P 		858.6
(-3)
P 		1603.2
(3)
P 		926.4
(-2.5)
P 		1357.5
(1)
P 		1341.7
(0.9)
P 		745
avr 1285
(0.8) 		943.2
(-3) 		1348.6
(1.5) 		1043.3
(-1.9) 		1174.9
(-0.4) 		1483.9
(3) 		541
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 317
1 2 3 4 range
A 315.7
(3)
P 		280.9
(2.3)
P 		294.8
(2.6)
P 		31.4
(-3)
A 		284
B 257.8
(1.7)
P 		311.6
(3)
P 		218.1
(0.8)
P 		56.7
(-3)
P 		255
C 250
(1.1)
P 		332.1
(2.7)
P 		348.5
(3)
P 		38
(-3)
P 		311
avr 274.5
(2.2) 		308.2
(3) 		287.1
(2.5) 		42
(-3) 		266

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 333
1 2 3 4 5 6 range
A 193.2
(-3)
P 		390
(2.2)
P 		270.4
(-0.9)
P 		289.7
(-0.4)
P 		355.9
(1.3)
P 		418.4
(3)
P 		225
B 519.7
(3)
P 		291.1
(-2.7)
P 		291.6
(-2.7)
P 		281.1
(-3)
P 		401.3
(0)
P 		518.9
(3)
P 		239
C 486.7
(3)
P 		262
(-1.5)
P 		408
(1.4)
P 		186.3
(-3)
P 		439.3
(2.1)
P 		449.1
(2.2)
P 		300
avr 399.9
(1.2) 		314.4
(-1.2) 		323.3
(-1) 		252.4
(-3) 		398.8
(1.2) 		462.1
(3) 		210
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189522_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14319
Sequence Type Exemplar sequence
Representative Public ID B0365.1 NCBI
Target Description B0365.1 /REP_DB=WormBase Gene ID /WP=CE07719 /TR=P90731 /GB=CAB02690.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=ATP citrate lyase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073866(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073866 NCBI Caenorhabditis elegans B0365.1 (B0365.1) mRNA, complete cds. 11/11 None
SNAP00000032427 ENSEMBL cdna:SNAP chromosome:CEL140:V:13119098:13122630:1 11/11 None
GENEFINDER00000032439 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13118043:13122630:1 11/11 None
B0365.1 ENSEMBL cdna:known chromosome:CEL140:V:13119093:13122725:1 gene:B0365.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13119097-13122630(+) 100.0 100.0
Public Domain and Genome References
Gene Title ATP citrate lyase
Ensembl B0365.1
Entrez Gene 179795 Entrez gene
SwissProt P90731 EMBL-EBI
WormBase CE07719 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:258515_AT ATP-citrate synthase, putative / ATP-citrate (pro-S-)-lyase, putative / citrate cleavage enzyme, putative at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02690 Hypothetical protein B0365.1 [Caenorhabditis elegans] ref|NP_506267.1| B0365.1 [Caenorhabditis elegans] 0.0
blast CAE64663 Hypothetical protein CBG09435 [Caenorhabditis briggsae] 0.0
blast AAB00585 Hypothetical protein D1005.1 [Caenorhabditis elegans] ref|NP_508280.1| D1005.1 [Caenorhabditis elegans] sp|P53585|ACLY_CAEEL Probable ATP-citrate synthase (ATP-citrate (pro-S-)-lyase) (Citrate cleavage enzyme) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003781 EMBL-EBI CoA-binding domain 5.4E-35
Pfam IPR005811 EMBL-EBI ATP-citrate lyase/succinyl-CoA ligase 1.4E-70
Pfam IPR003781 EMBL-EBI CoA-binding domain 5.4E-35
Pfam IPR005811 EMBL-EBI ATP-citrate lyase/succinyl-CoA ligase 1.4E-70
Sequence
Target Sequence 
>C. ELEGANS:189522_AT
tgctcactgctgatcacggaccagcagtttctggtgctcataatactatcgtctgtgctc
gcgctggaaaggacttgatatcttctctcacatctggactcctcaccatcggtgaccgat
tcggaggtgctctcgatggagctgctcgtcaattctctgaggctttcgatcaagggtggt
ctccaaatcagtttgtcggagaaatgcgcaaacgtggaactcatattatgggtatcggcc
atcgtgtcaagagcatcaataaccctgataagcgtgtcgagattctcaagagatttgctt
tgaacaaaaaggagtttgctcaagaaacaccacttctcgattatgctctggaagttgaga
agatcaccactgccaagaagccaaacctcattctaaatgttgatggagctattgccatca
tcttcgtcgacatcttgcgcaacagtgggatgttcacaacagctgaagctcaagaagtta
tcgagataggtgctctgaatggaatgtttgtgctcggtcgct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTCACTGCTGATCACGGACCAGC 621 579 2684 Antisense
CATAATACTATCGTCTGTGCTCGCG 53 215 2722 Antisense
TGTGCTCGCGCTGGAAAGGACTTGA 69 557 2737 Antisense
AAGGACTTGATATCTTCTCTCACAT 373 161 2752 Antisense
ACCGATTCGGAGGTGCTCTCGATGG 98 89 2798 Antisense
ATCGGCCATCGTGTCAAGAGCATCA 562 33 2917 Antisense
GAAACACCACTTCTCGATTATGCTC 183 357 3007 Antisense
GAAGATCACCACTGCCAAGAAGCCA 409 337 3042 Antisense
TGGAGCTATTGCCATCATCTTCGTC 249 549 3087 Antisense
GTCGACATCTTGCGCAACAGTGGGA 418 473 3109 Antisense
AATGGAATGTTTGTGCTCGGTCGCT 507 161 3181 Antisense
Sequence Source Affymetrix Proprietary Database