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[189518_at]

Description Y104H12D.1 /REP_DB=WormBase Gene ID /WP=CE28102 /TR=Q9N5A1 /GB=AAF60394.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=decarboxylase
Cluster 6grp_total > 08      4grp_total > 13      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 68.7
(3)
A 		9.3
(-2.5)
A 		3.7
(-3)
A 		45.5
(0.9)
A 		65
B 11
(-3)
A 		32.9
(3)
A 		13.2
(-2.4)
A 		13
(-2.5)
A 		22
C 17.8
(-2.3)
A 		11.7
(-2.8)
A 		8.4
(-3)
A 		89.2
(3)
A 		81
avr 32.5
(0.5) 		18
(-1.6) 		8.4
(-3) 		49.2
(3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 9.5
(3)
A 		7
(0.9)
A 		2.6
(-2.8)
A 		8.4
(2.1)
A 		5.3
(-0.5)
A 		2.3
(-3)
A 		7
B 41
(3)
A 		8.7
(-1.9)
A 		3
(-2.8)
A 		2.3
(-2.9)
A 		4.4
(-2.5)
A 		1.4
(-3)
A 		40
C 9.3
(-0.1)
A 		3.5
(-2.3)
A 		8.4
(-0.5)
A 		17.6
(3)
A 		1.8
(-3)
A 		1.7
(-3)
A 		16
avr 19.9
(3) 		6.4
(-1.5) 		4.7
(-2) 		9.4
(-0.5) 		3.8
(-2.3) 		1.8
(-3) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 25
(3)
A 		2
(-2.8)
A 		1
(-3)
A 		6.7
(-1.6)
A 		24
B 3.5
(-2.2)
A 		8.1
(3)
A 		3.1
(-2.7)
A 		2.8
(-3)
A 		5
C 5.9
(-1.5)
A 		3.4
(-2.8)
A 		2.9
(-3)
A 		15
(3)
A 		12
avr 11.5
(3) 		4.5
(-1.6) 		2.3
(-3) 		8.2
(0.8) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 1.7
(0.5)
A 		2.5
(3)
A 		0.6
(-3)
A 		2.1
(1.7)
A 		1.6
(0.2)
A 		0.6
(-3)
A 		2
B 14.3
(3)
A 		2.9
(-2)
A 		0.7
(-2.9)
A 		0.6
(-3)
A 		1.8
(-2.4)
A 		0.5
(-3)
A 		14
C 3.4
(2.8)
A 		1.1
(-2)
A 		2.1
(0.1)
A 		3.5
(3)
A 		0.6
(-3)
A 		0.6
(-3)
A 		3
avr 6.5
(3) 		2.2
(-1.4) 		1.1
(-2.5) 		2.1
(-1.5) 		1.3
(-2.3) 		0.6
(-3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189518_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10441
Sequence Type Exemplar sequence
Representative Public ID Y104H12D.1 NCBI
Target Description Y104H12D.1 /REP_DB=WormBase Gene ID /WP=CE28102 /TR=Q9N5A1 /GB=AAF60394.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=decarboxylase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067650
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
Y104H12D.3 ENSEMBL cdna:known chromosome:CEL140:IV:1193076:1201846:1 gene:Y104H12D.3 A 
SNAP00000032195 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1182975:1201418:1 None
GENEFINDER00000032199 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1193076:1201449:1 None
NM_067650 NCBI Caenorhabditis elegans Y104H12D.3 (Y104H12D.3) mRNA, complete cds. A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1187822-1194328(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y104H12D.3
Entrez Gene 190868 Entrez gene
SwissProt Q966E7 EMBL-EBI
WormBase CE31632 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6520 amino acid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16831 carboxy-lyase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68403 Hypothetical protein Y104H12D.3 [Caenorhabditis elegans] ref|NP_500051.2| Y104H12D.3 [Caenorhabditis elegans] 0.0
blast CAE68261 Hypothetical protein CBG13938 [Caenorhabditis briggsae] 1.0E-118
blast CAE68261 Hypothetical protein CBG13938 [Caenorhabditis briggsae] 1.0E-115
blast XP_694140 PREDICTED: similar to sphingosine phosphate lyase 1 [Danio rerio] ref|XP_693742.1| PREDICTED: similar to sphingosine phosphate lyase 1 [Danio rerio] 1.0E-102
blast CAE65555 Hypothetical protein CBG10544 [Caenorhabditis briggsae] 2.0E-96
Protein Domains
Database ID Description E-Value
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 0.48
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 2.9E-5
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 0.48
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 2.9E-5
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 0.48
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 2.9E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000032195 1 245-264
Sequence
Target Sequence 
>C. ELEGANS:189518_AT
agatgggcgtcgcatttgacagctcggatcgagatttgagctttcgaattgctctcgaat
atttcgacgatttgcggatcctttttaatgcgaaatgctccggattggagccttggcaga
ttgtctcctacgcaatttccctatgcttcctgatcgcctacatcaaacgaatgacccgat
ctaatgagccgccacttgttcagatcagaaaatcgatattctcattccttcgatcacttc
cgtgggtccgccgaaagttggaagctgatttggcgaaagctcaggctgaaattgaggatg
aagttcatcagagtgatcatgtacgtcgaaatcagcgcgaaaaatcatcgatttttatgt
ataacacgcatccgccggcaccgaatctgacgacaaaatgcagaaaatccgatttttcgt
caatttttggttactgtagcctcaaagcagcaactctggcattgaatttcacgacaaatt
gcagaaatttcaatttttctgcgattcgtcgtggaattcagtgtttgcacagttttggcg
atacagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATGGGCGTCGCATTTGACAGCTC 512 65 662 Antisense
GGAGCCTTGGCAGATTGTCTCCTAC 537 521 768 Antisense
CTTCCTGATCGCCTACATCAAACGA 558 219 807 Antisense
GAATGACCCGATCTAATGAGCCGCC 195 315 830 Antisense
TCTAATGAGCCGCCACTTGTTCAGA 317 611 841 Antisense
TCGATATTCTCATTCCTTCGATCAC 587 603 874 Antisense
GTGGGTCCGCCGAAAGTTGGAAGCT 629 453 903 Antisense
CCGGCACCGAATCTGACGACAAAAT 132 257 1036 Antisense
TGTAGCCTCAAAGCAGCAACTCTGG 338 555 1096 Antisense
TTCAATTTTTCTGCGATTCGTCGTG 679 683 1150 Antisense
GTTTGCACAGTTTTGGCGATACAGT 15 451 1184 Antisense
Sequence Source Affymetrix Proprietary Database