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[189519_at] cytochrome P450

Description R08F11.3 /REP_DB=WormBase Gene ID /WP=CE12584 /TR=O02641 /GB=AAB54245.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 07      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 40      6grp_total > 08      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 11      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 444
1 2 3 4 range
A 514.8
(3)
P 		171.6
(-2.7)
P 		158.9
(-2.9)
P 		151.2
(-3)
P 		364
B 470
(3)
P 		168.9
(-2.2)
P 		125.6
(-3)
P 		149.5
(-2.6)
P 		344
C 569.7
(3)
P 		184.9
(-2.9)
P 		187.9
(-2.9)
P 		179
(-3)
P 		391
avr 518.2
(3) 		175.1
(-2.7) 		157.5
(-3) 		159.9
(-3) 		361

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 881
1 2 3 4 5 6 range
A 1103.2
(3)
P 		606.7
(-1)
P 		595.1
(-1.1)
P 		555
(-1.5)
P 		476.6
(-2.1)
P 		366.8
(-3)
P 		736
B 1203.7
(3)
P 		689
(-0.8)
P 		594.8
(-1.4)
P 		590.7
(-1.5)
P 		447.5
(-2.5)
P 		381.8
(-3)
P 		822
C 1248.1
(3)
P 		577
(-1.9)
P 		553.2
(-2)
P 		594
(-1.7)
P 		504.9
(-2.4)
P 		418.9
(-3)
P 		829
avr 1185
(3) 		624.2
(-1.2) 		581
(-1.6) 		579.9
(-1.6) 		476.3
(-2.3) 		389.2
(-3) 		796
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 167
1 2 3 4 range
A 187.4
(3)
P 		37.2
(-2.5)
P 		41.5
(-2.3)
P 		22.1
(-3)
P 		165
B 149.9
(3)
P 		41.7
(-2.4)
P 		29.1
(-3)
P 		31.9
(-2.9)
P 		121
C 188.6
(3)
P 		53.2
(-2.1)
P 		65.5
(-1.7)
P 		30.2
(-3)
P 		158
avr 175.3
(3) 		44
(-2.4) 		45.4
(-2.3) 		28.1
(-3) 		147

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 373
1 2 3 4 5 6 range
A 203.4
(2.4)
P 		215.8
(3)
P 		129.6
(-1.1)
P 		140.8
(-0.6)
P 		143.8
(-0.4)
P 		89.3
(-3)
P 		127
B 418.8
(3)
P 		229.3
(-1.1)
P 		144.4
(-3)
P 		156.5
(-2.7)
P 		181.8
(-2.1)
P 		142.4
(-3)
P 		276
C 462.6
(3)
P 		176.1
(-2)
P 		140.8
(-2.6)
P 		119.4
(-3)
P 		163.4
(-2.2)
P 		140.2
(-2.6)
P 		343
avr 361.6
(3) 		207.1
(-0.9) 		138.3
(-2.6) 		138.9
(-2.6) 		163
(-2) 		124
(-3) 		238
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189519_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15583
Sequence Type Exemplar sequence
Representative Public ID R08F11.3 NCBI
Target Description R08F11.3 /REP_DB=WormBase Gene ID /WP=CE12584 /TR=O02641 /GB=AAB54245.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071650(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071650 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-33C8) (cyp-33C8) mRNA, complete cds. 11/11 None
SNAP00000031721 ENSEMBL cdna:SNAP chromosome:CEL140:V:3800680:3803258:-1 11/11 None
GENEFINDER00000031730 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3800680:3803496:-1 11/11 None
R08F11.3 ENSEMBL cdna:known chromosome:CEL140:V:3800606:3803268:-1 gene:R08F11.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3800679-3803258(-) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-33C8
Ensembl R08F11.3
Entrez Gene 187705 Entrez gene
SwissProt O02641 EMBL-EBI
WormBase CE12584 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:257636_AT cytochrome P450 71B22, putative (CYP71B22) at
CANINE:1582481_AT cytochrome P450 2B11 cfa
CANINE_2:CFA.3883.1.S1_AT cytochrome P450 2B11 cfa
CANINE_2:CFAAFFX.8512.1.S1_S_AT cytochrome P450 2B11 cfa
HG-U95AV2:1371_S_AT cytochrome P450, family 2, subfamily B, polypeptide 6 hs
HC-G110:1371_S_AT cytochrome P450, family 2, subfamily B, polypeptide 6 hs
HG-U133_PLUS_2:217133_X_AT cytochrome P450, family 2, subfamily B, polypeptide 6 hs
HG-U133A_2:217133_X_AT cytochrome P450, family 2, subfamily B, polypeptide 6 hs
HG-U133A:217133_X_AT cytochrome P450, family 2, subfamily B, polypeptide 6 hs
HG-U133_PLUS_2:206755_AT cytochrome P450, family 2, subfamily B, polypeptide 6 hs
HG-U133A:206755_AT cytochrome P450, family 2, subfamily B, polypeptide 6 hs
HG-U133A_2:206755_AT cytochrome P450, family 2, subfamily B, polypeptide 6 hs
HU35KSUBA:M29873_S_AT cytochrome P450, family 2, subfamily B, polypeptide 6 hs
U133_X3P:G13699814_3P_AT cytochrome P450, family 2, subfamily B, polypeptide 6 hs
U133_X3P:HS.1360.1.S1_3P_X_AT Cytochrome P450, family 2, subfamily B, polypeptide 6 hs
MOE430A:1425645_S_AT cytochrome P450, family 2, subfamily b, polypeptide 10 mm
MOUSE430_2:1425645_S_AT cytochrome P450, family 2, subfamily b, polypeptide 10 mm
MOUSE430A_2:1425645_S_AT cytochrome P450, family 2, subfamily b, polypeptide 10 mm
MG-U74AV2:102701_AT cytochrome P450, family 2, subfamily b, polypeptide 10 mm
MOUSE430A_2:1422257_S_AT cytochrome P450, family 2, subfamily b, polypeptide 10 mm
MOUSE430_2:1422257_S_AT cytochrome P450, family 2, subfamily b, polypeptide 10 mm
MOE430A:1422257_S_AT cytochrome P450, family 2, subfamily b, polypeptide 10 mm
MU11KSUBA:M21856_AT cytochrome P450, family 2, subfamily b, polypeptide 10 mm
MOE430A:1451787_AT cytochrome P450, family 2, subfamily b, polypeptide 10 mm
MOUSE430A_2:1451787_AT cytochrome P450, family 2, subfamily b, polypeptide 10 mm
MOUSE430_2:1451787_AT cytochrome P450, family 2, subfamily b, polypeptide 10 mm
RN-U34:J00728CDS_F_AT Cytochrome P450, family 2, subfamily b, polypeptide 2 rn
RT-U34:J00728CDS_F_AT Cytochrome P450, family 2, subfamily b, polypeptide 2 rn
RG-U34A:J00728CDS_F_AT Cytochrome P450, family 2, subfamily b, polypeptide 2 rn
RT-U34:K01721MRNA_S_AT Cytochrome P450, family 2, subfamily b, polypeptide 2 rn
RG-U34A:K01721MRNA_S_AT Cytochrome P450, family 2, subfamily b, polypeptide 2 rn
RT-U34:L00320CDS_F_AT Cytochrome P450, family 2, subfamily b, polypeptide 2 rn
RG-U34A:L00320CDS_F_AT Cytochrome P450, family 2, subfamily b, polypeptide 2 rn
RT-U34:M11251CDS_F_AT Cytochrome P450, family 2, subfamily b, polypeptide 2 rn
RG-U34A:M11251CDS_F_AT Cytochrome P450, family 2, subfamily b, polypeptide 2 rn
RT-U34:M13234CDS_F_AT Cytochrome P450, family 2, subfamily b, polypeptide 2 rn
RG-U34A:M13234CDS_F_AT Cytochrome P450, family 2, subfamily b, polypeptide 2 rn
RT-U34:K00996MRNA_S_AT Cytochrome P450, family 2, subfamily b, polypeptide 2 rn
RG-U34A:K00996MRNA_S_AT Cytochrome P450, family 2, subfamily b, polypeptide 2 rn
RAE230A:1371076_AT Cytochrome P450, family 2, subfamily b, polypeptide 2 rn
RAT230_2:1371076_AT Cytochrome P450, family 2, subfamily b, polypeptide 2 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB54245 Hypothetical protein R08F11.3 [Caenorhabditis elegans] ref|NP_504051.1| CYtochrome P450 family member (cyp-33C8) [Caenorhabditis elegans] 0.0
blast AAB65888 Cytochrome p450 family protein 33C4 [Caenorhabditis elegans] ref|NP_503612.1| CYtochrome P450 family member (cyp-33C4) [Caenorhabditis elegans] 0.0
blast AAG24106 Cytochrome p450 family protein 33C3 [Caenorhabditis elegans] ref|NP_503615.1| CYtochrome P450 family member (cyp-33C3) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-150
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-151
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 4.6E-106
Pfam IPR001128 EMBL-EBI Cytochrome P450 4.6E-106
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504051.1 1 2-21
R08F11.3 1 2-21
SNAP00000031721 1 2-21
Sequence
Target Sequence 
>C. ELEGANS:189519_AT
tatggacggcaggcttatctaccactattatcacaattaactggaccatctgctacatta
tgaatactccgggagttcaagaaaaaatgcaggaggaaatggataaggtggtgggaggtg
gtagattggtgacaacagctgataagaatgacctaccatatatgaatgcggtgatcaatg
aggctcagagatgtgggaatattgtgccgctgaacctgttgcattgtacaacaaaggaca
ctgttatcaatggttattcagtgaaaaaggggactggagtcgttgcgcagatcagcacag
ttatgtatgatgagaagatcttccctgacccctatactttcaacccggatagattcatcg
atgacaatgggaagctggttaaaatcgaacaactcattccattctcaatcggaaaacggc
aatgcctcggagaaggacttgctcgaatggagctgttccttttcattgccaactttttga
acaggtatcacatctcaccaggatcctccggtcttccatcgctcgacaaatcgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGGACGGCAGGCTTATCTACCAC 563 653 911 Antisense
GGCTTATCTACCACTATTATCACAA 583 537 922 Antisense
TTAACTGGACCATCTGCTACATTAT 599 683 947 Antisense
GTGGGAATATTGTGCCGCTGAACCT 60 489 1103 Antisense
TGCCGCTGAACCTGTTGCATTGTAC 163 585 1115 Antisense
GAGTCGTTGCGCAGATCAGCACAGT 285 399 1187 Antisense
TATACTTTCAACCCGGATAGATTCA 650 655 1243 Antisense
GAACAACTCATTCCATTCTCAATCG 683 351 1297 Antisense
AACGGCAATGCCTCGGAGAAGGACT 348 117 1325 Antisense
GAACAGGTATCACATCTCACCAGGA 681 351 1389 Antisense
GTCTTCCATCGCTCGACAAATCGAA 510 467 1421 Antisense
Sequence Source Affymetrix Proprietary Database