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[189514_at] thiolesterase

Description C08B11.7 /REP_DB=WormBase Gene ID /WP=CE01477 /TR=SW:Q09444 /GB=CAA86665.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=thiolesterase
Cluster 6grp_highest_10_10 > 47      6grp_high1_10_10 > 73      4grp_highest_8_8 > 09      4grp_high_8_8 > 61      6grp_highest_nonorm > 66      6grp_total > 74      4grp_total > 58      6grp_cgc > 76      6grp_high1_sub > 03_04      6grp_cgc_sub > 03_04      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1604
1 2 3 4 range
A 1245.2
(-0.9)
P 		2027.8
(2.8)
P 		2074.7
(3)
P 		806.9
(-3)
P 		1268
B 1401.7
(-0.5)
P 		1928
(1.5)
P 		2333.9
(3)
P 		729.8
(-3)
P 		1604
C 1415.3
(-0.3)
P 		1979
(2)
P 		2218.4
(3)
P 		762.8
(-3)
P 		1456
avr 1354.1
(-0.6) 		1978.3
(2) 		2209
(3) 		766.5
(-3) 		1443

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 633
1 2 3 4 5 6 range
A 1589.9
(1)
P 		1163.1
(-3)
P 		1796.1
(3)
P 		1580
(1)
P 		1634.6
(1.5)
P 		1280.7
(-1.9)
P 		633
B 1529.6
(-0.3)
P 		1374.6
(-3)
P 		1709.7
(2.9)
P 		1603.5
(1)
P 		1714.6
(3)
P 		1658.5
(2)
P 		340
C 1268.3
(-2.7)
P 		1383.8
(-1.3)
P 		1534.1
(0.6)
P 		1676.8
(2.3)
P 		1240.8
(-3)
P 		1734.6
(3)
P 		494
avr 1462.6
(-0.5) 		1307.2
(-3) 		1680
(3) 		1620.1
(2) 		1530
(0.6) 		1557.9
(1) 		373
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 655
1 2 3 4 range
A 453.4
(1.7)
P 		440.2
(1.6)
P 		542.4
(3)
P 		118.1
(-3)
P 		424
B 447
(1.5)
P 		476.2
(2)
P 		540.8
(3)
P 		155.5
(-3)
P 		385
C 468.5
(0.2)
P 		569
(1.1)
P 		773.2
(3)
P 		128.6
(-3)
P 		645
avr 456.3
(1) 		495.1
(1.5) 		618.8
(3) 		134.1
(-3) 		485

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 404
1 2 3 4 5 6 range
A 293.1
(-3)
P 		413.6
(0.6)
P 		391.2
(-0.1)
P 		400.7
(0.2)
P 		493.3
(3)
P 		311.8
(-2.4)
P 		200
B 532.2
(-0.5)
P 		457.5
(-2.1)
P 		415.2
(-3)
P 		424.7
(-2.8)
P 		696.6
(3)
P 		618.4
(1.3)
P 		281
C 470.1
(0.3)
P 		422.2
(-0.9)
P 		390.5
(-1.7)
P 		337.2
(-3)
P 		401.5
(-1.4)
P 		580.7
(3)
P 		244
avr 431.8
(-1.1) 		431.1
(-1.2) 		399
(-2.5) 		387.5
(-3) 		530.5
(3) 		503.6
(1.9) 		143
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189514_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3721
Sequence Type Exemplar sequence
Representative Public ID C08B11.7 NCBI
Target Description C08B11.7 /REP_DB=WormBase Gene ID /WP=CE01477 /TR=SW:Q09444 /GB=CAA86665.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=thiolesterase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063283(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063283 NCBI Caenorhabditis elegans C08B11.7 (thiolesterase) mRNA, complete cds. 11/11 None
SNAP00000039309 ENSEMBL cdna:SNAP chromosome:CEL140:II:8038301:8039352:-1 11/11 None
GENEFINDER00000039318 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8038301:8039352:-1 11/11 None
C08B11.7 ENSEMBL cdna:known chromosome:CEL140:II:8038197:8039518:-1 gene:C08B11.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8038297-8039694(-) 100.0 100.0
Public Domain and Genome References
Gene Title thiolesterase
Gene Symbol ubh-4
Ensembl C08B11.7
Entrez Gene 174289 Entrez gene
SwissProt Q09444 EMBL-EBI
WormBase CE36913 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:264639_AT ubiquitin carboxyl-terminal hydrolase family 1 protein at
ATGENOME1:20000_AT ubiquitin carboxyl-terminal hydrolase family 1 protein at
CANINE:1583355_AT similar to ubiquitin C-terminal hydrolase UCH37 cfa
CANINE_2:CFA.1152.1.A1_S_AT similar to ubiquitin C-terminal hydrolase UCH37 cfa
CANINE_2:CFAAFFX.16541.1.S1_AT similar to ubiquitin C-terminal hydrolase UCH37 cfa
DROSOPHILA_2:1635466_AT Ubiquitin C-terminal hydrolase dm
DROSGENOME1:154255_AT Ubiquitin C-terminal hydrolase dm
CHICKEN:GGAAFFX.12236.1.S1_AT ubiquitin carboxyl-terminal hydrolase L5 gga
CHICKEN:GGAAFFX.12236.1.S1_S_AT ubiquitin carboxyl-terminal hydrolase L5 gga
HG-U133_PLUS_2:219960_S_AT ubiquitin carboxyl-terminal hydrolase L5 hs
HG-U133A:219960_S_AT ubiquitin carboxyl-terminal hydrolase L5 hs
HG-FOCUS:219960_S_AT ubiquitin carboxyl-terminal hydrolase L5 hs
HG-U133A_2:219960_S_AT ubiquitin carboxyl-terminal hydrolase L5 hs
HG-U133_PLUS_2:220083_X_AT ubiquitin carboxyl-terminal hydrolase L5 hs
HG-U133A:220083_X_AT ubiquitin carboxyl-terminal hydrolase L5 hs
HG-U133A_2:220083_X_AT ubiquitin carboxyl-terminal hydrolase L5 hs
HU35KSUBB:RC_H20703_AT ubiquitin carboxyl-terminal hydrolase L5 hs
HU35KSUBC:RC_AA451712_AT ubiquitin carboxyl-terminal hydrolase L5 hs
HU35KSUBD:RC_AA280511_AT ubiquitin carboxyl-terminal hydrolase L5 hs
U133_X3P:G7706752_3P_A_AT ubiquitin carboxyl-terminal hydrolase L5 hs
U133_X3P:G7706307_3P_AT ubiquitin carboxyl-terminal hydrolase L5 hs
U133_X3P:HS2.396940.1.S1_3P_AT ubiquitin carboxyl-terminal hydrolase L5 hs
HG-U133_PLUS_2:1570145_AT ubiquitin carboxyl-terminal hydrolase L5 hs
HG-U95C:55307_AT ubiquitin carboxyl-terminal hydrolase L5 hs
HG-U95B:58417_AT ubiquitin carboxyl-terminal hydrolase L5 hs
HU35KSUBA:RC_AA019822_AT Ubiquitin carboxyl-terminal hydrolase L5 hs
HU35KSUBB:RC_AA232488_AT Ubiquitin carboxyl-terminal hydrolase L5 hs
U133_X3P:HS.25223.0.A1_3P_AT Ubiquitin carboxyl-terminal hydrolase L5 hs
HG-U133_PLUS_2:229248_AT Ubiquitin carboxyl-terminal hydrolase L5 hs
HG-U133B:229248_AT Ubiquitin carboxyl-terminal hydrolase L5 hs
HG-U95B:43928_AT Ubiquitin carboxyl-terminal hydrolase L5 hs
HG-U95E:88396_AT Ubiquitin carboxyl-terminal hydrolase L5 hs
HG-U95E:91209_AT Ubiquitin carboxyl-terminal hydrolase L5 hs
HU35KSUBD:RC_AA600053_AT Ubiquitin carboxyl-terminal hydrolase L5 hs
MOE430A:1419453_AT ubiquitin carboxyl-terminal esterase L5 mm
MOUSE430A_2:1419453_AT ubiquitin carboxyl-terminal esterase L5 mm
MOUSE430_2:1419453_AT ubiquitin carboxyl-terminal esterase L5 mm
MU11KSUBA:AA614954_G_AT ubiquitin carboxyl-terminal esterase L5 mm
MU11KSUBA:AA614954_AT ubiquitin carboxyl-terminal esterase L5 mm
MG-U74AV2:100512_AT ubiquitin carboxyl-terminal esterase L5 mm
MG-U74BV2:114312_G_AT ubiquitin carboxyl-terminal esterase L5 mm
MG-U74BV2:114311_AT ubiquitin carboxyl-terminal esterase L5 mm
MG-U74CV2:167974_AT ubiquitin carboxyl-terminal esterase L5 mm
MOE430A:1419452_AT ubiquitin carboxyl-terminal esterase L5 mm
MOUSE430A_2:1419452_AT ubiquitin carboxyl-terminal esterase L5 mm
MOUSE430_2:1419452_AT ubiquitin carboxyl-terminal esterase L5 mm
MOE430B:1455086_AT Ubiquitin carboxyl-terminal esterase L5, mRNA (cDNA clone MGC:6295 IMAGE:2650799) mm
MOUSE430_2:1455086_AT Ubiquitin carboxyl-terminal esterase L5, mRNA (cDNA clone MGC:6295 IMAGE:2650799) mm
RAE230B:1383477_AT ubiquitin carboxyl-terminal hydrolase L5 (predicted) rn
RAT230_2:1383477_AT ubiquitin carboxyl-terminal hydrolase L5 (predicted) rn
RAE230B:1391724_AT Ubiquitin carboxyl-terminal hydrolase L5 (predicted) rn
RAT230_2:1391724_AT Ubiquitin carboxyl-terminal hydrolase L5 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6511 ubiquitin-dependent protein catabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4221 ubiquitin thiolesterase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86665 Hypothetical protein C08B11.7 [Caenorhabditis elegans] ref|NP_495684.2| C08B11.7 [Caenorhabditis elegans] sp|Q09444|UBH4_CAEEL Probable ubiquitin carboxyl-terminal hydrolase ubh-4 (Ubiquitin C-terminal hydrolase family 1 member 4) (Ubiquitin thiolesterase 4) 0.0
blast CAE67771 Hypothetical protein CBG13346 [Caenorhabditis briggsae] 1.0E-138
blast AAF50257 CG3431-PA [Drosophila melanogaster] ref|NP_524003.1| CG3431-PA [Drosophila melanogaster] gb|AAD27866.1| LD24440p [Drosophila melanogaster] gb|AAF08393.1| 26S proteasome regulatory complex subunit p37A [Drosophila melanogaster] gb|AAN71049.1| AT10619p [Drosophila melanogaster] 2.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR001578 EMBL-EBI Peptidase C12, ubiquitin carboxyl-terminal hydrolase family 1 2.5E-112
Sequence
Target Sequence 
>C. ELEGANS:189514_AT
gattgacttggatccgaatactcgcggtcattgtctttcgaacagcgaggaaattcgcac
tgttcacaatagtttttctcgtcagactcttttcgaacttgatatcaaaggcggagaatc
ggaggataactatcactttgtgacatacgtgccaatcggtaataaggtctacgagctgga
tgggcttcgtgagctcccacttgaggttgctgagttccaaaaagaacaagactggattga
ggcaattaagcccgtaattcaacaaagaatgcaaaaatacagcgaaggggagatcacgtt
caatttgatggcacttgttccaaaccgcaaacagaaactgcaggagatgatggagaactt
gattcaagcaaatgaaaataatgagcttgaagagcagatagccgatctcaataaagccat
tgctgatgaggattacaaaatggaaatgtaccgcaaggaaaacaatcgccgacgtcacaa
ttatactccgttcgtaattgaattgatgaagattcttgcaaaggaaggaaaattggttgg
acttgtcgacaacgcttatcaagccgcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTGACTTGGATCCGAATACTCGC 231 429 366 Antisense
TACTCGCGGTCATTGTCTTTCGAAC 251 647 384 Antisense
GAGGAAATTCGCACTGTTCACAATA 184 405 412 Antisense
GTTCACAATAGTTTTTCTCGTCAGA 206 445 427 Antisense
CTCGTCAGACTCTTTTCGAACTTGA 161 233 443 Antisense
ATCACTTTGTGACATACGTGCCAAT 665 27 497 Antisense
TGAGCTCCCACTTGAGGTTGCTGAG 486 567 555 Antisense
TGATGGCACTTGTTCCAAACCGCAA 528 567 671 Antisense
GATAGCCGATCTCAATAAAGCCATT 322 425 762 Antisense
ATCGCCGACGTCACAATTATACTCC 4 39 830 Antisense
GTCGACAACGCTTATCAAGCCGCTA 215 475 910 Antisense
Sequence Source Affymetrix Proprietary Database